Pyridine nucleotide levels and ratios in Aspergillus niger

1980 ◽  
Vol 26 (3) ◽  
pp. 405-408 ◽  
Author(s):  
L. Führer ◽  
C. P. Kubicek ◽  
M. Röhr

A reliable method for the extraction and assay of pyridine nucleotides (NAD, NADH, NADP, and NADPH) in filamentous fungi is presented. The method is applied to a study of the physiology of citric acid accumulation by Aspergillus niger. The measurement of "catabolic reduction charge" (NADH/(NAD + NADH)) and "anabolic reduction charge" (NADPH/(NADP + NADPH)) as a means of characterizing the metabolic state is discussed.

2002 ◽  
Vol 1 (2) ◽  
pp. 108-110 ◽  
Author(s):  
Asad-ur- Rehman ◽  
Sikander Ali ◽  
Ikram-ul- Haq

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