In vitro production of hydrogen peroxide by human monocytes obtained from jaundice patients

2001 ◽  
Vol 120 (5) ◽  
pp. A472-A472
Author(s):  
M TREGLIADALLAGO ◽  
J JUKEMURA ◽  
M MACHADO ◽  
J BARBUTO
Keyword(s):  
Hydrogen Peroxide ◽  
Human Monocytes ◽  
In Vitro Production ◽  
Production Of Hydrogen ◽  
Vitro Production

In vitro production of hydrogen peroxide by human monocytes obtained from jaundice patients

2001 ◽  
Vol 120 (5) ◽  
pp. A472
Author(s):  
Marisa Treglia-Dal-lago ◽  
Jose Jukemura ◽  
Marcel C.C. Machado ◽  
Jose A.M. Barbuto
Keyword(s):  
Hydrogen Peroxide ◽  
Human Monocytes ◽  
In Vitro Production ◽  
Production Of Hydrogen ◽  
Vitro Production

In Vitro Production and Secretion of Interleukin-1 by Human Monocytes After Surgical Trauma

1993 ◽  
pp. 651-654
Author(s):  
M. L. Vuotto ◽  
E. Bresciano ◽  
D. Mancino ◽  
M. T. Ielpo ◽  
M. R. Mosti ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Surgical Trauma ◽  
Human Monocytes ◽  
In Vitro Production ◽  
Vitro Production

THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS

1964 ◽  
Vol 47 (2) ◽  
pp. 306-313 ◽  
Author(s):  
Denis Gospodarowicz
Keyword(s):  
In Vitro Production ◽  
Vitro Production

ABSTRACT Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

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