Modulating CL secretion by changing expression of vasoactive intestinal polypeptide-1 receptor (VIP, R) in guinea pig distal colon in vitro

2001 ◽  
Vol 120 (5) ◽  
pp. A197-A198
Author(s):  
Jianjing Xue ◽  
Yu-Zhong Wang ◽  
Helen J. Cooke
Keyword(s):  
Guinea Pig ◽  
Vasoactive Intestinal Polypeptide ◽  
Distal Colon ◽  
Cl Secretion ◽  
Intestinal Polypeptide

Efffect of vasoactive intestinal polypeptide on gallbladder smooth muscle in vitro.

1978 ◽  
Vol 234 (1) ◽  
pp. E44 ◽  
Author(s):  
J P Ryan ◽  
S Ryave
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Motor Activity ◽  
Vasoactive Intestinal Polypeptide ◽  
Muscle Tone ◽  
Contractile Activity ◽  
Gastrointestinal Hormones ◽  
Resting Tension ◽  
Intestinal Polypeptide

The effect of vasoactive intestinal polypeptide (VIP) on basal and octapeptide of cholecystokinin (OP-CCK) induced tension was examined with guinea pig gallbladder smooth muscle strips in vitro. VIP alone produced dose-related decreases in resting tension and antagonized spontaneous contractile activity where present. In combination with OP-CCK, VIP decreased the expected contractile respone. The degree of antagonism depended upon the concentrations of OP-CCK and VIP. VIP had no effect on acetylcholine-induced contractions. From these observations, we propose that VIP can affect gallbladder motor activity by decreaseing smooth muscle tone and by antagonizing cholecystokinin. These findings lend further support to our proposal that gallbladder motor function may depend upon the action and interaction of the gastrointestinal hormones.

The effects of vasoactive intestinal polypeptide on the motility of human and guinea pig gallbladder

1984 ◽  
Vol 62 (4) ◽  
pp. 356-359 ◽  
Author(s):  
Thomas M. Feeley ◽  
Alexander S. Clanachan ◽  
Gerald W. Scott
Keyword(s):  
Guinea Pig ◽  
Spontaneous Activity ◽  
Vasoactive Intestinal Polypeptide ◽  
Human Gallbladder ◽  
Cholecystokinin Octapeptide ◽  
Low Concentrations ◽  
Resting Tone ◽  
Intestinal Polypeptide

The effects of several preparations of vasoactive intestinal polypeptide (VIP) on the motility of strips of human and guinea pig gallbladder were investigated in vitro. VIP (10−12 to 10−6 M) had no measurable effects on the spontaneous activity, resting tone or cholecystokinin-octapeptide induced tone of human gallbladder strips. However, VIP (10−12 to 10−6 M) caused biphasic effects on the tone of guinea pig gallbladder strips. At low concentrations (10−12 to 10−10 M) contractions were observed that became smaller at higher concentrations (10−9 to 10−8 M). At still higher concentrations (10−7 to 10−6 M) relaxations were elicited. It appears that VIP is not as potent a relaxant of gallbladder muscle as first described. Human gallbladder tissue was totally unresponsive to the VIP preparations tested.

Vasodilator action of guinea pig vasoactive intestinal polypeptide (VIP): comparison with common mammalian VIP

1992 ◽  
Vol 42 (3) ◽  
pp. 163-171 ◽  
Author(s):  
Satoru Naruse ◽  
Takashi Inoue ◽  
Tohru Mochizuki ◽  
Noboru Yanaihara
Keyword(s):  
Guinea Pig ◽  
Vasoactive Intestinal Polypeptide ◽  
Vasodilator Action ◽  
Intestinal Polypeptide

Synaptic behavior of myenteric neurons in guinea pig distal colon

1988 ◽  
Vol 255 (2) ◽  
pp. G184-G190 ◽  
Author(s):  
P. R. Wade ◽  
J. D. Wood
Keyword(s):  
Guinea Pig ◽  
Input Resistance ◽  
Distal Colon ◽  
Bathing Medium ◽  
Myenteric Neurons ◽  
Fiber Tracts ◽  
Slow Rise ◽  
Domain 3 ◽  
Myenteric Ganglia

Intracellular recording methods were used in vitro to analyze the synaptic behavior of neurons in myenteric ganglia of guinea pig distal colon. Fast excitatory postsynaptic potentials (EPSPs) were observed in a variety of types of colonic neurons. Both spontaneous and stimulus-evoked EPSPs were abolished or suppressed by addition of hexamethonium, tetrodotoxin, or elevation of Mg2+ and reduction of Ca2+ in the bathing medium. Individual neurons usually received inputs from several fiber tracts and multiple EPSPs were sometimes evoked by electrical stimulation of single-fiber tracts. Stimulus-evoked fast EPSPs were always of greater amplitude, longer duration, and longer decay time than were spontaneous fast EPSPs in the same neurons. No rundown of the fast EPSPs occurred during prolonged stimulation at frequencies up to 10 Hz. Repetitive stimulation evoked slow depolarizing potentials (slow EPSPs) in 25% of the neurons. Characteristics of the slow EPSPs were 1) slow rise times, 2) duration in the seconds time domain, 3) enhanced excitability, 4) increased input resistance, and 5) reduction of hyperpolarizing after-potentials. In general, the variety of synaptic potentials and the properties of the events were the same as found in myenteric neurons of the guinea pig small bowel. Compared with synaptic behavior of small intestinal myenteric neurons, the notable differences were absence of the rundown phenomenon for fast EPSPs in the colonic neurons and a greater incidence of spontaneously occurring fast EPSPs.

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