Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta

2001 ◽  
Vol 31 (7) ◽  
pp. 653-660 ◽  
Author(s):  
Nahid Saghir ◽  
Phillip J. Conde ◽  
Peter M. Brophy ◽  
John Barrett
Keyword(s):  
Ligand Binding ◽  
Binding Protein ◽  
Hymenolepis Diminuta ◽  
Biochemical Characterisation ◽  
Hydrophobic Ligand

A hydrophobic ligand-binding protein of theTaenia solium metacestode mediates uptake of the host lipid: Implication for the maintenance of parasitic cellular homeostasis

PROTEOMICS ◽  
2007 ◽  
Vol 7 (21) ◽  
pp. 4016-4030 ◽  
Author(s):  
Eung-Goo Lee ◽  
Seon-Hee Kim ◽  
Young-An Bae ◽  
Joon-Yong Chung ◽  
Myungkoo Suh ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Protein ◽  
Cellular Homeostasis ◽  
Hydrophobic Ligand

Echinococcus granulosus antigen B: A Hydrophobic Ligand Binding Protein at the host–parasite interface

2015 ◽  
Vol 93 ◽  
pp. 17-23 ◽  
Author(s):  
Valeria Silva-Álvarez ◽  
Ana Maite Folle ◽  
Ana Lía Ramos ◽  
Fernando Zamarreño ◽  
Marcelo D. Costabel ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Echinococcus Granulosus ◽  
Binding Protein ◽  
Antigen B ◽  
Host Parasite ◽  
Hydrophobic Ligand

A new diagnostic tool for neurocysticercosis is a member of a cestode specific hydrophobic ligand binding protein family 1

FEBS Letters ◽  
2000 ◽  
Vol 487 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Nahid Saghir ◽  
Phillip J. Conde ◽  
Peter M. Brophy ◽  
John Barrett
Keyword(s):  
Ligand Binding ◽  
Diagnostic Tool ◽  
Binding Protein ◽  
Protein Family ◽  
Hydrophobic Ligand

Characterization of hydrophobic-ligand-binding proteins of Taenia solium that are expressed specifically in the adult stage

Parasitology ◽  
2012 ◽  
Vol 139 (10) ◽  
pp. 1361-1374 ◽  
Author(s):  
M. RAHMAN ◽  
E.-G. LEE ◽  
S.-H. KIM ◽  
Y.-A. BAE ◽  
H. WANG ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Proteins ◽  
Unsaturated Fatty Acids ◽  
Genomic Structure ◽  
Taenia Solium ◽  
Binding Activity ◽  
Hymenolepis Diminuta ◽  
Lipid Uptake ◽  
Sequence Identity ◽  
Hydrophobic Ligand

SUMMARYTaenia solium, a causative agent of taeniasis and cysticercosis, has evolved a repertoire of lipid uptake mechanisms. Proteome analysis of T. solium excretory-secretory products (TsESP) identified 10 kDa proteins displaying significant sequence identity with cestode hydrophobic-ligand-binding-proteins (HLBPs). Two distinct 362- and 352-bp-long cDNAs encoding 264- and 258-bp-long open reading frames (87 and 85 amino acid polypeptides) were isolated by mining the T. solium expressed sequence tags and a cDNA library screening (TsHLBP1 and TsHLBP2; 94% sequence identity). They clustered into the same clade with those found in Moniezia expansa and Hymenolepis diminuta. Genomic structure analysis revealed that these genes might have originated from a common ancestor. Both the crude TsESP and bacterially expressed recombinant proteins exhibited binding activity toward 1-anilinonaphthalene-8-sulfonic acid (1,8-ANS), which was competitively inhibited by oleic acid. The proteins also bound to cis-parinaric acid (cPnA) and 16-(9-anthroyloxy) palmitic acid (16-AP), but showed no binding activity against 11-[(5-dimethylaminonaphthalene-1-sulfonyl) amino] undecanoic acid (DAUDA) and dansyl-DL-α-aminocaprylic acid (DACA). Unsaturated fatty acids (FAs) showed greater affinity than saturated FAs. The proteins were specifically expressed in adult worms throughout the strobila. The TsHLBPs might be involved in uptake and/or sequestration of hydrophobic molecules provided by their hosts, thus contributing to host-parasite interface interrelationships.

scholarly journals Odorant-binding protein. Characterization of ligand binding.

1990 ◽  
Vol 265 (11) ◽  
pp. 6118-6125
Author(s):  
J Pevsner ◽  
V Hou ◽  
A M Snowman ◽  
S H Snyder
Keyword(s):  
Ligand Binding ◽  
Binding Protein ◽  
Protein Characterization ◽  
Odorant Binding Protein ◽  
Odorant Binding
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