Multiplex Polymerase Chain Reaction for Detection of Herpes Simplex Virus Type 1, Type 2, Cytomegalovirus, and Varicella-Zoster Virus in Ocular Viral Infections

2003 ◽  
Vol 47 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Y Zhang
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex ◽  
Multiplex Polymerase Chain Reaction ◽  
Viral Infections ◽  
Chain Reaction ◽  
Varicella Zoster ◽  
Polymerase Chain ◽  
Simplex Virus

Multiplex polymerase chain reaction for the detection of herpes simplex virus, varicella-zoster virus and cytomegalovirus in ocular specimens

2003 ◽  
Vol 27 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Gurunadh Reddy Chichili ◽  
Sreedharan Athmanathan ◽  
Syed Farhatullah ◽  
Nibaran Gangopadhyay ◽  
Subhadra Jalali ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Varicella Zoster Virus ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Varicella Zoster ◽  
Polymerase Chain ◽  
Simplex Virus

Detection of Herpes Simplex Virus Type-1 DNA by In Situ Polymerase Chain Reaction

1995 ◽  
Vol 7 (3) ◽  
pp. 211
Author(s):  
Jee-Ho Choi ◽  
Seong-Beom Kim ◽  
Jung-Wha Suh ◽  
In-Sung Song ◽  
Jai-Kyoung Koh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus

Multiplex polymerase chain reaction for adenovirus and herpes simplex virus in eye swabs

1996 ◽  
Vol 56 (1) ◽  
pp. 41-48 ◽  
Author(s):  
R. Jackson ◽  
D.J. Morris ◽  
R.J. Cooper ◽  
A.S. Bailey ◽  
P.E. Klapper ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus

Development of a multiplex polymerase chain reaction for detection and typing of major human herpesviruses in cerebrospinal fluid

2007 ◽  
Vol 53 (10) ◽  
pp. 1117-1122 ◽  
Author(s):  
Massimiliano Bergallo ◽  
Cristina Costa ◽  
Samuela Margio ◽  
Francesca Sidoti ◽  
Maria Elena Terlizzi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Viral Infections ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus ◽  
Human Herpesviruses

Infections of the central nervous system (CNS) represent a difficult diagnostic problem for both clinicians and microbiologists. In particular, the Herpesviridae family plays a central etiological role in CNS viral infections. These diseases have acquired growing importance in the past few years owing to the increasing number of immunocompromised patients and the availability of new antiviral drugs. Prompt detection and diagnosis of CNS viral infections are critical because most infections are treatable, while a delayed recognition may lead to life-threatening conditions or severe sequelae. The traditional methods for detection of herpesviruses in CNS infections exhibit several drawbacks, whereas the polymerase chain reaction (PCR) on cerebrospinal fluid has revolutionized the neurovirology and is becoming an essential part of the diagnostic work-up of patients with suspected CNS viral infections. A sensitive multiplex PCR method was developed for the simultaneous detection of 6 human herpesviruses (human cytomegalovirus, herpes simplex virus 1, herpes simplex virus 2, Epstein–Barr virus, varicella-zoster virus, and human herpesvirus 6) with the aim of simplifying detection and reducing time and costs. The accuracy, reproducibility, specificity, and sensitivity of these assays were established.

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