Ebselen, a seleno-organic compound, protects ethanol-induced murine gastric mucosal injury in both in vivo and in vitro systems

Our recent study has shown that asymmetric dimethylarginine (ADMA) plays an important role in facilitating gastric mucosal injury by multiple factors. To explore whether the protection of rutaecarpine against gastric mucosal injury is related to reduction of ADMA content, a model of ethanol-induced gastric mucosal injury in rats was selected for this study. The ulcer index, the content of ADMA and NO, and the activity of dimethylarginine dimethylaminohydrolase (DDAH) in gastric tissues were measured in vivo after pretreatment with rutaecarpine. The in vitro effect of rutaecarpine on the release of calcitonin gene-related peptide (CGRP) and NO from isolated gastric tissues was also determined. The results showed that ethanol significantly increased the ulcer index, decreased the DDAH activity and the NO level, and elevated the ADMA level, which was attenuated by pretreatment with rutaecarpine (0.6 mg/kg or 1.2 mg/kg). In the isolated gastric tissues, rutaecarpine significantly increased the release of both CGRP and NO; the release of NO, but not CGRP, was abolished in the presence of l-NAME (10−4 mol/L). The present results suggest that rutaecarpine protects the gastric mucosa against injury induced by ethanol and that the gastroprotection of rutaecarpine is related to reduction of ADMA levels through stimulating the release of CGRP.

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Protective effect of rosmarinic acid-rich trichodesma khasianum clarke leaves against ethanol-induced gastric mucosal injury in vitro and in vivo

Phytomedicine ◽

10.1016/j.phymed.2020.153382 ◽

2021 ◽

Vol 80 ◽

pp. 153382 ◽

Cited By ~ 1

Author(s):

Guan-Yu Wang ◽

Sheng-Yi Chen ◽

Ying-Yin Chen ◽

Cheng-Jie Hong ◽

Yi-Hao Hsu ◽

...

Keyword(s):

Protective Effect ◽

Rosmarinic Acid ◽

Mucosal Injury ◽

Gastric Mucosal Injury

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Human Oligodendrocytes and Myelin In Vitro to Evaluate Developmental Neurotoxicity

International Journal of Molecular Sciences ◽

10.3390/ijms22157929 ◽

2021 ◽

Vol 22 (15) ◽

pp. 7929

Author(s):

Megan Chesnut ◽

Thomas Hartung ◽

Helena Hogberg ◽

David Pamies

Keyword(s):

Large Scale ◽

In Vitro Models ◽

Environmental Chemicals ◽

Developmental Neurotoxicity ◽

In Vivo Studies ◽

Regulatory Guidelines ◽

In Vitro Systems ◽

Human Oligodendrocytes

Neurodevelopment is uniquely sensitive to toxic insults and there are concerns that environmental chemicals are contributing to widespread subclinical developmental neurotoxicity (DNT). Increased DNT evaluation is needed due to the lack of such information for most chemicals in common use, but in vivo studies recommended in regulatory guidelines are not practical for the large-scale screening of potential DNT chemicals. It is widely acknowledged that developmental neurotoxicity is a consequence of disruptions to basic processes in neurodevelopment and that testing strategies using human cell-based in vitro systems that mimic these processes could aid in prioritizing chemicals with DNT potential. Myelination is a fundamental process in neurodevelopment that should be included in a DNT testing strategy, but there are very few in vitro models of myelination. Thus, there is a need to establish an in vitro myelination assay for DNT. Here, we summarize the routes of myelin toxicity and the known models to study this particular endpoint.

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Protein-templated gold nanoclusters as specific bio-imaging probes for the detection of Hg(ii) ions in in vivo and in vitro systems: discriminating between MDA-MB-231 and MCF10A cells

The Analyst ◽

10.1039/d0an02108c ◽

2021 ◽

Author(s):

Subhajit Chakraborty ◽

Atanu Nandy ◽

Subhadip Ghosh ◽

Nirmal Kumar Das ◽

Sameena Parveen ◽

...

Keyword(s):

Breast Cancer ◽

Human Serum Albumin ◽

Gold Nanoclusters ◽

Selective Detection ◽

Imaging Probes ◽

Bio Imaging ◽

In Vitro Systems ◽

Mcf10a Cells

Sub-nanomolar selective detection of Hg(ii) ions by protein (Human Serum Albumin, HSA) templated gold nanoclusters (AuNCs), both in in vitro as well as in vivo environments and specific endocytose behaviour towards breast cancer (BC) cell lines.

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Identification of Metabolites of the Acaricide, Tebufenpyrad, Formed in in vivo and in vitro Systems of Rats

Journal of Pesticide Science ◽

10.1584/jpestics.19.3_169 ◽

1994 ◽

Vol 19 (3) ◽

pp. 169-179 ◽

Cited By ~ 3

Author(s):

Kunihiko OGAWA ◽

Yoshio IHASHI

Keyword(s):

In Vitro Systems

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In Vitro Systems as Simulations of In Vivo Conditions: The Study of Cognition and Synaptic Plasticity in Neurotoxicologya

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.2000.tb06874.x ◽

2006 ◽

Vol 919 (1) ◽

pp. 119-132 ◽

Cited By ~ 8

Author(s):

M. E. GILBERT

Keyword(s):

Synaptic Plasticity ◽

In Vitro Systems

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The Role of Microbiology in Models of Dental Caries: Reaction Paper

Advances in Dental Research ◽

10.1177/08959374950090031001 ◽

1995 ◽

Vol 9 (3) ◽

pp. 255-269 ◽

Cited By ~ 13

Author(s):

G.H. Bowden

Keyword(s):

Process Selection ◽

Advantages And Disadvantages ◽

Test Models ◽

Plaque Development ◽

In Vitro Systems ◽

Selection Of

Models of the caries process have made significant contributions toward defining the roles of bacteria in caries. Microbiologists use a variety of in vitro systems to model aspects of the caries process. Also, in situ models in humans provide information on the microbiology of caries in vivo. These models do not involve the entire process leading to natural caries; consequently, the results from such studies are used to deduce the roles of bacteria in natural caries. Therefore, they can be described as Inferential Caries Models. In contrast, animal models and some clinical trials in humans involve natural caries and can be described as Complete Caries Models. Furthermore, these models are used in two distinct ways. They can be used as Exploratory Models to explore different aspects of the caries process, or as Test Models to determine the effects of anticaries agents. This dichotomy in approach to the use of caries models results in modification of the models to suit a particular role. For example, if we consider Exploratory Models, the in situ appliance in humans is superior to others for analyzing the microbiology of plaque development and demineralization in vivo. The chemostat and biofilm models are excellent for exploring factors influencing bacterial interactions. Both models can also be used as Test Models. The in situ model has been used to test the effects of fluoride on the microflora and demineralization, while the chemostat and biofilm models allow for the testing of antibacterial agents. Each model has its advantages and disadvantages and role in analysis of the caries process. Selection of the model depends on the scientific question posed and the limitations imposed by the conditions available for the study.

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