Termination of procollagen chain synthesis by puromycin. Evidence that assembly and secretion require a COOH-terminal extension.

A well but cyanotic newborn was found to have a mutant γ-globin chain, leading to a functionally abnormal fetal hemoglobin. A single amino acid substitution was found in a site consistent with known adult M hemoglobins. This patient showed no clinical evidence of cyanosis at 5 weeks of age as γ-chain synthesis was replaced by β-chain synthesis. A sibling born 20 months later was also cyanotic and the same mutant hemoglobin was found.

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Modulation of xylosyltransferase I expression provides a mechanism regulating glycosaminoglycan chain synthesis during cartilage destruction and repair

The FASEB Journal ◽

10.1096/fj.08-118166 ◽

2008 ◽

Vol 23 (3) ◽

pp. 813-822 ◽

Cited By ~ 21

Author(s):

Narayanan Venkatesan ◽

Lydia Barré ◽

Jacques Magdalou ◽

Didier Mainard ◽

Patrick Netter ◽

...

Keyword(s):

Cartilage Destruction ◽

Chain Synthesis

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Towards a Molecular Anchor Chain. Synthesis and Catenations of Spiro Crown Ethers

Synthesis ◽

10.1055/s-1997-4467 ◽

1997 ◽

Vol 1997 (04) ◽

pp. 480-488 ◽

Cited By ~ 9

Author(s):

Peter R. Ashton ◽

Thomas Horn ◽

Stephan Menzer ◽

Jon A. Preece ◽

Neil Spencer ◽

...

Keyword(s):

Crown Ethers ◽

Chain Synthesis

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Partial characterization of the shift from IgG to IgA synthesis in the clonal differentiation of human leukemic bone marrow-derived lymphocytes.

Journal of Experimental Medicine ◽

10.1084/jem.142.3.549 ◽

1975 ◽

Vol 142 (3) ◽

pp. 549-559 ◽

Cited By ~ 21

Author(s):

R A Rudders ◽

R Ross

Keyword(s):

B Cell ◽

B Lymphocyte ◽

Plasma Cells ◽

Lymphocytic Leukemia ◽

B Cell Differentiation ◽

Cell Staining ◽

Clonal Origin ◽

Switch Mechanism ◽

Sequential Labeling ◽

Chain Synthesis

An unusual B-cell proliferation was noted in an individual (Tun) which was characterized by the presence of two separate populations of chronic lymphocytic leukemia (CLL) cell staining on the surface and in the cytoplasm for either IgG(k) or IgA(k). Utilizing an idiotypic antiserum prepared from the associated serum monoclonal IgG(k) protein the idiotype was detected on the surface and in the cytoplasm of both the IgG- and IgA-bearing cell populations. These observations are consistent with a common clonal origin and a switch mechanism involving IgG and IgA synthesis. Sequential-labeling of Surface Ig and intracellular Ig with antisera conjugated to opposite fluorochromes documented the progressive maturation of the terminal differentiation of the IgA-bearing cell population at a level before morphologically distinct plasma cells. The distribution and pattern of surface and cytoplasmic IgG and IgA staining in individual cells suggest that the direction of switching is from IgG to IgA synthesis. The demonstration of shared idiotypic specificity between the IgG- and IgA-bearing populations is consistent with a transition in Ig heavy chain synthesis resulting from an alternation in the CH gene. It is concluded that certain CLL clones may manifest a switch from IgG to IgA synthesis at a level of B-cell differentiation which encompasses both the B lymphocyte and the Ig-synthesizing plasma cell.

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