Chromogranin A, the major catecholamine storage vesicle soluble protein. Multiple size forms, subcellular storage, and regional distribution in chromaffin and nervous tissue elucidated by radioimmunoassay.

A water-soluble protein fraction of nervous tissue was prepared by extraction of rabbit spinal cord with sodium citrate at pH 4.3. Characterization by nitrogen content and paper electrophoresis showed it to be a mixture of basic proteins. This extract demonstrated encephalitogenic activity when injected into rabbits. The most suitable technique for the measurement of serum antibody to the rabbit cord antigen proved to be the precipitation of antigen-antibody complexes by 40 per cent saturated ammonium sulfate. Antibody could not be demonstrated by the techniques of complement fixation, quantitative precipitation, and Ouchterlony plates. The early appearance of circulating antibody occurred almost exclusively in rabbits that subsequently developed EAE. Specificity of the antibodies for nervous tissue was demonstrated by appropriate blocking and adsorption experiments.

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Chromogranin A: localization and stoichiometry in large dense core catecholamine storage vesicles from sympathetic nerve

Brain Research ◽

10.1016/0006-8993(91)90795-w ◽

1991 ◽

Vol 567 (2) ◽

pp. 188-196 ◽

Cited By ~ 30

Author(s):

Daniel T. O'Connor ◽

Richard L. Klein ◽

Asa K. Thureson-Klein ◽

Juan A. Barbosa

Keyword(s):

Sympathetic Nerve ◽

Chromogranin A ◽

Dense Core ◽

Storage Vesicles ◽

Catecholamine Storage

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Drug-induced release of catecholamines, soluble protein and chromogranin a from the isolated bovine adrenal gland

Biochemical Pharmacology ◽

10.1016/0006-2952(69)90014-8 ◽

1969 ◽

Vol 18 (1) ◽

pp. 101-107 ◽

Cited By ~ 13

Author(s):

F.H. Schneider

Keyword(s):

Adrenal Gland ◽

Soluble Protein ◽

Chromogranin A ◽

Drug Induced

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Ultradian variations of chromogranin A in humans

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1991.261.4.r939 ◽

1991 ◽

Vol 261 (4) ◽

pp. R939-R944 ◽

Cited By ~ 3

Author(s):

M. A. Takiyyuddin ◽

H. P. Neumann ◽

J. H. Cervenka ◽

B. Kennedy ◽

T. Q. Dinh ◽

...

Keyword(s):

Blood Pressure ◽

Soluble Protein ◽

Chromogranin A ◽

Peak Plasma ◽

Plasma Catecholamines ◽

Normal Subjects ◽

Catecholamine Secretion ◽

Secretory Vesicles ◽

Sympathoadrenal System ◽

Diurnal Variability

Chromogranin A (CgA) is an acidic soluble protein exocytotically released from virtually all neuroendocrine secretory vesicles. Here we examined spontaneous variations in CgA and catecholamine concentrations in humans. In normal subjects, basal CgA showed no day-to-day, week-to-week, or diurnal variability. Plasma CgA had significant ultradian variation in normotensives and hypertensives, and in bilaterally adrenalectomized subjects. Gender, but not age or blood pressure, influenced CgA variations, males having fewer (P less than 0.05) peaks per 8 h. Plasma catecholamines had significant ultradian variations in both controls and bilaterally adrenalectomized subjects. Within individuals, neither basal nor peak plasma CgA correlated with catecholamines, nor was there concordance between plasma CgA and catecholamine peaks. Somatostatin, a widespread inhibitor of nonsympathoadrenal neuroendocrine secretion, diminished both the frequency and amplitude of plasma CgA peaks. Thus spontaneous variations in basal CgA are not directly linked to alterations in sympathoadrenal catecholamine secretion. Furthermore, neuroendocrine secretion at sites other than the sympathoadrenal system contributes to spontaneous variations in CgA concentration.

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