scholarly journals The kinetics of transferrin endocytosis and iron uptake from transferrin in rabbit reticulocytes.

1983 ◽  
Vol 258 (15) ◽  
pp. 9108-9115 ◽  
Author(s):  
B J Iacopetta ◽  
E H Morgan
2008 ◽  
Vol 4 (3) ◽  
pp. 279-287 ◽  
Author(s):  
A.E. Ghaly ◽  
A. Snow ◽  
M. Kamal

Blood ◽  
1982 ◽  
Vol 60 (3) ◽  
pp. 635-641 ◽  
Author(s):  
JE Peppriell ◽  
JA Edwards ◽  
RM Bannerman

Abstract No significant differences of Km or Vmax could be demonstrated for the initial rate (0–3 min) of iron uptake by preparations of isolated duodenal mucosal epithelial cells from normal or sex-linked anemic (sla) mice. Isolated cells from sla mice do, however, take up significantly more iron than normal cells after 20-min incubation. Upon reincubation with various types of mobilizing agents, the amount of iron released from, and the amount of iron remaining in, cells from sla mice was significantly elevated compared to control values. The percentage of iron released was the same for both types of cells, for all mobilization media employed. The current data, in conjunction with previous studies that showed deficient mucosal to serosal transport of iron in everted duodenal sacs from sla mice, suggest that the lesion in these mice may be located between the mucosal cell and the vascular compartment rather than in the mucosal cell.


1978 ◽  
Vol 175 (1) ◽  
pp. 91-104 ◽  
Author(s):  
S M Russell ◽  
P M Harrison

Horse ferritins from different organs show heterogeneity on electrofocusing in Ampholine gradients. Both ferritin and apoferritin from liver and spleen could be fractionated with respect to surface charge by serial precipitation with (NH4)2SO4. In the ferritin fractions, increasing iron content parallels increasing isoelectric point. After removal of their iron, those fractions which originally contained most iron accumulated added iron at the fastest rates. When unfractionated ferritins from different organs were compared the average isoelectric point increased in order spleen less than liver less than kidney less than heart. The order of initial rates of iron uptake by the apoferritins was spleen greater than kidney greater than heart and initial average iron contents also followed this order. The relatively low rates of iron accumulation by iron-poor molecules may have been due to structural alteration, to degradation, to activation of the iron-rich molecules or to other factors.


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