scholarly journals Isolation and sequence of the cDNAs encoding the subunits of the isozyme form of wheat protein synthesis initiation factor 4F

1992 ◽  
Vol 267 (32) ◽  
pp. 23232-23236 ◽  
Author(s):  
M.L. Allen ◽  
A.M. Metz ◽  
R.T. Timmer ◽  
R.E. Rhoads ◽  
K.S. Browning
Keyword(s):  
Protein Synthesis ◽  
Initiation Factor ◽  
Wheat Protein ◽  
Protein Synthesis Initiation

scholarly journals Protein synthesis initiation factor eIF-4D

1989 ◽  
Vol 264 (31) ◽  
pp. 18527-18530 ◽  
Author(s):  
Z Smit-McBride ◽  
J Schnier ◽  
R J Kaufman ◽  
J W Hershey
Keyword(s):  
Protein Synthesis ◽  
Initiation Factor ◽  
Protein Synthesis Initiation

mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiation factor eIF-4E from Saccharomyces cerevisiae

1987 ◽  
Vol 7 (3) ◽  
pp. 998-1003
Author(s):  
M Altmann ◽  
C Handschin ◽  
H Trachsel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Genomic Library ◽  
Binding Protein ◽  
Initiation Factor ◽  
Cdna Clones ◽  
Yeast Saccharomyces Cerevisiae ◽  
Peptide Pattern ◽  
Protein Synthesis Initiation ◽  
Encoding Protein

We have isolated genomic and cDNA clones encoding protein synthesis initiation factor eIF-4E (mRNA cap-binding protein) of the yeast Saccharomyces cerevisiae. Their identity was established by expression of a cDNA in Escherichia coli. This cDNA encodes a protein indistinguishable from purified eIF-4E in terms of molecular weight, binding to and elution from m7GDP-agarose affinity columns, and proteolytic peptide pattern. The eIF-4E gene was isolated by hybridization of cDNA to clones of a yeast genomic library. The gene lacks introns, is present in one copy per haploid genome, and encodes a protein of 213 amino acid residues. Gene disruption experiments showed that the gene is essential for growth.

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