scholarly journals Crystallization and preliminary x-ray investigation of sarcoplasmic calcium-binding protein from Nereis diversicolor.

1987 ◽  
Vol 262 (24) ◽  
pp. 11884-11885
Author(s):  
Y S Babu ◽  
J A Cox ◽  
W J Cook
Keyword(s):  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Nereis Diversicolor ◽  
X Ray

Crystallization and preliminary X-ray diffraction analysis of human calcium-binding protein S100A12

2000 ◽  
Vol 56 (2) ◽  
pp. 189-191 ◽  
Author(s):  
Olga V. Moroz ◽  
Alfred A. Antson ◽  
G. Guy Dodson ◽  
Keith S. Wilson ◽  
Inge Skibshøj ◽  
...  
Keyword(s):  
Diffraction Analysis ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
X Ray Diffraction ◽  
X Ray

Crystallization and preliminary X-ray studies of a recombinant calcium-binding protein from Entamoeba histolytica

1998 ◽  
Vol 54 (6) ◽  
pp. 1442-1445 ◽  
Author(s):  
B. Gopal ◽  
R. Suma ◽  
M. R. N. Murthy ◽  
A. Bhattacharya ◽  
S. Bhattacharya
Keyword(s):  
Entamoeba Histolytica ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Size Exclusion ◽  
Imaging Plate ◽  
X Ray ◽  
Exclusion Chromatography ◽  
Hanging Drop Method ◽  
Unit Cell Dimensions

A calcium-binding protein (CaBP) of Entamoeba histolytica was purified from an E. coli recombinant clone carrying the CaBP gene in a pET-3c expression vector using anion-exchange and size-exclusion chromatography. Examination of the amino-acid sequence of the recombinant protein suggested that it has four independent EF-hand motifs. The protein dissolved in cacodylate buffer was crystallized using the hanging-drop method with 2-methylpentane-2,4-diol (MPD) as the precipitant. X-ray diffraction data have been collected on these crystals using a MAR Research imaging-plate detector system attached to a Rigaku RU200 rotating-anode X-ray generator. The crystals belong to the hexagonal space group P6122 with unit-cell dimensions of a = b = 96.21, c = 65.48 Å. Preliminary molecular-replacement computations suggest that the structure of this protein is likely to be similar to that of calmodulin (CAM).

Crystallization and preliminary X-ray characterization of a novel calcium-binding protein AtCBL2 fromArabidopsis thaliana

2003 ◽  
Vol 59 (6) ◽  
pp. 1079-1080 ◽  
Author(s):  
Masamichi Nagae ◽  
Akira Nozawa ◽  
Nozomu Koizumi ◽  
Hiroshi Sano ◽  
Hiroshi Hashimoto ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
X Ray
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