THE POSITION OF CARBON DIOXIDE CARBON IN SUCCINIC ACID SYNTHESIZED BY HETEROTROPHIC BACTERIA

D-Glucose was dissimilated aerobically by a strain of osmophilic yeast producing glycerol, D-arabitol, ethanol, carbon dioxide, and a small amount of succinic acid. Glucose-1-C14 gave glycerol labeled in the terminal carbons, D-arabitol labeled in carbon-1 and carbon-5, methyl labeled ethanol, and succinic acid with 30% of the labeling in the carboxyl carbons and 70% in the methylene carbons. Glucose-2-C14 gave glycerol labeled in carbon-2, D-arabitol labeled in carbon-1, carbon-2, and carbon-4, carbinol labeled ethanol, and succinic acid having 70% of the labeling in the carboxyl carbons and 30% in the methylene carbons. Labeled carbon dioxide was produced from both carbon-1 and carbon-2 labeled glucose but the specific activity of carbon dioxide from glucose-1-C14 was higher than that from glucose-2-C14. The distribution of radioactive carbon in the products is explained by assuming that glucose is dissimilated via a combination of the Embden–Meyerhof and the phosphogluconate oxidation pathways, with transketolase-catalyzed reactions playing an important part in D-arabitol formation.

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MICROBIOLOGICAL STUDIES OF APPALACHIAN PODSOL SOILS: IV. THE DECOMPOSITION OF GLUCOSE IN SOILS PREVIOUSLY TREATED WITH AMENDMENTS

Canadian Journal of Research ◽

10.1139/cjr39c-014 ◽

1939 ◽

Vol 17c (5) ◽

pp. 147-153 ◽

Cited By ~ 2

Author(s):

P. H. H. Gray ◽

C. B. Taylor

Keyword(s):

Carbon Dioxide ◽

Sodium Carbonate ◽

Heterotrophic Bacteria ◽

Rate Of Evolution ◽

Previously Treated ◽

Control Samples

A study has been made of the decomposition of glucose in two cultivated podsol soils that had previously been treated with alkaline amendments. Limestone increased, and sodium carbonate decreased, the amount of carbon dioxide produced from the control samples; limestone increased the rate of evolution both in control samples and in samples receiving glucose. The total amount of carbon dioxide produced was increased by limestone in combination with sodium carbonate but not by limestone alone. The numbers of heterotrophic bacteria developing with glucose were stimulated by limestone.

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Engineering Saccharomyces cerevisiae for Succinic Acid Production From Glycerol and Carbon Dioxide

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.00566 ◽

2020 ◽

Vol 8 ◽

Author(s):

Joeline Xiberras ◽

Mathias Klein ◽

Erik de Hulster ◽

Robert Mans ◽

Elke Nevoigt

Keyword(s):

Carbon Dioxide ◽

Saccharomyces Cerevisiae ◽

Succinic Acid ◽

Acid Production ◽

Succinic Acid Production

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Reactive extraction of succinic acid using supercritical carbon dioxide

Separation Science and Technology ◽

10.1080/01496395.2017.1405033 ◽

2017 ◽

Vol 53 (4) ◽

pp. 655-661 ◽

Cited By ~ 2

Author(s):

Marek Henczka ◽

Małgorzata Djas

Keyword(s):

Carbon Dioxide ◽

Supercritical Carbon Dioxide ◽

Succinic Acid ◽

Reactive Extraction ◽

Supercritical Carbon

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POLYHYDRIC ALCOHOL PRODUCTION BY OSMOPHILIC YEASTS: STUDIES WITH C14-LABELED GLUCOSE

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o56-052 ◽

1956 ◽

Vol 34 (3) ◽

pp. 495-501 ◽

Cited By ~ 20

Author(s):

J. F. T. Spencer ◽

A. C. Neish ◽

A. C. Blackwood ◽

H. R. Sallans

Keyword(s):

Carbon Dioxide ◽

Succinic Acid ◽

Specific Activity ◽

Polyhydric Alcohol ◽

Alcohol Production ◽

Radioactive Carbon ◽

Catalyzed Reactions

D-Glucose was dissimilated aerobically by a strain of osmophilic yeast producing glycerol, D-arabitol, ethanol, carbon dioxide, and a small amount of succinic acid. Glucose-1-C14 gave glycerol labeled in the terminal carbons, D-arabitol labeled in carbon-1 and carbon-5, methyl labeled ethanol, and succinic acid with 30% of the labeling in the carboxyl carbons and 70% in the methylene carbons. Glucose-2-C14 gave glycerol labeled in carbon-2, D-arabitol labeled in carbon-1, carbon-2, and carbon-4, carbinol labeled ethanol, and succinic acid having 70% of the labeling in the carboxyl carbons and 30% in the methylene carbons. Labeled carbon dioxide was produced from both carbon-1 and carbon-2 labeled glucose but the specific activity of carbon dioxide from glucose-1-C14 was higher than that from glucose-2-C14. The distribution of radioactive carbon in the products is explained by assuming that glucose is dissimilated via a combination of the Embden–Meyerhof and the phosphogluconate oxidation pathways, with transketolase-catalyzed reactions playing an important part in D-arabitol formation.

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