scholarly journals Assembly of the sarcoplasmic reticulum. Biosynthesis of the high affinity calcium binding protein in rat skeletal muscle cell cultures.

1980 ◽  
Vol 255 (4) ◽  
pp. 1327-1334 ◽  
Author(s):  
M. Michalak ◽  
D.H. MacLennan
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Skeletal Muscle Cell ◽  
Rat Skeletal Muscle ◽  
High Affinity

scholarly journals Assembly of the sarcoplasmic reticulum proteins in differentiating rat skeletal muscle cell cultures: localization by immunofluorescence of sarcoplasmic reticulum proteins in differentiating rat skeletal muscle cell cultures

1977 ◽  
Vol 74 (1) ◽  
pp. 287-298 ◽  
Author(s):  
AO Jorgensen ◽  
VI Kalnins ◽  
E Zubrzyca ◽  
DH MacLennan
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Primary Cultures ◽  
Longitudinal Axis ◽  
Skeletal Muscle Cell ◽  
Immunofluorescent Staining ◽  
Rat Skeletal Muscle ◽  
Multinucleated Cells

Immunofluorescent staining techniques were used to study the distribution of the Ca(2) + Mg(2+)-dependent ATPase and calsequestrin in primary cultures of differentiating rat skeletal muscle cells, grown for different periods of time under various culture conditions. In mononucleated myoblasts calsequestrin was detected after 45 h in culture whereas the ATPase was not detected until 60 h. After cell fusion began, both proteins could be identified in all multinucleated cells. Myoblasts grown for longer than 60 h in low Ca(2+) medium contained calsequestrin and the ATPase, even though they were unable to fuse. These studies at the cellular level confirm biochemical findings on the biosynthesis of calsequestrin and the ATPase. Immunofluorescent staining of myoblasts showed that calsequestrin first appears in a well-defined region of the cell near one end of the nucleus. At later times, the staining occupied progressively larger regions adjacent to the nucleus and took on a fibrous appearance. This suggests that calsequestrin first accumulates in the Golgi region and then gradually spreads throughout the cell. In contrast, the ATPase appeared to be concentrated in many small patches or foci throughout the cytoplasm and was never confined to one particular region, although some parts of the cell often stained more intensely than others. In multinucleated cells, alternating dark and fluorescent strands parallel to the longitudinal axis of the cells were evident.

Specific effect of corticoids on acetylcholine receptor expression in rat skeletal muscle cell cultures

1992 ◽  
Vol 31 (2) ◽  
pp. 285-293 ◽  
Author(s):  
J.-T. Vilquin ◽  
S. Braun ◽  
P. Labouret ◽  
G. Zuber ◽  
C. Tranchant ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Specific Effect ◽  
Skeletal Muscle Cell ◽  
Receptor Expression ◽  
Rat Skeletal Muscle
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