Preparative separation of proteins and enzymes in the mean molecular-weight range of 10,000–100,000 LiChrosorb diol® packing by high-performance size-exclusion chromatography

1979 ◽  
Vol 185 ◽  
pp. 445-452 ◽  
Author(s):  
P. Roumeliotis ◽  
K.K. Unger
Keyword(s):  
Molecular Weight ◽  
Size Exclusion Chromatography ◽  
High Performance ◽  
Size Exclusion ◽  
Preparative Separation ◽  
Molecular Weight Range ◽  
Exclusion Chromatography ◽  
The Mean ◽  
Separation Of Proteins ◽  
Mean Molecular Weight

High Performance Size Exclusion Chromatograph with Computerized Data Reduction for Analysis of Polyethylene and Oligomers

1981 ◽  
Vol 64 (4) ◽  
pp. 999-1007
Author(s):  
Roger C Snyder ◽  
Charles V Breder
Keyword(s):  
Molecular Weight ◽  
High Performance ◽  
Average Molecular Weight ◽  
Porous Silica ◽  
Size Exclusion ◽  
Dead Volume ◽  
Shape Distortion ◽  
Molecular Weight Range ◽  
Liquid Chromatograph ◽  
Exclusion Chromatography

Abstract A liquid chromatograph is described for the size exclusion chromatography (SEC) of polyethylene and oligomers. The rigid column packing material was 10 µm diameter porous silica. An infrared spectrophotometer was used as the detector. The chromatograph was designed and operated to minimize the degree of chromatographic peak shape distortion resulting from factors such as system dead volume, detector cell design, sample injection volume, and sample concentration. To compensate for peak spreading and skewing, the chromatographic data were analyzed with an IBM 370/168 computer using an APL program. The molecular weight range covered by this SEC system was 100-500 000. Precision and accuracy of Jhe calculated weight average molecular weight (Mw) values for polyethylene standards over this range were ±3 and s±4%, respectively.

Examination of Penetrable Volumes ("Pores") in A High-Performance Size-Exclusion Chromatography Matrix

1985 ◽  
Vol 43 ◽  
pp. 386-387
Author(s):  
W. E. Rigsby ◽  
W. L. Lingle ◽  
D. J. O Kane ◽  
J. Lee
Keyword(s):  
Surface Area ◽  
Size Exclusion Chromatography ◽  
High Performance ◽  
Matrix Material ◽  
Total Surface Area ◽  
Size Exclusion ◽  
Prediction Errors ◽  
The Matrix ◽  
Exclusion Chromatography ◽  
Separation Of Proteins

The separation of proteins by high performance size exclusion chromatography (HPSEC) is based upon the interaction of a protein, having an effective hydrodynamic radius, A, with "pores" in the HPSEC matrix material. Two different theoretical treatments (1, 2) yield widely different estimates of the mean "pore" radius, Ao, and have different prediction errors for A. A given "pore" can be characterized by the radius of the largest molecule that it can accomodate, Ai If A > Ai, the protein is excluded from the "pore".The "pore" diameter value quoted for TSK G3000 SW is 24.0nm (3). This is at variance with A0 calculated by either treatment using the same HPSEC data: 28.4 ± 6.3nm (1) and 0.89 ± 5.4nm (2). From a knowledge of the "pore" volume it was calculated that approximately 50% of the total surface area of the HPSEC matrix corresponded to "pore". If Ae = 28.4nm, then ≥ 25% of the total surface area of the matrix should be occupied by "pores" with radii ≥ 28.4nm. These results prompted an investigation of the structure and size of "pores" in the HPSEC matrix material TSK G3000 SW.

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