Analysis of some hydroxy fatty compounds as their trimethylsilyl ethers by gas-liquid chromatography

1971 ◽  
Vol 55 (2) ◽  
pp. 402-404 ◽  
Author(s):  
R. Kannan ◽  
A. Rajiah ◽  
M.R. Subbaram ◽  
K.T. Achaya
Keyword(s):  
Liquid Chromatography ◽  
Gas Liquid Chromatography ◽  
Trimethylsilyl Ethers

Simultaneous Determination of Urinary Estriol, Pregnanediol, and Pregnanolone by Gas-Liquid Chromatography

1969 ◽  
Vol 15 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Robert D Baynton ◽  
Donald J Campbell
Keyword(s):  
Liquid Chromatography ◽  
Glycol Succinate ◽  
Urine Specimen ◽  
Gas Liquid Chromatography ◽  
Large Hospital ◽  
Flame Ionization ◽  
Trimethylsilyl Ethers ◽  
Hydrogen Flame ◽  
Progesterone Metabolites ◽  
Temperature Programmed

Abstract A simplified method for following the urinary excretion of estriol, pregnanediol, and pregnanolone during the course of pregnancy is presented. The procedure has been used successfully in a large hospital laboratory. An important advantage is that the estrogen and progesterone metabolites are measured simultaneously, and separate extraction and gas-liquid chromatography is not required. The urine specimen is hydrolyzed enzymatically, extracted, and the steroids are converted to trimethylsilyl ethers. Temperature-programmed gas-liquid chromatography is carried out on a 2% neopentyl glycol succinate column using a hydrogen flame ionization detector.

A MODIFIED METHOD FOR THE ANALYSIS OF URINARY 17-KETOSTEROIDS, PREGNANEDIOL AND PREGNANETRIOL BY GAS LIQUID CHROMATOGRAPHY IN NORMAL SUBJECTS AND SUBJECTS WITH VARIOUS ENDOCRINE DISORDERS

1967 ◽  
Vol 54 (1) ◽  
pp. 37-50 ◽  
Author(s):  
Roberto Rivera ◽  
Ralph I. Dorfman ◽  
Enrico Forchielli
Keyword(s):  
Liquid Chromatography ◽  
Normal Subjects ◽  
Endocrine Disorders ◽  
Gas Liquid Chromatography ◽  
Modified Method ◽  
Trimethylsilyl Ethers ◽  
Normal Males ◽  
Steroid Excretion ◽  
Urinary Steroid

ABSTRACT A modified method using gas-liquid chromatography (GLC) is described for the determination of androsterone*, aetiocholanolone, dehydroepiandrosterone, pregnanediol and pregnanetriol in the same urine aliquot. After sequential enzymatic hydrolysis and solvolysis, the urine extract is separated into 3 main fractions by silica gel column chromatography; one containing mainly the 17-ketosteroids, androsterone, aetiocholanolone and dehydroepiandrosterone; a second containing pregnanediol and the third pregnanetriol. After formation of the trimethylsilyl ethers (TMSi), the respective fractions are subjected to gas-liquid chromatography for quantitation. Tritiated androsterone, pregnanediol and pregnanetriol are added prior to extraction of the urines to check for recoveries. The method is specific and reproductible and is sufficiently sensitive to permit determination of urinary steroid excretion levels as low as 0.025 mg/24 hours. Results obtained with this method in the study of normal males and females and various pathological conditions are presented.

THE GAS-LIQUID CHROMATOGRAPHY OF STEROID FORMATES; AN APPLICATION IN CONGENITAL ADRENAL HYPERPLASIA

1970 ◽  
Vol 47 (1) ◽  
pp. 55-64 ◽  
Author(s):  
H. L. J. MAKIN
Keyword(s):  
Liquid Chromatography ◽  
Periodate Oxidation ◽  
Oxygenation Index ◽  
Rapid Screening ◽  
Gas Liquid Chromatography ◽  
Adrenal Hyperplasia ◽  
Trimethylsilyl Ethers ◽  
Rapid Screening Test ◽  
Speed Up ◽  
Urinary Steroid

SUMMARY The gas-liquid chromatography (GLC) of some C19 steroid formates has been investigated in an attempt to speed up the final stages of the estimation of the 11-oxygenation index. SE-30 was found to give the best separation of the urinary C19 steroids formed after borohydride reduction and periodate oxidation of urine. Formates, acetates, propionates, n-butyrates, valerates and trimethylsilyl ethers were examined; formate esters gave the best separation of androsterone and aetiocholanolone. Estimation of the 11-oxygenation index by GLC increases the specificity and speed of single estimations and it is suggested that this method may find use as a rapid screening test for abnormal urinary steroid patterns.

Composition of the capsular polysaccharides of Clostridium perfringens as a basis for their classification by chemotypes

1975 ◽  
Vol 21 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Clarence M. Paine ◽  
Robert Cherniak
Keyword(s):  
Liquid Chromatography ◽  
Clostridium Perfringens ◽  
Analytical Procedure ◽  
Gas Liquid Chromatography ◽  
Capsular Polysaccharides ◽  
Qualitative And Quantitative ◽  
Trimethylsilyl Ethers

An analytical procedure, using gas–liquid chromatography, was developed for the identification of the per(trimethylsilyl) ethers of the constituent monosaccharides obtained from the capsular polysaccharides of Clostridium perfringens Hobbs 5, Hobbs 9, Hobbs 10, and NCTC 10578. Qualitative and quantitative differences between the major polysaccharide components enabled the differentiation of the four strains of C. perfringens investigated.

Urinary excretion of 17-ketosteroids by normally fed and severely underfed boars

1970 ◽  
Vol 48 (12) ◽  
pp. 817-823
Author(s):  
M. P. Stefanovic ◽  
H. S. Bayley ◽  
S. J. Slinger
Keyword(s):  
Growth Rate ◽  
Liquid Chromatography ◽  
Urinary Excretion ◽  
Gas Liquid Chromatography ◽  
Trimethylsilyl Ethers

Two boars were fed normally to 19 weeks of age and then underfed so that their growth was severely restricted. Two other boars were underfed to 19 weeks of age and thus did not grow; they were then normally fed and the experiment was continued until they reached the same weight as the first two boars. The excretion of 17-ketosteroids (17-KS) in the urine of the four boars was measured on 2 days each week by separating their trimethylsilyl ethers by gas–liquid chromatography. The excretion of 17-KS reached a maximum of 9.0 mg per day for the boars which were fed normally in the first part of the experiment. Reducing their allocation of feed did not have an immediate effect on the excretion of total 17-KS, but after 4 weeks the excretion declined to about 1.0 mg per day. The 17-KS excretion of the boars which were underfed initially remained less than 0.5 mg per day, but increased when the feed allocation and hence growth rate of the boars increased. However, the excretion of 17-KS by these boars did not reach the levels recorded for the boars which had grown normally from the start of the experiment.

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