Systematic procedure for the determination of the nature of the solutes prior to the selection of the mobile phase parameters for optimization of reversed-phase ion-pair chromatographic separations

Abstract A simplified, simultaneous determination of vitamins B1, B2, B3, and B6 in supplemented infant formulas was developed from a single deproteinized sample extract, with analysis by reversed-phase, ion-pair chromatography with an acidified methanol–water mobile phase. The dioctylsulfosuccinate counter-ion facilitates unique retention of the pyridine-based vitamins (niacinamide and pyridoxine) and allows for concurrent measurement of both the pyridoxal and riboflavin 5′-phosphate endogenous components of milk. Other naturally occurring undetected vitamin congeners have minimal analytical significance. UV detection is used for niacinamide, and programmed fluorescence detection is used for riboflavin and the B6 vitamins. Thiamine is routinely determined sequentially under modified elution conditions.

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Reversed-phase ion-pair chromatography in determination of sinomenine contents in Sinomenium acutum

Academic Journal of Second Military Medical University ◽

10.3724/sp.j.1008.2010.00193 ◽

2010 ◽

Vol 30 (2) ◽

pp. 193-195

Author(s):

Hui WANG ◽

Qing YE ◽

Liang ZHAO ◽

Wen-jun TIAN ◽

Guo-qing ZHANG

Keyword(s):

Reversed Phase ◽

Ion Pair ◽

Ion Pair Chromatography ◽

Sinomenium Acutum

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Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19940569 ◽

1994 ◽

Vol 59 (3) ◽

pp. 569-574 ◽

Cited By ~ 1

Author(s):

Josef Královský ◽

Marta Kalhousová ◽

Petr Šlosar

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Uv Spectra ◽

Optimum Conditions ◽

Linear Calibration ◽

Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

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Determination of ambenonium chloride in serum by reversed-phase ion-pair liquid chromatography

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/0378-4347(87)80426-7 ◽

1987 ◽

Vol 421 ◽

pp. 407-411 ◽

Cited By ~ 7

Author(s):

C. Tharasse-Block ◽

C. Chabenat ◽

P. Boucly ◽

J. Marchand

Keyword(s):

Liquid Chromatography ◽

Reversed Phase ◽

Ion Pair

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Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

E-Journal of Chemistry ◽

10.1155/2011/360431 ◽

2011 ◽

Vol 8 (1) ◽

pp. 340-346 ◽

Cited By ~ 5

Author(s):

Rajesh M. Kashid ◽

Santosh G. Singh ◽

Shrawan Singh

Keyword(s):

High Performance Liquid Chromatography ◽

Simultaneous Determination ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Hplc Method ◽

Reversed Phase Hplc ◽

Methyl Paraben ◽

Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

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