Systematic procedure for the determination of the nature of the solutes prior to the selection of the mobile phase parameters for optimization of reversed-phase ion-pair chromatographic separations

1989 ◽  
Vol 478 (1) ◽  
pp. 21-38 ◽  
Author(s):  
G Low
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Ion Pair ◽  
Chromatographic Separations ◽  
Systematic Procedure ◽  
Selection Of

scholarly journals Rapid Determination of Thiamine, Riboflavin, Pyridoxine, and Niacinamide in Infant Formulas by Liquid Chromatography

2002 ◽  
Vol 85 (4) ◽  
pp. 945-951 ◽  
Author(s):  
David C Woollard ◽  
Harvey E Indyk
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
Rapid Determination ◽  
Reversed Phase ◽  
Ion Pair ◽  
Infant Formulas ◽  
Ion Pair Chromatography ◽  
Naturally Occurring ◽  
Sample Extract

Abstract A simplified, simultaneous determination of vitamins B1, B2, B3, and B6 in supplemented infant formulas was developed from a single deproteinized sample extract, with analysis by reversed-phase, ion-pair chromatography with an acidified methanol–water mobile phase. The dioctylsulfosuccinate counter-ion facilitates unique retention of the pyridine-based vitamins (niacinamide and pyridoxine) and allows for concurrent measurement of both the pyridoxal and riboflavin 5′-phosphate endogenous components of milk. Other naturally occurring undetected vitamin congeners have minimal analytical significance. UV detection is used for niacinamide, and programmed fluorescence detection is used for riboflavin and the B6 vitamins. Thiamine is routinely determined sequentially under modified elution conditions.

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

1994 ◽  
Vol 59 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Josef Královský ◽  
Marta Kalhousová ◽  
Petr Šlosar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Optimum Conditions ◽  
Linear Calibration ◽  
Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

Simultaneous Determination of Ajmaline and 17-Monochloroacetylajmaline by Ion-Pair Reversed-Phase HPCL

1984 ◽  
Vol 17 (5) ◽  
pp. 423-431 ◽  
Author(s):  
L. Gagliardi ◽  
G. Cavazzutti ◽  
A. Amato ◽  
V. Zagarese ◽  
F. Chimenit ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Reversed Phase ◽  
Ion Pair
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