Monitoring of new silica-based reversed-phase stationary phases for the liquid chromatographic analysis of basic pharmaceuticals using principal components analysis

1997 ◽  
Vol 765 (2) ◽  
pp. 157-168 ◽  
Author(s):  
R.J.M. Vervoort ◽  
M.W.J. Derksen ◽  
A.J.J. Debets
Keyword(s):  
Principal Components Analysis ◽  
Principal Components ◽  
Chromatographic Analysis ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Liquid Chromatographic Analysis ◽  
Components Analysis ◽  
Liquid Chromatographic

Application of "high-performance" liquid chromatography to the study of sphingolipidoses.

1980 ◽  
Vol 26 (10) ◽  
pp. 1499-1503 ◽  
Author(s):  
M D Ullman ◽  
R E Pyeritz ◽  
H W Moser ◽  
D A Wenger ◽  
E H Kolodny
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Plasma Exchange ◽  
Chromatographic Analysis ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Urine Sediment ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract Quantitative high-performance liquid chromatographic analysis of perbenzoylated sphingolipids has been used to study the correlations of body chemistry to clinical phenomena. Plasma sphingolipids were isolated from 32 Gaucher (β-glucosidase deficiency) and six Fabry (α-galactosidase deficiency) patients by solvent partition and chromatographic separation on silicic acid columns. Plasma sphingolipids from a patient undergoing plasma-exchange were separated from interfering lipids with reversed-phase columns. Liquid-chromatographic analysis of sphingolipids provides useful supportive information for diagnoses because affected individuals are shown to possess increased circulating concentrations of the pathognomonic sphingolipid. We also used this technique to monitor sphingolipid concentrations in plasma and urine sediment during plasma exchange of a p atient with Fabry’s disease. Regular plasma exchanges produced and maintained decreased concentrations of sphingolipids in plasma, but near pre-exchange concentrations were observed within days after the therapy was terminated.

Liquid-chromatographic analysis for serum theophylline in less than 70 seconds.

1982 ◽  
Vol 28 (4) ◽  
pp. 687-689 ◽  
Author(s):  
P M Kabra ◽  
L J Marton
Keyword(s):  
Chromatographic Analysis ◽  
High Speed ◽  
Limit Of Detection ◽  
Flow Cell ◽  
Reversed Phase ◽  
Serum Theophylline ◽  
Analytical Recovery ◽  
Micro Flow ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a sensitive, specific, and very fast liquid-chromatographic assay for serum theophylline, involving a commercially available high-speed reversed-phase column and a micro-flow-cell-equipped detector. Each analysis requires only 100 microL of serum (as little as 25 microL may be used when necessary), and chromatography is complete in less than 70 s. Analytical recovery of theophylline added to serum ranged from 97 to 102%. Between-run precision (CV) ranged from 2.1 to 3.5%. The lower limit of detection for theophylline is 0.5 mg/L, and linearity extends to 50 mg/L. Numerous drugs and xanthine metabolites tested do not interfere.

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