Rapid determination of glyphosate in cereal samples by means of pre-column derivatisation with 9-fluorenylmethyl chloroformate and coupled-column liquid chromatography with fluorescence detection

1999 ◽  
Vol 833 (1) ◽  
pp. 67-73 ◽  
Author(s):  
E.A. Hogendoorn ◽  
F.M. Ossendrijver ◽  
E. Dijkman ◽  
R.A. Baumann
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Rapid Determination ◽  
Cereal Samples

scholarly journals Rapid Determination of Fluoroquinolone Residues in Honey by a Microbiological Screening Method and Liquid Chromatography

2010 ◽  
Vol 93 (4) ◽  
pp. 1331-1339 ◽  
Author(s):  
Maki Kanda ◽  
Tomoto Kusano ◽  
Setsuko Kanai ◽  
Hiroshi Hayashi ◽  
Yoko Matushima ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Efficient Method ◽  
Rapid Determination ◽  
Screening Method ◽  
Microbiological Method ◽  
Metal Free ◽  
Spe Method

Abstract A rapid and efficient method was developed for the simultaneous determination of seven fluoroquinolone (FQ) residues: norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin, and difloxacin in honey. The samples were first screened with a microbiological method by using test plates made from metal-free purified agar seeded with Bacillus subtilis BGA. When a sample was found to contain FQ residues by using the microbiological method, it was analyzed by LC with fluorescence detection (LC/FL). FQs were extracted with Na2EDTA-McIlvaine buffer and purified by a dual SPE method in which a cation-exchange cartridge was connected to an anion-exchange cartridge. The overall recoveries of the seven FQs ranged from 70.0 to 92.1. The intra-assay and interassay CVs were 7.8 and 5.1, respectively. For the microbiological method, the LOD values ranged from 2 to 9 g/kg. For LC/FL, the LOQ values ranged from 2 to 7 g/kg. The developed method was used to analyze 70 honey samples. In 14 samples in which the microbiological method detected the presence of FQ residues, norfloxacin, ciprofloxacin, and enrofloxacin were identified by LC/FL.

scholarly journals Rapid Determination of Nosiheptide in Meat and Egg by Liquid Chromatography with Fluorescence Detection

2000 ◽  
Vol 83 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Shozo Horii ◽  
Naoto Oku
Keyword(s):  
Liquid Chromatography ◽  
Phosphoric Acid ◽  
Fluorescence Detection ◽  
Mobile Phase ◽  
Lower Layer ◽  
Rapid Determination ◽  
Chicken Muscle

Abstract A procedure was developed to determine nosiheptide residues in marketed meat and egg. Acetonitrile was used for the extraction, and the extract was partitioned with hexane to remove fat. The lower layer was reconstructed and quantitated by liquid chromatography using fluorescence detection at 357 nm excitation and 500 nm emission. The mobile phase consisted of 0.025% phosphoric acid–acetonitrile (50 + 50, v/v). Recoveries of nosiheptide from fortified samples ranged from 91.3 to 95.2% for swine muscle, 88.6 to 92.7% for chicken muscle, and 86.3 to 86.8% for egg. The method was used to monitor swine and chicken muscle and egg (20 samples each) in the market. Nosiheptide was not determined in all 60 samples.

Determination of five beta-blockers in wastewaters by coupled-column liquid chromatography and fluorescence detection

2010 ◽  
Vol 666 (1-2) ◽  
pp. 38-44 ◽  
Author(s):  
P. Parrilla Vázquez ◽  
M. Martínez Galera ◽  
A. Serrano Guirado ◽  
M.M. Parrilla Vázquez
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Beta Blockers

scholarly journals Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Grains by New Immunoaffinity Column/Liquid Chromatography

2004 ◽  
Vol 87 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Roswitha Göbel ◽  
Klaus Lusky
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Trifluoroacetic Acid ◽  
Detection Limits ◽  
Immunoaffinity Column ◽  
Recovery Rates

Abstract The simultaneous determination of mycotoxins was performed in 3 steps: extraction, cleanup, and detection. For extraction, a mixture of acetonitrile–water (60 + 40, v/v) was proved appropriate. For cleanup, a new Afla-Ochra-Zea immunoaffinity column was used. After derivatization with trifluoroacetic acid, the mycotoxins aflatoxins, ochratoxin A (OTA), and zearalenone (ZEA) were determined simultaneously by liquid chromatography with fluorescence detection. The detection limits in different matrixes after cleanup with the new immunoaffinity column were very low: aflatoxins, 0.002–0.7 μg/kg; OTA, 0.07–0.25 μg/kg; ZEA, 1–3 μg/kg. The limits of determination were: aflatoxins, 0.25 μg/kg; OTA, 0.5 μg/kg; ZEA, 5 μg/kg. The recovery rates for aflatoxins, OTA, and ZEA for rye and rice were between 86 and 93% when a 0.5 g sample matter per immunoaffinity column was used.

Sign in / Sign up

Export Citation Format

Share Document