Determination of ochratoxin A in wine by means of immunoaffinity column clean-up and high-performance liquid chromatography

1999 ◽  
Vol 864 (1) ◽  
pp. 89-101 ◽  
Author(s):  
Angelo Visconti ◽  
Michelangelo Pascale ◽  
Gianluca Centonze
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ochratoxin A ◽  
High Performance ◽  
Immunoaffinity Column

Determination of Ochratoxin A in Pig Kidneys by Immunoaffinity Cleanup and Ultra-High Performance Liquid Chromatography

2015 ◽  
Vol 98 (6) ◽  
pp. 1566-1570 ◽  
Author(s):  
YaLi Hou ◽  
Jinhui Zhou ◽  
YuPing Li ◽  
JiangHua Xie ◽  
Li Zhou ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ochratoxin A ◽  
High Performance ◽  
Accurate Method ◽  
Immunoaffinity Column ◽  
Trace Level ◽  
Pig Kidney ◽  
Identification And Quantification

Abstract An accurate method was developed for determining ochratoxin A (OTA) in pig kidney using an immunoaffinity column for cleanup and ultra-HPLC/MS/MS for identification and quantification. Mean recoveries of OTA from kidney samples fortified at 0.10–5 μg/kg levels ranged from 74 to 92%, with RSDs of 4.6–7.5%. The LOD was estimated to be 0.03 μg/kg and the LOQ was 0.10 μg/kg based on an S/N in kidney of 3:1 and 10:1, respectively. The developed method was used for the determination of OTA in pig kidney. A survey of the OTA content of pig kidneys slaughtered in Chongqing, China, revealed that 35 out of 40 analyzed samples were contaminated; the OTA concentration in kidney ranged from trace level (0.03–0.1) to 0.323 μg/kg. This method was shown to be useful for accurately evaluating the intake of OTA from pig kidneys.

scholarly journals Determination of aflatoxins and ochratoxin A in wheat from different regions of Turkey by HPLC with fluorescence detection

2020 ◽  
Vol 49 (1) ◽  
pp. 118-124
Author(s):  
S. Turksoy ◽  
B. Kabak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Food Safety ◽  
Ochratoxin A ◽  
High Performance ◽  
Liquid Extraction ◽  
Immunoaffinity Column ◽  
Contamination Levels ◽  
Aflatoxin B

This study examines the occurrence of aflatoxins (AFS) and ochratoxin A (OTA) in bread and durum wheat samples. A total of 141 samples were collected from eleven different regions of Turkey. An analytical method based on liquid extraction, immunoaffinity column (IAC) clean-up followed by high performance liquid chromatography (HPLC) was used for the determination of AFs and OTA levels. As a result, AFs and OTA were detected in 2% and 9.2% of wheat samples at concentrations varying from 0.21 to 0.44 µg kg−1 and from 0.1 to 3.2 µg kg−1, respectively. Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) were found positive in samples ranging between 0.21–0.35 µg kg−1 and 0.094 µg kg−1, respectively. However, none of the samples contained aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2). The study also recommended that contamination levels in wheat and wheat-based products should be routinely monitored in greater sample numbers to insure food safety.

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