scholarly journals Effect of chain length on rates of uptake of free fatty acids during in vitro incubations of rat adipose tissue

1965 ◽  
Vol 6 (4) ◽  
pp. 565-571
Author(s):  
Jerome L. Knittle ◽  
Jules Hirsch
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Chain Length ◽  
Rat Adipose Tissue

Relation of fatty acid structure to release and esterification of free fatty acids

1963 ◽  
Vol 205 (5) ◽  
pp. 909-912 ◽  
Author(s):  
C. H. Hollenberg ◽  
A. Angel
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Chain Length ◽  
Binding Sites ◽  
Free Acid ◽  
Aqueous Solubility ◽  
Subsequent Exposure ◽  
Rat Adipose Tissue ◽  
Acid Structure

After incubation of rat adipose tissue with corticotrophin in buffer-albumin, the composition of the free fatty acids (FFA) in the tissue and in the medium was determined; the medium contained proportionately more myristic, palmitoleic, linoleic, and linolenic acids. The ratio of the amount of each free acid in the medium to that in the tissue increased with decreasing chain length and increasing unsaturation. Free acids produced in adipose tissue by corticotrophin stimulation were esterified by subsequent exposure of the tissue to glucose and insulin. Among the saturated and monoenoic acids the shorter chain components were esterified to a greater extent, while at a given chain length the more unsaturated acids were more readily esterified. Thus the more polar FFA were more readily released from and esterified in adipose tissue. These results could have been produced by a variety of processes including differences between the acids in aqueous solubility or the presence of tissue-binding sites with varying affinities for the different acids.

Effect of nicotine on the mobilization of free fatty acids from adipose tissue in vitro

1969 ◽  
Vol 25 (2) ◽  
pp. 128-128 ◽  
Author(s):  
A. Kershbaum ◽  
H. Osada ◽  
D. J. Pappajohn ◽  
S. Bellet
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Adipose Tissue In Vitro

THE STIMULATING EFFECTS OF ADRENALINE AND ANTERIOR PITUITARY HORMONES ON THE RELEASE OF FREE FATTY ACIDS FROM ADIPOSE TISSUE

1962 ◽  
Vol 25 (2) ◽  
pp. 189-198 ◽  
Author(s):  
R. M. BUCKLE
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Adrenocorticotrophic Hormone ◽  
Fat Pads

SUMMARY The quantity of free fatty acids (FFA) released from rat epididymal fat pads in vitro and their concentration within the tissue were determined. The addition of adrenaline, adrenocorticotrophic hormone (ACTH), thyroid stimulating hormone (TSH) and growth hormone (GH) each increased the release of FFA, and their respective minimum effective concentrations were 0·125, 0·004, 0·5 and 1·25 μg./ml. of medium. In every case, the increased release of FFA was associated with a rise in the quantity present within the pads, and the amount released closely paralleled their concentration within the tissue. It is suggested that the stimulatory effect of all four hormones on the release of FFA from adipose tissue is largely a manifestation of their activity of increasing the concentration of FFA within the cells, and this they do by facilitating the net conversion of storage triglyceride to fatty acid. The significance of the relative activities of the hormones in vitro is discussed and compared with their fatty acid mobilizing effects in vivo.

scholarly journals Fatty acids synthesis in rat adipose tissue. Tracer concentration effects in vitro

1968 ◽  
Vol 106 (1) ◽  
pp. 179-183 ◽  
Author(s):  
W. G. Duncombe
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Acid Synthesis ◽  
Long Chain Fatty Acids ◽  
Concentration Effects ◽  
Tracer Concentration ◽  
Adipose Tissue In Vitro ◽  
Rat Adipose Tissue ◽  
Long Chain

1. The use of labelled acetate for studying the synthesis of long-chain fatty acids in rat adipose tissue in vitro has been examined, with special reference to the effect of acetate concentration. 2. The incorporation of acetate into fatty acids is proportional to the concentration of acetate in the medium when the latter does not exceed about 10μm. Above this concentration, the relative incorporation becomes progressively less, and reasons for this are discussed. 3. In particular it is shown that this is not necessarily due to disturbance of the endogenous rate of fatty acid synthesis by a relatively large amount of acetyl-CoA derived from added acetate. 4. However, to ensure that the added acetate does not cause such a disturbance its concentration must be kept sufficiently low. For labelled acetate used under present conditions, this concentration should not be more than about 10μm.

Effect of β-adrenergic agonists on lipolysis and lipogenesis by porcine adipose tissue in vitro

1990 ◽  
Vol 68 (4) ◽  
pp. 1024-1029 ◽  
Author(s):  
T. A. Peterla ◽  
C. G. Scanes
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Direct Effects ◽  
Adrenergic Agonists ◽  
Tissue Explants ◽  
In Vitro Lipolysis ◽  
Adipose Tissue In Vitro ◽  
Release Of Glycerol

Abstract The effects of the β-adrenergic agonists isoproterenol, cimaterol, ractopamine and clenbuterol on lipolysis (release of glycerol and free fatty acids) and lipogenesis (incorporation of 14C into fatty acids from [14C]glucose) was examined in porcine adipose tissue explants in vitro. Lipolysis was stimulated by isoproterenol, cimaterol or ractopamine but not by clenbuterol. Insulin reduced the lipolytic effects of the β-adrenergic agonists (isoproterenol, cimaterol and ractopamine). Lipogenesis was inhibited by all β-adrenergic agonists tested (isoproterenol, cimaterol, ractopamine and clenbuterol). The antilipogenic effect of the β-adrenergic agonists was reduced by the presence of insulin in the incubation. Although effects of the different β-adrenergic agonists varied, all had some direct effects that could be expected to reduce adipose accretion. Effects of β-adrenergic agonists in the pig are due in part to direct effects on adipose tissue.

Effect of pyridinolcarbamate on rat adipose tissue free fatty acids

1972 ◽  
Vol 16 (2) ◽  
pp. 185-191 ◽  
Author(s):  
D Grafnetter ◽  
T Shimamoto ◽  
F Numano
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Free Fatty Acids ◽  
Rat Adipose Tissue
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