Identification of tissue-specific and developmental stage-specific nuclear proteins that bind to two elements in the 5′-flanking region of the human myoglobin gene

1987 ◽  
Vol 19 ◽  
pp. S22-S22
Author(s):  
W KRAUS ◽  
M GARCIAMOLL ◽  
R SANDERSWILLIAMS
Keyword(s):  
Developmental Stage ◽  
Nuclear Proteins ◽  
Tissue Specific ◽  
Human Myoglobin ◽  
Flanking Region

scholarly journals Tissue-specific interactions between nuclear proteins and the aminopeptidase N promoter.

1991 ◽  
Vol 266 (27) ◽  
pp. 18089-18096
Author(s):  
J. Olsen ◽  
L. Laustsen ◽  
U. Kärnström ◽  
H. Sjöström ◽  
O. Norén
Keyword(s):  
Aminopeptidase N ◽  
Nuclear Proteins ◽  
Specific Interactions ◽  
Tissue Specific

scholarly journals Identification of a Muscle-specific Enhancer within the 5′-Flanking Region of the Human Myoglobin Gene

1989 ◽  
Vol 264 (23) ◽  
pp. 13896-13901 ◽  
Author(s):  
B H Devlin ◽  
F C Wefald ◽  
W E Kraus ◽  
T S Bernard ◽  
R S Williams
Keyword(s):  
Human Myoglobin ◽  
Flanking Region

scholarly journals Tissue-specific expression of apolipoprotein A-I (ApoA-I) is regulated by the 5′-flanking region of the human ApoA-I gene.

1988 ◽  
Vol 263 (34) ◽  
pp. 18530-18536 ◽  
Author(s):  
K Higuchi ◽  
S W Law ◽  
J M Hoeg ◽  
U K Schumacher ◽  
N Meglin ◽  
...  
Keyword(s):  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Apolipoprotein A ◽  
Flanking Region ◽  
I Gene

Cooperativity of sequence elements mediates tissue specificity of the rat insulin II gene

1990 ◽  
Vol 10 (4) ◽  
pp. 1784-1788
Author(s):  
Y P Hwung ◽  
Y Z Gu ◽  
M J Tsai
Keyword(s):  
Beta Cell ◽  
Tissue Specificity ◽  
Promoter Element ◽  
Heterologous Promoter ◽  
Specific Expression ◽  
Tissue Specific ◽  
Cis Acting ◽  
Insulin Promoter ◽  
Sequence Elements ◽  
Flanking Region

The 5'-flanking region of the rat insulin II gene (-448 to +50) is sufficient for tissue-specific expression. To further determine the tissue-specific cis-acting element(s), important sequences defined by linker-scanning mutagenesis were placed upstream of a heterologous promoter and transfected into insulin-producing and -nonproducing cells. Rat insulin promoter element 3 (RIPE3), which spans from -125 to -86, was shown to confer beta-cell-specific expression in either orientation. However, two subregions of RIPE3, RIPE3a and RIPE3b (defined by linker-scanning mutations), displayed only marginal activities. These results suggest that the two subregions cooperate to confer tissue specificity, presumably via their cognate binding factors.

The human beta fibrinogen promoter contains a hepatocyte nuclear factor 1-dependent interleukin-6-responsive element

1993 ◽  
Vol 13 (2) ◽  
pp. 1183-1193
Author(s):  
J Dalmon ◽  
M Laurent ◽  
G Courtois
Keyword(s):  
Binding Site ◽  
Acute Phase ◽  
Interleukin 6 ◽  
Constitutive Expression ◽  
Nuclear Proteins ◽  
Functional Coupling ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Tissue Specific ◽  
Nuclear Factor 1

Acute-phase reactants are liver proteins whose synthesis is positively or negatively regulated during inflammation. The main mediators of this phenomenon are glucocorticoids and interleukin-6 (IL-6), a pleiotropic cytokine that also controls hematopoiesis. Functional analysis of several acute-phase reactant promoter regions has identified two major DNA motifs used by IL-6-regulated genes. The first one corresponds to a CTGG(G/A)AA sequence, and the other is a binding site for members of the C/EBP family of nuclear proteins. We have previously shown that the human beta fibrinogen (beta Fg) promoter contains an IL-6-responsive region, located between bp -150 and -67 (P. Huber, M. Laurent, and J. Dalmon, J. Biol. Chem. 265:5695-5701, 1990). In this study, using DNase I footprinting, mobility shift assays, and mutagenesis, we demonstrate that at least three subdomains of this region are necessary to observe a full response to IL-6. The most distal contains a CTGGGAA motif, and its mutation inhibits IL-6 stimulation. Another, which is able to interact with several distinct nuclear proteins, among them members of the C/EBP family, is dispensable for IL-6 induction but plays an important role in the constitutive expression of beta Fg. Finally, a proximal hepatocyte nuclear factor 1 binding site, already described as the major determinant of beta Fg tissue-specific expression, is also required for IL-6 stimulation. These results indicate a complex interplay between nuclear proteins within the beta Fg IL-6-responsive region and suggest a tight functional coupling between the tissue-specific and inducible elements.

scholarly journals Cooperativity of sequence elements mediates tissue specificity of the rat insulin II gene.

1990 ◽  
Vol 10 (4) ◽  
pp. 1784-1788 ◽  
Author(s):  
Y P Hwung ◽  
Y Z Gu ◽  
M J Tsai
Keyword(s):  
Beta Cell ◽  
Tissue Specificity ◽  
Promoter Element ◽  
Heterologous Promoter ◽  
Specific Expression ◽  
Tissue Specific ◽  
Cis Acting ◽  
Insulin Promoter ◽  
Sequence Elements ◽  
Flanking Region

The 5'-flanking region of the rat insulin II gene (-448 to +50) is sufficient for tissue-specific expression. To further determine the tissue-specific cis-acting element(s), important sequences defined by linker-scanning mutagenesis were placed upstream of a heterologous promoter and transfected into insulin-producing and -nonproducing cells. Rat insulin promoter element 3 (RIPE3), which spans from -125 to -86, was shown to confer beta-cell-specific expression in either orientation. However, two subregions of RIPE3, RIPE3a and RIPE3b (defined by linker-scanning mutations), displayed only marginal activities. These results suggest that the two subregions cooperate to confer tissue specificity, presumably via their cognate binding factors.

The melanin-concentrating hormone gene in human: flanking region analysis, fine chromosome mapping, and tissue-specific expression

1997 ◽  
Vol 46 (1-2) ◽  
pp. 243-255 ◽  
Author(s):  
Agnès Viale ◽  
Yao Zhixing ◽  
Christophe Breton ◽  
Florence Pedeutour ◽  
Antoine Coquerel ◽  
...  
Keyword(s):  
Chromosome Mapping ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Region Analysis ◽  
Melanin Concentrating Hormone ◽  
Flanking Region

scholarly journals Sonic hedgehog is required for neural crest-dependent patterning of the intrinsic tongue musculature

2017 ◽  
Author(s):  
Shigeru Okuhara ◽  
Anahid A. Birjandi ◽  
Hadeel Adel Al-Lami ◽  
Tomoko Sagai ◽  
Takanori Amano ◽  
...  
Keyword(s):  
Neural Crest ◽  
Developmental Stage ◽  
Sonic Hedgehog ◽  
Functional Requirement ◽  
Cranial Neural Crest ◽  
Tissue Specific ◽  
Specific Loss ◽  
Dorsal Tongue ◽  
Tongue Muscles

AbstractThe tongue is a highly specialized muscular organ important for breathing, speech, taste and swallowing. The secreted signaling molecule Sonic hedgehog (Shh) is expressed in dorsal tongue epithelium from the initial developmental stage. In this study, we utilized a series of genetic approaches to investigate the role of Shh during mouse tongue formation. Temporal-specific global deletion of Shh demonstrated a functional requirement for normal patterning of the intrinsic tongue muscles and establishment of the lingual tendon. These defects were reproduced in the mutant with a specific loss of signaling in oropharyngeal epithelium by a Shh cis-enhancer. In these mutants, Ptch1 was lost in the underlying cranial neural crest (CNC)-derived mesenchymal lineage. The importance of Shh was confirmed by generating tissue-specific deletions in the ciliopathy gene Ofd1, which transduces Shh signaling. These results revealed Shh roles in patterning of the mesodermal intrinsic tongue muscles through CNC-derived mesenchyme, including the lingual tendon.

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