A technique for radiographic cannulation and opacification of anatomical structures with small orifices—A new method

The wide spectrum of variations in the types of cleft lip and cleft palate has made standardized and inclusive classification difficult. This article introduces a new method for recording all types of cleft lip and cleft palate for data storage and communication. The proposed Expression System incorporates the actual words for the anatomical structures affected by clefts and can describe in a quickly understood form, without the need to consult reference materials, the location and extent of both typical and atypical clefts. The Expression System overcomes several limitations of previous cleft registration methods, and its simplicity and precision will benefit dental and medical specialists by furthering inter- and intradisciplinary communication.

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Automatic Localization of the Optic Disc Center in Retinal Images Based on Angle Detection in Curvature Scale Space

Medical Imaging ◽

10.4018/978-1-5225-0571-6.ch026 ◽

2017 ◽

pp. 679-692

Author(s):

A. Elbalaoui ◽

Mohamed Fakir ◽

M. Boutaounte ◽

A. Merbouha

Keyword(s):

Diabetic Retinopathy ◽

Optic Disc ◽

Digital Images ◽

Scale Space ◽

New Method ◽

Retinal Images ◽

Anatomical Structures ◽

Disc Center ◽

Curvature Scale Space ◽

Automatic Localization

Digital images of the retina is widely used for screening of patients suffering from sight threatening diseases such as Diabetic retinopathy and Glaucoma. The localization of the Optic Disc (OD) center is the first and necessary step identification and segmentation of anatomical structures and in pathological retinal images. From the center of the optic disc spreads the major blood vessels of the retina. Therefore, by considering the high number of vessels and the high number of the angles resulted from the vessels crossing, the authors propose a new method based on the number of angles in the vicinity of optic disc for localization of the center of optic disc. The first step is pre-processing of retinal image for separate the fundus from its background and increase the contrast between contours. In the second step, the authors use the Curvature Scale Space (CSS) for angle detection. In the next step, they move a window about the size of optic disc to count the number of corners. In the final step, they use the center of windows which has the most number of corners for localizing the optic disc center. The proposed method is evaluated on DRIVE, CHASE_DB1 and STARE databases and the success rate is 100, 100 and 96.3%, respectively.

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Topographic-Anatomical Validation of a New Method for Minimally-Invasive Extra-Cortical Osteosynthesis Using Plastinated Transverse Shoulder Cuts

Journal of Anatomy and Histopathology ◽

10.18499/2225-7357-2020-9-1-49-55 ◽

2020 ◽

Vol 9 (1) ◽

pp. 49-55

Author(s):

A. Yu. Kochish ◽

D. A. Starchik ◽

B. A. Maiorov ◽

I. G. Belen'kii

Keyword(s):

Peripheral Nerves ◽

New Method ◽

Anatomical Structures ◽

Anatomical Studies ◽

Metal Implants ◽

Shoulder Muscle ◽

Metal Plates ◽

Transparent Plate ◽

Long Head ◽

The Right

The aim of the study was to assess capability and benefits of this method, its technical feasibility and safety regarding a damage of the most essential anatomical structures.Material and methods. The study involved 15 natural humeral bones; their parameters - the length and shape of the plates – were measured for osteosynthesis. Bone osteosynthesis was further performed on 14 intact upper limbs taken from 7 non-fixed corpses of people died at the age of 63–77 using the discussed method; after that, the operated limbs were studied using preparating technique. Furthermore, after plate application on two shoulder sections, 12 transverse cuts with their epoxy plastination were made at three levels according to the original technology; this was followed by the preparation of histotopographic sections.Results. The results obtained have demonstrated that for the new method of bone osteosynthesis it is advisable to use metal plates 220–260 mm long with spiral twisting clockwise for the right humerus and counterclockwise for the left. Average distances from the fixed plate to the humerus, tendon of the long head of the biceps of the shoulder muscle, large blood vessels and peripheral nerves were determined using precision preparation and measurements; the measurements were also performed on transparent plate-shaped shoulder cuts. All the above validates safety of the discussed method regarding a damage of the most essential anatomical structures and allow to successfully use it in the clinical practice.Conclusion. The discussed technique of plastinated transverse shoulder cuts with further experimental fixation of metal implants for osteosynthesis ensures safety of the initial topographic and anatomical relationships of these structures with anatomical structures. In addition, high transparency of the tissues created by epoxy plastination allows determining distances between implants and important anatomical structures with high precision, the fact being of great significance in applied anatomical studies.

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Selection and Preparation of Specific Tissue Regions For Tem Using Large Epoxy-Embedded Blocks

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010007206x ◽

1973 ◽

Vol 31 ◽

pp. 324-325 ◽

Cited By ~ 1

Author(s):

P. M. Lowrie ◽

W. S. Tyler

Keyword(s):

Electron Microscopy ◽

Anatomical Structures ◽

Ultrastructural Studies ◽

Transmission Electron ◽

Microscopic Evaluation ◽

Embedded Blocks ◽

Specific Tissue ◽

Definition Of ◽

Areas Of Interest ◽

Selection Of

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).

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Selective Sampling and Orientation of Non-Homogeneous Tissues

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100238 ◽

1968 ◽

Vol 26 ◽

pp. 98-99

Author(s):

C. C. Clawson ◽

L. W. Anderson ◽

R. A. Good

Keyword(s):

Electron Microscopy ◽

Electron Microscope ◽

Light Microscopy ◽

Random Sampling ◽

New Method ◽

Microscope Examination ◽

Small Areas ◽

Selective Sampling ◽

Large Numbers ◽

Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

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