Red cell metabolism in iron deficiency anemia. II. The relationship between red cell survival and alterations in red cell metabolism

1970 ◽  
Vol 76 (5) ◽  
pp. 660-675 ◽  
Author(s):  
Lorna G. Macdougall ◽  
Janifer M. Judisch ◽  
Silloo B. Mistry
Keyword(s):  
Iron Deficiency ◽  
Cell Survival ◽  
Iron Deficiency Anemia ◽  
Cell Metabolism ◽  
Deficiency Anemia ◽  
Red Cell ◽  
Red Cell Metabolism ◽  
Red Cell Survival ◽  
The Relationship

scholarly journals Excess Hemolysis in Subjects with Severe Iron Deficiency Anemia Associated and Nonassociated with Hookworm Infection

Blood ◽  
1965 ◽  
Vol 25 (1) ◽  
pp. 73-91 ◽  
Author(s):  
MIGUEL LAYRISSE ◽  
JESÚS LINARES ◽  
MARCEL ROCHE ◽  
Adelina Ojeda ◽  
Alvaro Carstens ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Cell Survival ◽  
Iron Deficiency Anemia ◽  
Red Cells ◽  
Deficiency Anemia ◽  
Intrinsic Defect ◽  
Red Cell ◽  
Hookworm Infection ◽  
Iron Treatment ◽  
Red Cell Survival

Abstract An excess hemolysis was found in subjects with iron deficiency anemia associated with hookworm infection. Red cell survival, measured with Cr51 and DFP32 in the subjects before deworming, showed a marked disproportion between the decrease of the survival and the amount of daily intestinal blood loss in most cases. Excess of hemolysis was still present after more than 90 per cent of the parasites were removed. Red cell survival became normal after correction of anemia through iron treatment. Excess of hemolysis was also present in noninfected subjects with iron deficiency anemia due to other causes. The reduction in the survival of the erythrocytes from infected subjects transfused into normal recipients shows that the hemolytic process is due to an intrinsic defect of the red cells. The low values of hemoglobinemia and the presence of haptoglobins in the plasma indicate that hemoglobin has not been liberated in excess intravascularly. Finally, the fact that the red cells from an infected patient taken after deworming survived normally in splenectomized recipients indicates that the spleen is probably the principal site of the red cell destruction. The clinical and autopsy findings suggest that splenic function is not pathologically increased, but rather that this organ is acting physiologically at a more rapid rate, "culling" the abnormal circulating red cells and thus leading to a decrease in red cell survival. The studies presented here also indicate that the hookworm infection per se does not induce hemolysis.

scholarly journals The Dpg gene: an intracorpuscular modifier of red cell metabolism

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1210-1214 ◽  
Author(s):  
NA Noble ◽  
G Rothstein
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Cell Metabolism ◽  
Genetic Locus ◽  
Red Cell ◽  
Red Cell Metabolism ◽  
Red Cell Survival ◽  
Long Evans ◽  
Total Body

Abstract The genetic locus designated Dpg has two alleles in outbred Long-Evans rats. Genotype at this locus affects quantities of red cell 2,3- diphosphoglycerate (DPG) and adenosine triphosphate, as well as activities of two important glycolytic enzymes: phosphofructokinase and pyruvate kinase. Intravascular red cell survival is shortened in low- DPG animals. In order to get closer to the specific action of this locus, we addressed the question of whether the Dpg gene acts through intracorpuscular or extracorpuscular factors. Bone marrow transplantation after total body irradiation and 51Cr red cell survival after cross transfusion were the methods used. Because the animals that were used differed in hemoglobin phenotype, donor and recipient cells could be quantified in cross-transplanted animals. Phenotypic markers of Dpg genotype were measured in animals 40 to 50 days after transplantation. Values for these markers correlated highly with the percentage of donor and recipient cells present. In vivo survival of low-DPG red cells was significantly shorter than that of high-DPG cells (P less than .05), regardless of the genotype of the recipient. From the present studies, we conclude that the action of the Dpg gene is exerted by an intracorpuscular factor.

scholarly journals Mechanisms of Hemolysis in Iron Deficiency Anemia. Further Studies

Blood ◽  
1968 ◽  
Vol 32 (6) ◽  
pp. 884-894 ◽  
Author(s):  
MARIA DIEZ-EWALD ◽  
MIGUEL LAYRISSE ◽  
Adelina Ojeda
Keyword(s):  
Iron Deficiency ◽  
Cell Survival ◽  
Iron Deficiency Anemia ◽  
Red Cells ◽  
Deficiency Anemia ◽  
Intrinsic Defect ◽  
Red Cell ◽  
Cell Parameters ◽  
Survival Studies ◽  
Hook Worm

Abstract Red blood cell survival time as measured with 32DFP and 51Cr was studied in subjects with iron deficiency anemia associated and nonassociated with hook-worm infection. 51Cr T ½ was definitely shorter in all cases in which 32DFP showed marked excess hemolysis, but a normal 51Cr T ½ was found in some cases in which 32DFP survival was slightly reduced. There was a high correlation between survival measured with 32DFP and the rate of abnormal red cells in the peripheral blood. Less correlation was found between the proportion of hypochromic microcytic red cells and 32DFP, and even less correlation, although significant, was observed when 51Cr T ½ was compared with the red cell parameters. Results of the red cell survival studies with erythrocytes from subjects with iron deficiency anemia injected into the donor subject, normal recipients, and splenectomized subjects, as well as body 51Cr surface countings, indicated that excess hemolysis in this type of anemia is due to an intrinsic defect of the red blood cells and that the spleen is the principal sites of destruction of these cells.

scholarly journals The Dpg gene: an intracorpuscular modifier of red cell metabolism

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1210-1214
Author(s):  
NA Noble ◽  
G Rothstein
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Cell Metabolism ◽  
Genetic Locus ◽  
Red Cell ◽  
Red Cell Metabolism ◽  
Red Cell Survival ◽  
Long Evans ◽  
Total Body

The genetic locus designated Dpg has two alleles in outbred Long-Evans rats. Genotype at this locus affects quantities of red cell 2,3- diphosphoglycerate (DPG) and adenosine triphosphate, as well as activities of two important glycolytic enzymes: phosphofructokinase and pyruvate kinase. Intravascular red cell survival is shortened in low- DPG animals. In order to get closer to the specific action of this locus, we addressed the question of whether the Dpg gene acts through intracorpuscular or extracorpuscular factors. Bone marrow transplantation after total body irradiation and 51Cr red cell survival after cross transfusion were the methods used. Because the animals that were used differed in hemoglobin phenotype, donor and recipient cells could be quantified in cross-transplanted animals. Phenotypic markers of Dpg genotype were measured in animals 40 to 50 days after transplantation. Values for these markers correlated highly with the percentage of donor and recipient cells present. In vivo survival of low-DPG red cells was significantly shorter than that of high-DPG cells (P less than .05), regardless of the genotype of the recipient. From the present studies, we conclude that the action of the Dpg gene is exerted by an intracorpuscular factor.

scholarly journals Red Cell Distribution Width (RDW) and Hb% in the Detection of Iron Deficiency Anemia in Pregnant Women

2018 ◽  
Vol 9 (2) ◽  
pp. 137-141
Author(s):  
Gazi Sharmin Sultana ◽  
Syed Aminul Haque ◽  
Ayatunnesa ◽  
Md MA Muttalib ◽  
Md Quddusur Rahman
Keyword(s):  
Iron Deficiency ◽  
Pregnant Women ◽  
Iron Deficiency Anemia ◽  
Red Cell Distribution Width ◽  
Deficiency Anemia ◽  
Red Cell ◽  
Cell Distribution ◽  
Distribution Width ◽  
Latent Iron Deficiency ◽  
Automated Hematology Analyzer

Background: Detection of iron deficiency early during pregnancy is essential for correct management. Red cell distribution width (RDW) is a new routine parameter in fully automated hematology analyzer that can give the idea of early iron deficiency before Hb%. This study was aimed to see the role of red cell distribution width and Hb% in determining early iron deficiency in pregnant women.Methods: In this study 190 pregnant women were included. CBC including Hb% and RDW and iron profile were done. RDW were compared with Hb% in various stages of iron deficiency.Results: RDW was more significant than Hb level in latent iron deficiency when Hb level was normal (p<0.05). In mild and moderate iron deficiency anemia, RDW was increased progressively though Hb level was reduced. In this study RDW had sensitivity 82.3% and specificity 97.4%. Whereas Hb level had sensitivity 56.6% and specificity 90.9% for iron deficiency.Conclusion: Latent iron deficiency without other complicating disease could be screened out early by increased RDW when Hb% was normal.Anwer Khan Modern Medical College Journal Vol. 9, No. 2: Jul 2018, P 137-141

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