Plasmid copy number control: a case study of the quasi-steady-state assumption

1992 ◽  
Vol 158 (4) ◽  
pp. 481-494 ◽  
Author(s):  
Lee A. Segel ◽  
Alan S. Perelson
Keyword(s):  
Steady State ◽  
Copy Number ◽  
Plasmid Copy Number ◽  
Quasi Steady State ◽  
Plasmid Copy ◽  
Copy Number Control ◽  
Steady State Assumption ◽  
State Assumption

scholarly journals The Quasi-Steady-State Assumption: A Case Study in Perturbation

SIAM Review ◽  
1989 ◽  
Vol 31 (3) ◽  
pp. 446-477 ◽  
Author(s):  
Lee A. Segel ◽  
Marshall Slemrod
Keyword(s):  
Steady State ◽  
Quasi Steady State ◽  
Steady State Assumption ◽  
State Assumption

scholarly journals Plasmid copy number control: an ever-growing story

2002 ◽  
Vol 37 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Gloria Del Solar ◽  
Manuel Espinosa
Keyword(s):  
Copy Number ◽  
Plasmid Copy Number ◽  
Plasmid Copy ◽  
Copy Number Control

scholarly journals Genetic dissection of centromere function.

1993 ◽  
Vol 13 (6) ◽  
pp. 3156-3166 ◽  
Author(s):  
I G Schulman ◽  
K Bloom
Keyword(s):  
Chromosome Segregation ◽  
Copy Number ◽  
Artificial Chromosome ◽  
Plasmid Copy Number ◽  
Genetic Dissection ◽  
Negative Effects ◽  
Plasmid Copy ◽  
Complex Cells ◽  
Copy Number Control

A system to detect a minimal function of Saccharomyces cerevisiae centromeres in vivo has been developed. Centromere DNA mutants have been examined and found to be active in a plasmid copy number control assay in the absence of segregation. The experiments allow the identification of a minimal centromere unit, CDE III, independently of its ability to mediate chromosome segregation. Centromere-mediated plasmid copy number control correlates with the ability of CDE III to assemble a DNA-protein complex. Cells forced to maintain excess copies of CDE III exhibit increased loss of a nonessential artificial chromosome. Thus, segregationally impaired centromeres can have negative effects in trans on chromosome segregation. The use of a plasmid copy number control assay has allowed assembly steps preceding chromosome segregation to be defined.

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