scholarly journals Superiority of the University of Wisconsin solution over simple crystalloid for extended heart preservation

1992 ◽  
Vol 103 (5) ◽  
pp. 980-992 ◽  
Author(s):  
Wilson Ko ◽  
John A. Zelano ◽  
Richard Lazzaro ◽  
W. Douglas Lazenby ◽  
Thomas Hamilton ◽  
...  
Keyword(s):  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Heart Preservation ◽  
The University ◽  
Wisconsin Solution

scholarly journals Iodixanol Density Gradient Preparation in University of Wisconsin Solution for Porcine Islet Purification

2003 ◽  
Vol 3 ◽  
pp. 1154-1159 ◽  
Author(s):  
Michael P.M. Van der Burg ◽  
John M. Graham
Keyword(s):  
Adverse Effects ◽  
Human Islet ◽  
Porcine Pancreas ◽  
Isolated Pancreatic Islets ◽  
University Of Wisconsin Solution ◽  
Porcine Islet ◽  
University Of Wisconsin ◽  
Ficoll Gradient ◽  
The University ◽  
Wisconsin Solution

Previously published as Graham, J.M. (2002) Purification of Islets of Langerhans from porcine pancreas. TheScientificWorldJOURNAL 2, 1657–1661. ISSN 1537-744X; DOI 10.1100/tsw.2002.847.Generally, prior to the purification of isolated pancreatic islets, the collagenase-digested tissue is incubated in the University of Wisconsin solution (UWS; ~320 mOsm) for osmotic stabilization to preserve or improve the density differences between islets and acinar fragments. The adverse effects arising from the subsequent pelleting and resuspension of the islets in a second, different (often highly hyperosmotic) purification solution are avoided in the protocol described here; preparation of the purification medium is simply achieved by mixing the UWS preincubated islets with a second UWS containing the inert impermeant iodixanol. Flotation of the islets isolated from juvenile porcine pancreases through this mildly hypertonic (~380 mOsm) gradient of iodixanol-UWS achieves a much higher recovery of islets of an improved viability than the customary method using a Ficoll gradient. The method has been extended to human islet purification.

scholarly journals Cardiac transplantation after prolonged graft preservation with the University of Wisconsin solution

1992 ◽  
Vol 104 (2) ◽  
pp. 224-228 ◽  
Author(s):  
Valluvan Jeevanandam ◽  
Joseph S. Auteri ◽  
Juan A. Sanchez ◽  
Daphne Hsu ◽  
Charles Marboe ◽  
...  
Keyword(s):  
Cardiac Transplantation ◽  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Graft Preservation ◽  
The University ◽  
Wisconsin Solution

University of Wisconsin solution versus modified collins solution for canine heart preservation: An experimental study

2011 ◽  
Vol 6 (03) ◽  
pp. 176-179 ◽  
Author(s):  
Kazuhiro Sakata ◽  
Yoshiyuki Kawashima ◽  
Hideaki Ichikawa ◽  
Toshihiro Oya ◽  
Toru Takahashi ◽  
...  
Keyword(s):  
Experimental Study ◽  
Canine Heart ◽  
Solution Versus ◽  
University Of Wisconsin Solution ◽  
University Of Wisconsin ◽  
Heart Preservation ◽  
Wisconsin Solution
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