1004: In-Vivo Transfection of the Rat Corpus Cavernosum Using a Non-Viral, Linear Polyethylenimine Gene Delivery System: A Novel Application

The key strategy for the advancement of gene therapy is the development of an efficient targeted gene delivery system into cells. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relatively low transfection efficiency. It also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency. Also, the potential of clinical application was investigated.

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PEI-g-chitosan, a Novel Gene Delivery System with Transfection Efficiency Comparable to Polyethylenimine in Vitro and after Liver Administration in Vivo

Bioconjugate Chemistry ◽

10.1021/bc0501597 ◽

2006 ◽

Vol 17 (1) ◽

pp. 152-158 ◽

Cited By ~ 203

Author(s):

Kokhou Wong ◽

Guobin Sun ◽

Zhang ◽

Hui Dai ◽

Ye Liu ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Gene Delivery System ◽

Novel Gene

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Pancreatic Cancer Induced by In Vivo Electroporation-Enhanced Sleeping Beauty Transposon Gene Delivery System in Mouse

Pancreas ◽

10.1097/mpa.0000000000000102 ◽

2014 ◽

Vol 43 (4) ◽

pp. 614-618 ◽

Cited By ~ 7

Author(s):

June-Shine Park ◽

Kyung-Min Lim ◽

Sung Goo Park ◽

Sun Young Jung ◽

Hyun-Ji Choi ◽

...

Keyword(s):

Pancreatic Cancer ◽

Gene Delivery ◽

Delivery System ◽

Sleeping Beauty ◽

Gene Delivery System ◽

In Vivo Electroporation ◽

Sleeping Beauty Transposon

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Local Gene Delivery System by Bubble Liposomes and Ultrasound Exposure into Joint Synovium

Journal of Drug Delivery ◽

10.1155/2011/203986 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

Yoichi Negishi ◽

Yuka Tsunoda ◽

Yoko Endo-Takahashi ◽

Yusuke Oda ◽

Ryo Suzuki ◽

...

Keyword(s):

Gene Expression ◽

Gene Transfer ◽

Gene Delivery ◽

Delivery System ◽

Imaging System ◽

Effective Means ◽

Gene Delivery System ◽

Highly Efficient ◽

Efficient Gene

Recently, we have developed novel polyethylene glycol modified liposomes (bubble liposomes; BL) entrapping an ultrasound (US) imaging gas, which can work as a gene delivery tool with US exposure. In this study, we investigated the usefulness of US-mediated gene transfer systems with BL into synoviocytes in vitro and joint synovium in vivo. Highly efficient gene transfer could be achieved in the cultured primary synoviocytes transfected with the combination of BL and US exposure, compared to treatment with plasmid DNA (pDNA) alone, pDNA plus BL, or pDNA plus US. When BL was injected into the knee joints of mice, and US exposure was applied transcutaneously to the injection site, highly efficient gene expression could be observed in the knee joint transfected with the combination of BL and US exposure, compared to treatment with pDNA alone, pDNA plus BL, or pDNA plus US. The localized and prolonged gene expression was also shown by an in vivo luciferase imaging system. Thus, this local gene delivery system into joint synovium using the combination of BL and US exposure may be an effective means for gene therapy in joint disorders.

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Simultaneous regeneration of articular cartilage and subchondral bone in vivo using MSCs induced by a spatially controlled gene delivery system in bilayered integrated scaffolds

Biomaterials ◽

10.1016/j.biomaterials.2011.03.041 ◽

2011 ◽

Vol 32 (21) ◽

pp. 4793-4805 ◽

Cited By ~ 185

Author(s):

Jiangning Chen ◽

Huan Chen ◽

Pei Li ◽

Huajia Diao ◽

Shiyu Zhu ◽

...

Keyword(s):

Articular Cartilage ◽

Gene Delivery ◽

Delivery System ◽

Subchondral Bone ◽

Gene Delivery System

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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

Materials Science Forum ◽

10.4028/www.scientific.net/msf.539-543.641 ◽

2007 ◽

Vol 539-543 ◽

pp. 641-646 ◽

Cited By ~ 1

Author(s):

Tae Hee Kim ◽

Jin Hua ◽

Hyun Woo Kim ◽

Myung Haing Cho ◽

Jae Woon Nah ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Viral Gene ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Cell Specificity ◽

Selective Delivery

The development of an efficient targeted gene delivery system into cells is an important strategy for the advancement of gene therapy. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relative low transfection efficiency. And it also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency.

Download Full-text