1134: Gene Expression Profile Changes using CDNA Microarray Technology in Patients with Neurogenic Bladder Secondary to Spinal Cord Injury

AbstractSpinal cord injury (SCI) is often accompanied by muscle atrophy; however, its underlying mechanisms remain unclear. Here, the molecular mechanisms of muscle atrophy following SCI were investigated. The GSE45550 gene expression profile of control (before SCI) and experimental (14 days following SCI) groups, consisting of Sprague–Dawley rat soleus muscle (n = 6 per group), was downloaded from the Gene Expression Omnibus database, and then differentially expressed gene (DEG) identification and Gene Ontology, pathway, pathway network, and gene signal network analyses were performed. A total of 925 differentially expressed genes, 149 biological processes, and 55 pathways were screened. In the pathway network analysis, the 10 most important pathways were citrate cycle (TCA cycle), pyruvate metabolism, MAPK signalling pathway, fatty acid degradation, propanoate metabolism, apoptosis, focal adhesion, synthesis and degradation of ketone bodies, Wnt signalling, and cancer pathways. In the gene signal network analysis, the 10 most important genes were Acat1, Acadvl, Acaa2, Hadhb, Acss1, Oxct1, Hadha, Hadh, Acaca, and Cpt1b. Thus, we screened the key genes and pathways that may be involved in muscle atrophy after SCI and provided support for finding valuable markers for this disease.

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Identifying gene expression profile of spinal cord injury in rat by bioinformatics strategy

Molecular Biology Reports ◽

10.1007/s11033-014-3176-8 ◽

2014 ◽

Vol 41 (5) ◽

pp. 3169-3177 ◽

Cited By ~ 12

Author(s):

Lingjing Jin ◽

Zhourui Wu ◽

Wei Xu ◽

Xiao Hu ◽

Jin Zhang ◽

...

Keyword(s):

Gene Expression ◽

Spinal Cord ◽

Spinal Cord Injury ◽

Gene Expression Profile ◽

Expression Profile ◽

Cord Injury

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Gene expression profile of zebrafish exposed to hypoxia during development

Physiological Genomics ◽

10.1152/physiolgenomics.00128.2002 ◽

2003 ◽

Vol 13 (2) ◽

pp. 97-106 ◽

Cited By ~ 185

Author(s):

Christopher Ton ◽

Dimitri Stamatiou ◽

Choong-Chin Liew

Keyword(s):

Gene Expression ◽

Gene Expression Profile ◽

Cdna Microarray ◽

Expression Profile ◽

Clinical Implications ◽

Zebrafish Embryos ◽

Hypoxic Stress ◽

Microarray Technology ◽

Adaptive Responses ◽

Low Oxygen

Understanding how vertebrates respond to hypoxia can have important clinical implications. Fish have evolved the ability to survive long exposure to low oxygen levels. However, little is known about the specific changes in gene expression that result from hypoxia. In this study we used a zebrafish cDNA microarray to examine the expression of >4,500 genes in zebrafish embryos exposed to 24 h of hypoxia during development. We tested the hypotheses that hypoxia changes gene expression profile of the zebrafish embryos and that these changes can be reverted by reexposure to a normoxic (20.8% O2) environment. Our data were consistent with both of these hypotheses: indicating that zebrafish embryos undergo adaptive changes in gene expression in response to hypoxia. Our study provides a striking genetic portrait of the zebrafish embryos’ adaptive responses to hypoxic stress and demonstrates the utility of the microarray technology as a tool for analyzing complex developmental processes in the zebrafish.

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