1. The combined use of chromatographic separation of urinary 17-ketosteroid extracts on alumina columns and infra-red analysis of the fractions has permitted the identification of the major components in 5 mg. of a mixture of α ketosteroids.
2. In 4 non-rheumatic children, 4 rheumatic (inactive) children and 1 patient with active rheumatic carditis, the steroids identified in the urinary extracts were androsterone, Δ androstenolone, etiocholanolone and 11-keto etiocholanolone. Other steroids were present in insufficient quantity to be identified.
3. In 1 patient with active rheumatic carditis, the steroids excreted during an 8-day course of ACTH therapy were Δ androstenolone, etiocholanolone,11-hydroxy androsterone, 3 α, 11 β androstanediol-17-one, and 1 compound of possible clinical significance which has been reported by other investigators, the structural formula for which is not known.
4. There appears to be no inherent difference between the urinary ketosteroids of rheumatic (inactive) and non-rheumatic children.