Chloroform and carbon tetrachloride induce intrachromosomal recombination and oxidative free radicals in Saccharomyces cerevisiae

Author(s):  
Richard J Brennan ◽  
Robert H Schiestl
1990 ◽  
Vol 222 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Robert H. Schiestl ◽  
R. Daniel Gietz ◽  
P. J. Hastings ◽  
Ulrike Wintersberger

The photoinitiation of vinyl polymerization, sensitized by rhenium carbonyl in the presence of carbon tetrachloride, has been studied at A = 3650 Å. Although in some ways the course of reaction is similar to that observed with manganese carbonyl as photosensitizer (Bamford, Crowe & Wayne 1965 a ), yet one important new feature is found with rhenium carbonyl. After irradiation a relatively high ‘dark’ rate of polymerization persists for several hours, and leads ultimately to high conversions. Studies made to elucidate the mechanism of the dark reaction are presented in this paper. It is shown that the kinetic results are consistent with the formation of two photolytic fragments, only one of which reacts rapidly with carbon tetrachloride to generate free radicals; the other reacts more slowly, so that its concentration builds up gradually during irradiation and decays over several hours after the light has been turned off. The quantum yield for initiation in the light is only slightly greater than unity, the result expected if the second fragment reacts but slowly with carbon tetrachloride. A comparison of the initiation photosensitized by manganese and rhenium carbonyls makes possible certain generalizations about the photochemistry of the group VII metal carbonyls, and suggestions are made about the primary and secondary photolytic processes in these systems.


1990 ◽  
Vol 10 (6) ◽  
pp. 2485-2491 ◽  
Author(s):  
R H Schiestl ◽  
S Prakash

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.


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