RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

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Analysis of Meiotic Recombination Pathways in the Yeast Saccharomyces cerevisiae

Genetics ◽

10.1093/genetics/144.1.71 ◽

1996 ◽

Vol 144 (1) ◽

pp. 71-86 ◽

Cited By ~ 5

Author(s):

Yang Mao-Draayer ◽

Anne M Galbraith ◽

Douglas L Pittman ◽

Marc Cool ◽

Robert E Malone

Keyword(s):

Saccharomyces Cerevisiae ◽

Meiotic Recombination ◽

The Other ◽

Yeast Saccharomyces Cerevisiae ◽

Recombination Hotspot ◽

Early Genes ◽

Intrachromosomal Recombination ◽

Mutant Phenotypes ◽

Recombination Pathway

Abstract In the yeast, Saccharomyces cerevisiae, several genes appear to act early in meiotic recombination. HOPl and REDl have been classified as such early genes. The data in this paper demonstrate that neither a redl nor a hopl mutation can rescue the inviable spores produced by a rad52 spol3 strain; this phenotype helps to distinguish these two genes from other early meiotic recombination genes such as SPOll, REC104, or A4EI4. In contrast, either a redl or a hopl mutation can rescue a rad50S spol3 strain; this phenotype is similar to that conferred by mutations in the other early recombination genes (e.g., REC104). These two different results can be explained because the data presented here indicate that a rad50S mutation does not diminish meiotic intrachromosomal recombination, similar to the mutant phenotypes conferred by redl or hopl. Of course, REDl and HOPl do act in the normal meiotic interchromosomal recombination pathway; they reduce interchromosomal recombination to ~10% of normal levels. We demonstrate that a mutation in a gene (REC104) required for initiation of exchange is completely epistatic to a mutation in REDl. Finally, mutations in either HOPl or RED1 reduce the number of doublestrand breaks observed at the HIS2 meiotic recombination hotspot.

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Differential mismatch repair can explain the disproportionalities between physical distances and recombination frequencies of cyc1 mutations in yeast.

Genetics ◽

10.1093/genetics/119.1.21 ◽

1988 ◽

Vol 119 (1) ◽

pp. 21-34

Author(s):

C W Moore ◽

D M Hampsey ◽

J F Ernst ◽

F Sherman

Keyword(s):

Saccharomyces Cerevisiae ◽

Mismatch Repair ◽

Meiotic Recombination ◽

Mitotic Recombination ◽

The Other ◽

Recombination Rates ◽

Base Pairs ◽

Yeast Saccharomyces Cerevisiae ◽

X Ray ◽

Mismatched Base Pair

Abstract Recombination rates have been examined in two-point crosses of various defined cyc1 mutations that cause the loss or nonfunction of iso-1-cytochrome c in the yeast Saccharomyces cerevisiae. Recombinants arising by three different means were investigated, including X-ray induced mitotic recombination, spontaneous mitotic recombination, and meiotic recombination. Heteroallelic diploid strains were derived by crossing cyc1 mutants containing a series of alterations at or near the same site to cyc1 mutants containing alterations at various distances. Marked disproportionalities between physical distances and recombination frequencies were observed with certain cyc1 mutations, indicating that certain mismatched bases can significantly affect recombination. The marker effects were more pronounced when the two mutational sites of the heteroalleles were within about 20 base pairs, but separated by at least 4 base pairs. Two alleles, cyc1-163 and cyc1-166, which arose by G.C----C.G transversions at nucleotide positions 3 and 194, respectively, gave rise to especially high rates of recombination. Other mutations having different substitutions at the same nucleotide positions were not associated with abnormally high recombination frequencies. We suggest that these marker effects are due to the lack of repair of either G/G or C/C mismatched base pairs, while the other mismatched base pair of the heteroallele undergoes substantial repair. Furthermore, we suggest that diminished recombination frequencies are due to the concomitant repair of both mismatches within the same DNA tract.

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Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae

Molecular and Cellular Biology ◽

10.1128/mcb.12.4.1805-1814.1992 ◽

1992 ◽

Vol 12 (4) ◽

pp. 1805-1814

Author(s):

P Detloff ◽

T D Petes

Keyword(s):

Saccharomyces Cerevisiae ◽

Meiotic Recombination ◽

High Frequency ◽

Excision Repair ◽

Yeast Saccharomyces Cerevisiae ◽

Heteroduplex Dna ◽

Homologous Chromosomes ◽

His4 Gene

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.

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RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.

Molecular and Cellular Biology ◽

10.1128/mcb.8.9.3619 ◽

1988 ◽

Vol 8 (9) ◽

pp. 3619-3626 ◽

Cited By ~ 128

Author(s):

R H Schiestl ◽

S Prakash

Keyword(s):

Saccharomyces Cerevisiae ◽

Genetic Recombination ◽

Excision Repair ◽

Point Mutations ◽

Gene Mutations ◽

The Other ◽

Linear Plasmids ◽

Repair Gene ◽

Mutant Strains ◽

Repair Genes

The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strains was examined. The rad1 deletion mutation and some rad1 point mutations reduced the frequency of intrachromosomal recombination of a his3 duplication, in which one his3 allele is deleted at the 3' end while the other his3 allele is deleted at the 5' end. Mutations in the other excision repair genes, RAD2, RAD3, and RAD4, did not lower recombination frequencies in the his3 duplication. As expected, recombination between the his3 deletion alleles in the duplication was reduced in the rad52 mutant. The frequency of HIS3+ recombinants fell synergistically in the rad1 rad52 double mutant, indicating that the RAD1 and RAD52 genes affect this recombination via different pathways. In contrast to the effect of mutations in the RAD52 gene, mutations in the RAD1 gene did not lower intrachromosomal and interchromosomal recombination between heteroalleles that carry point mutations rather than partial deletions; however, the rad1 delta mutation did lower the frequency of integration of linear plasmids and DNA fragments into homologous genomic sequences. We suggest that RAD1 plays a role in recombination after the formation of the recombinogenic substrate.

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Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae.

Molecular and Cellular Biology ◽

10.1128/mcb.12.4.1805 ◽

1992 ◽

Vol 12 (4) ◽

pp. 1805-1814 ◽

Cited By ~ 22

Author(s):

P Detloff ◽

T D Petes

Keyword(s):

Saccharomyces Cerevisiae ◽

Meiotic Recombination ◽

High Frequency ◽

Excision Repair ◽

Yeast Saccharomyces Cerevisiae ◽

Heteroduplex Dna ◽

Homologous Chromosomes ◽

His4 Gene

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.

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Interaction of excision repair gene products and mitotic recombination functions in yeast

Current Genetics ◽

10.1007/bf00351709 ◽

1993 ◽

Vol 24 (6) ◽

pp. 481-486 ◽

Cited By ~ 4

Author(s):

Beth A. Montelone ◽

Bee Choo Liang-Chong

Keyword(s):

Excision Repair ◽

Mitotic Recombination ◽

Gene Products ◽

Repair Gene

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RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination

Molecular and Cellular Biology ◽

10.1128/mcb.8.9.3619-3626.1988 ◽

1988 ◽

Vol 8 (9) ◽

pp. 3619-3626 ◽

Cited By ~ 1

Author(s):

R H Schiestl ◽

S Prakash

Keyword(s):

Saccharomyces Cerevisiae ◽

Genetic Recombination ◽

Excision Repair ◽

Point Mutations ◽

Gene Mutations ◽

The Other ◽

Linear Plasmids ◽

Repair Gene ◽

Mutant Strains ◽

Repair Genes

The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strains was examined. The rad1 deletion mutation and some rad1 point mutations reduced the frequency of intrachromosomal recombination of a his3 duplication, in which one his3 allele is deleted at the 3' end while the other his3 allele is deleted at the 5' end. Mutations in the other excision repair genes, RAD2, RAD3, and RAD4, did not lower recombination frequencies in the his3 duplication. As expected, recombination between the his3 deletion alleles in the duplication was reduced in the rad52 mutant. The frequency of HIS3+ recombinants fell synergistically in the rad1 rad52 double mutant, indicating that the RAD1 and RAD52 genes affect this recombination via different pathways. In contrast to the effect of mutations in the RAD52 gene, mutations in the RAD1 gene did not lower intrachromosomal and interchromosomal recombination between heteroalleles that carry point mutations rather than partial deletions; however, the rad1 delta mutation did lower the frequency of integration of linear plasmids and DNA fragments into homologous genomic sequences. We suggest that RAD1 plays a role in recombination after the formation of the recombinogenic substrate.

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XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination.

Genetics ◽

10.1093/genetics/132.3.651 ◽

1992 ◽

Vol 132 (3) ◽

pp. 651-664 ◽

Cited By ~ 6

Author(s):

E L Ivanov ◽

V G Korolev ◽

F Fabre

Keyword(s):

Saccharomyces Cerevisiae ◽

Dna Repair ◽

Gene Conversion ◽

Meiotic Recombination ◽

Repair Gene ◽

Strand Breaks ◽

Dna Repair Gene ◽

Mating Type Switching ◽

Radiation Induced ◽

Recombination Pathway

Abstract The XRS2 gene of Saccharomyces cerevisiae has been previously identified as a DNA repair gene. In this communication, we show that XRS2 also encodes an essential meiotic function. Spore inviability of xrs2 strains is rescued by a spo13 mutation, but meiotic recombination (both gene conversion and crossing over) is highly depressed in spo13 xrs2 diploids. The xrs2 mutation suppresses spore inviability of a spo13 rad52 strain suggesting that XRS2 acts prior to RAD52 in the meiotic recombination pathway. In agreement with the genetic data, meiosis-specific double-strand breaks at the ARG4 meiotic recombination hotspot are not detected in xrs2 strains. Despite its effects on meiotic recombination, the xrs2 mutation does not prevent mitotic recombination events, including homologous integration of linear DNA, mating-type switching and radiation-induced gene conversion. Moreover, xrs2 strains display a mitotic hyper-rec phenotype. Haploid xrs2 cells fail to carry out G2-repair of gamma-induced lesions, whereas xrs2 diploids are able to perform some diploid-specific repair of these lesions. Meiotic and mitotic phenotypes of xrs2 cells are very similar to those of rad50 cells suggesting that XRS2 is involved in homologous recombination in a way analogous to that of RAD50.

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CHROMOSOMAL TRANSLOCATIONS GENERATED BY HIGH-FREQUENCY MEIOTIC RECOMBINATION BETWEEN REPEATED YEAST GENES

Genetics ◽

10.1093/genetics/114.3.731 ◽

1986 ◽

Vol 114 (3) ◽

pp. 731-752

Author(s):

Sue Jinks-Robertson ◽

Thomas D Petes

Keyword(s):

Saccharomyces Cerevisiae ◽

Gene Conversion ◽

Meiotic Recombination ◽

High Frequency ◽

Mitotic Recombination ◽

Chromosomal Translocations ◽

Yeast Saccharomyces Cerevisiae ◽

Homologous Chromosomes ◽

Yeast Genes ◽

Repeated Genes

ABSTRACT We have examined meiotic and mitotic recombination between repeated genes on nonhomologous chromosomes in the yeast Saccharomyces cerevisiae . The results of these experiments can be summarized in three statements. First, gene conversion events between repeats on nonhomologous chromosomes occur frequently in meiosis. The frequency of such conversion events is only 17-fold less than the analogous frequency of conversion between genes at allelic positions on homologous chromosomes. Second, meiotic and mitotic conversion events between repeated genes on nonhomologous chromosomes are associated with reciprocal recombination to the same extent as conversion between allelic sequences. The reciprocal exchanges between the repeated genes result in chromosomal translocations. Finally, recombination between repeated genes on nonhomologous chromosomes occurs much more frequently in meiosis than in mitosis.

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