The aromatic amine carcinogens o-toluidine and o-anisidine induce free radicals and intrachromosomal recombination in Saccharomyces cerevisiae

This study investigated the effects of the electrolytic treatment in the simulated wastewater with aromatic amine n-phenyl-n-isopropyl-p-phenylenediamine (Flexzone 3P®) using Ti/TiRuO2 electrodes under 0.025 A/cm² (DC) for different electrolysis durations (5; 15; 30; 45 and 60 min). Conductivity, pH, UV-visible spectra, gas chromatograms, toxicity and biodegradation tests were carried out. During the electrolytic treatment the pH decreased and conductivity increased slightly. After 60 min of electrolysis, the concentration of Flexzone 3P decreased by 65.1%. UV-vis spectra and chromatograms of simulated wastewater showed changes in the molecular structure of the aromatic amine. After 5 and 15 min of electrolysis, the simulated wastewater containing the Flexzone 3P showed detoxification by Saccharomyces cerevisiae toxicity test. The electrolysis of 5 min improved the biodegradation of the simulated wastewater containing Flexzone 3P.

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Effect of benzene and its closed ring metabolites on intrachromosomal recombination in Saccharomyces cerevisiae

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/j.mrfmmm.2005.06.026 ◽

2006 ◽

Vol 593 (1-2) ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Christopher H. Sommers ◽

Robert H. Schiestl

Keyword(s):

Saccharomyces Cerevisiae ◽

Intrachromosomal Recombination ◽

Closed Ring

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RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.

Molecular and Cellular Biology ◽

10.1128/mcb.10.6.2485 ◽

1990 ◽

Vol 10 (6) ◽

pp. 2485-2491 ◽

Cited By ~ 95

Author(s):

R H Schiestl ◽

S Prakash

Keyword(s):

Saccharomyces Cerevisiae ◽

Meiotic Recombination ◽

Excision Repair ◽

Mitotic Recombination ◽

The Other ◽

Gene Products ◽

Repair Gene ◽

Homologous Chromosomes ◽

Intrachromosomal Recombination ◽

Recombination Pathway

The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RAD10 gene is also required for mitotic recombination. The rad10 delta mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3 delta 3' his3 delta 5'). The rate of formation of HIS3+ recombinants in the rad10 delta mutant was not affected by the rad1 delta mutation but decreased synergistically in the presence of the rad10 delta mutation in combination with the rad52 delta mutation. These observations indicate that the RAD1 and RAD10 genes function together in a mitotic recombination pathway that is distinct from the RAD52 recombination pathway. The rad10 delta mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RAD1 and RAD10 gene products act in recombination after the formation of the recombinogenic substrate. The rad1 delta and rad10 delta mutations did not affect meiotic intrachromosomal recombination of the his3 delta 3' his3 delta 5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.

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