Rhizobium leguminosarum bv. viciae populations in soils with increasing heavy metal contamination: abundance, plasmid profiles, diversity and metal tolerance

2002 ◽  
Vol 34 (4) ◽  
pp. 519-529 ◽  
Author(s):  
Amir Lakzian ◽  
Phillip Murphy ◽  
Andrew Turner ◽  
Jim L. Beynon ◽  
Ken E. Giller
Keyword(s):  
Heavy Metal ◽  
Metal Contamination ◽  
Rhizobium Leguminosarum ◽  
Heavy Metal Contamination ◽  
Metal Tolerance ◽  
Plasmid Profiles

scholarly journals Ancient Heavy Metal Contamination in Soils as a Driver of Tolerant Anthyllis vulneraria Rhizobial Communities

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
Roba Mohamad ◽  
Geraldine Maynaud ◽  
Antoine Le Quéré ◽  
Céline Vidal ◽  
Agnieszka Klonowska ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Metal Tolerance ◽  
Taxonomic Composition ◽  
Soil Conditions ◽  
Site Specific ◽  
Content Type ◽  
Anthyllis Vulneraria ◽  
Metal Levels

ABSTRACT Anthyllis vulneraria is a legume associated with nitrogen-fixing rhizobia that together offer an adapted biological material for mine-soil phytostabilization by limiting metal pollution. To find rhizobia associated with Anthyllis at a given site, we evaluated the genetic and phenotypic properties of a collection of 137 rhizobia recovered from soils presenting contrasting metal levels. Zn-Pb mine soils largely contained metal-tolerant rhizobia belonging to Mesorhizobium metallidurans or to another sister metal-tolerant species. All of the metal-tolerant isolates harbored the cadA marker gene (encoding a metal-efflux PIB-type ATPase transporter). In contrast, metal-sensitive strains were taxonomically distinct from metal-tolerant populations and consisted of new Mesorhizobium genospecies. Based on the symbiotic nodA marker, the populations comprise two symbiovar assemblages (potentially related to Anthyllis or Lotus host preferences) according to soil geographic locations but independently of metal content. Multivariate analysis showed that soil Pb and Cd concentrations differentially impacted the rhizobial communities and that a rhizobial community found in one geographically distant site was highly divergent from the others. In conclusion, heavy metal levels in soils drive the taxonomic composition of Anthyllis-associated rhizobial populations according to their metal-tolerance phenotype but not their symbiotic nodA diversity. In addition to heavy metals, local soil physicochemical and topoclimatic conditions also impact the rhizobial beta diversity. Mesorhizobium communities were locally adapted and site specific, and their use is recommended for the success of phytostabilization strategies based on Mesorhizobium-legume vegetation. IMPORTANCE Phytostabilization of toxic mine spoils limits heavy metal dispersion and environmental pollution by establishing a sustainable plant cover. This eco-friendly method is facilitated by the use of selected and adapted cover crop legumes living in symbiosis with rhizobia that can stimulate plant growth naturally through biological nitrogen fixation. We studied microsymbiont partners of a metal-tolerant legume, Anthyllis vulneraria, which is tolerant to very highly metal-polluted soils in mining and nonmining sites. Site-specific rhizobial communities were linked to taxonomic composition and metal tolerance capacity. The rhizobial species Mesorhizobium metallidurans was dominant in all Zn-Pb mines but one. It was not detected in unpolluted sites where other distinct Mesorhizobium species occur. Given the different soil conditions at the respective mining sites, including their heavy-metal contamination, revegetation strategies based on rhizobia adapting to local conditions are more likely to succeed over the long term compared to strategies based on introducing less-well-adapted strains.

scholarly journals Evaluation of Heavy Metal Tolerance Level of the Antarctic Bacterial Community in Biodegradation of Waste Canola Oil

2021 ◽  
Vol 13 (19) ◽  
pp. 10749
Author(s):  
Khadijah Nabilah Mohd Zahri ◽  
Claudio Gomez-Fuentes ◽  
Suriana Sabri ◽  
Azham Zulkharnain ◽  
Khalilah Abdul Khalil ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Bacterial Community ◽  
Bacterial Growth ◽  
Canola Oil ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Degrading Bacteria

Heavy metal contamination is accidentally becoming prevalent in Antarctica, one of the world’s most pristine regions. Anthropogenic as well as natural causes can result in heavy metal contamination. Each heavy metal has a different toxic effect on various microorganisms and species, which can interfere with other pollutant bioremediation processes. This study focused on the effect of co-contaminant heavy metals on waste canola oil (WCO) biodegradation by the BS14 bacterial community collected from Antarctic soil. The toxicity of different heavy metals in 1 ppm of concentration to the WCO-degrading bacteria was evaluated and further analyzed using half maximal inhibition concentration (IC50) and effective concentration (EC50) tests. The results obtained indicated that Ag and Hg significantly impeded bacterial growth and degradation of WCO, while interestingly, Cr, As, and Pb had the opposite effect. Meanwhile, Cd, Al, Zn, Ni, Co, and Cu only slightly inhibited the bacterial community in WCO biodegradation. The IC50 values of Ag and Hg for WCO degradation were found to be 0.47 and 0.54 ppm, respectively. Meanwhile, Cr, As, and Pb were well-tolerated and induced bacterial growth and WCO degradation, resulting in the EC50 values of 3.00, 23.80, and 28.98 ppm, respectively. The ability of the BS14 community to tolerate heavy metals while biodegrading WCO in low-temperature conditions was successfully confirmed, which is a crucial aspect in biodegrading oil due to the co-contamination of oil and heavy metals that can occur simultaneously, and at the same time it can be applied in heavy metal-contaminated areas.

ASSESSMENT OF HEAVY METAL CONTAMINATION AND IMPACT ON SURFACE WATERS IN IASI CITY, ROMANIA

2009 ◽  
Vol 8 (6) ◽  
pp. 1541-1551
Author(s):  
Corneliu Horaicu ◽  
Florea Cornel Gabrian ◽  
Irina Grozavu ◽  
Catalin Constantin Calu ◽  
Monica Horaicu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Surface Waters ◽  
Metal Contamination ◽  
Heavy Metal Contamination

Esr Indication of Heavy Metal Contamination of Urban Atmosphere

1993 ◽  
Vol 27 (7-8) ◽  
pp. 263-269 ◽  
Author(s):  
B. Iosefzon-Kuyavskaya ◽  
N. Myrlyan ◽  
A. Shames
Keyword(s):  
Heavy Metal ◽  
Air Pollution ◽  
Electron Spin Resonance ◽  
Free Radical ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Urban Atmosphere ◽  
Stable Free Radical ◽  
Spin Resonance ◽  
Line Characteristic

Electron Spin Resonance (ESR) was used for the examination oi dust samples collected from snow in an urban area. On the main doublet ESR line attributed to the signal of paramagnetic metals, a singlet line characteristic for stable free radical centers (FRC) was observed. A negative correlation of significant level between FRC signal intensity and heavy metal (HM) content was established. It was shown that FRC line intensity of dust may be used as a surrogate parameter for the estimation of air pollution by HM.

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