Simultaneous determination of caffeine and non-steroidal anti-inflammatory drugs in pharmaceutical formulations and blood plasma by reversed-phase HPLC from linear gradient elution

An improved LC-MS/MS method was developed for simultaneous determination of eleven bioactive constituents of Radix Angelicae Pubescentis and its related preparations. It was the first report on the quantification of bioactive constituents in different preparations of Radix Angelicae Pubescentis by LC-MS/MS analytical method. These samples were separated with an Agilent Zorbax Extend reversed-phase C18 column (1.8 μm, 4.6 × 100 mm) by linear gradient elution using aqueous ammonium acetate and acetonitrile as mobile phase. The flow rate was 0.3 mL min−1. The eleven bioactive constituents showed good regression(R>0.990)within test ranges and the recoveries were in the range of 87.1–110%. The limit of detections and quantifications for most of the major constituents were less than 0.5 and 1.0 ng mL−1, respectively. All results indicated that the developed method could be readily utilized as a suitable quality control method for Radix Angelicae Pubescentis and related preparations.

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Simultaneous Determination of Ephedrine and 2-Imidazolines in Pharmaceutical Formulations by Reversed-Phase Hplc

Journal of Liquid Chromatography ◽

10.1080/10826079508010447 ◽

1995 ◽

Vol 18 (16) ◽

pp. 3233-3242 ◽

Cited By ~ 14

Author(s):

D. De Orsi ◽

L. Gagliardi ◽

G. Cavazzutti ◽

M. G. Mediati ◽

D. Tonelli

Keyword(s):

Simultaneous Determination ◽

Pharmaceutical Formulations ◽

Reversed Phase ◽

Reversed Phase Hplc

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Simultaneous Determination of Ibuprofen and Pseudoephedrine Hydrochloride in Pharmaceutical Tablets by Reversed-Phase HPLC

Analytical Letters ◽

10.1080/00032710903202006 ◽

2009 ◽

Vol 42 (18) ◽

pp. 2951-2961 ◽

Cited By ~ 4

Author(s):

Marie-Hélène Langlois ◽

Philippe Dallet ◽

Tina Kauss ◽

Jean-Pierre Dubost

Keyword(s):

Simultaneous Determination ◽

Reversed Phase ◽

Reversed Phase Hplc ◽

Pharmaceutical Tablets

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Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

E-Journal of Chemistry ◽

10.1155/2011/360431 ◽

2011 ◽

Vol 8 (1) ◽

pp. 340-346 ◽

Cited By ~ 5

Author(s):

Rajesh M. Kashid ◽

Santosh G. Singh ◽

Shrawan Singh

Keyword(s):

High Performance Liquid Chromatography ◽

Simultaneous Determination ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Hplc Method ◽

Reversed Phase Hplc ◽

Methyl Paraben ◽

Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

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