Morphogenetic Investigations on in vitro Leaf Culture of Tomato (Lycopersicon esculentum Mill. cv. Starfire) and high Frequency Plant Regeneration

High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 %) was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l), TDZ (0.1 to 1.2 mg/l) and coconut water (0.1 to 0.9 ml/l) induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg), number of hands in a bunch, number of fingers in a hand, length of the finger (cm), girth of the finger (cm) and girth of the pseudostem (cm) exhibited by in vitro plants were higher than the in vivo plants.Int J Appl Sci Biotechnol, Vol 3(2): 202-209 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12536

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In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

Canadian Journal of Botany ◽

10.1139/b76-254 ◽

1976 ◽

Vol 54 (21) ◽

pp. 2409-2414 ◽

Cited By ~ 34

Author(s):

R. M. Behki ◽

S. M. Lesley

Keyword(s):

Plant Regeneration ◽

Lycopersicon Esculentum ◽

Growth Regulators ◽

In Vitro Plant Regeneration ◽

Leaf Discs ◽

Skoog Medium ◽

Murashige And Skoog Medium ◽

Napthaleneacetic Acid ◽

In Vitro Plant

Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic acid (NAA) and benzylaminopurine (BA) and 4 combinations of NAA and zeatin. The results show that either callus, shoots, roots, or shoots and roots can be produced depending upon the hormone concentrations and ratios. Plants were regenerated from 12 of the 15 varieties tested.

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Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds

Journal of Plant Sciences ◽

10.3923/jps.2015.15.24 ◽

2014 ◽

Vol 10 (1) ◽

pp. 15-24 ◽

Cited By ~ 1

Author(s):

Chirom Oceania ◽

Thingujam Doni ◽

Leimapokpam Tikendra ◽

Potshangbam Nongdam

Keyword(s):

Lycopersicon Esculentum ◽

In Vitro Culture ◽

Synthetic Seeds ◽

Lycopersicon Esculentum Mill

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High frequency plant regeneration from leaf culture of Neolamarckia cadamba

Plant Biotechnology ◽

10.5511/plantbiotechnology.18.1119a ◽

2019 ◽

Vol 36 (1) ◽

pp. 13-19 ◽

Cited By ~ 3

Author(s):

Jingjian Li ◽

Deng Zhang ◽

Kunxi Ouyang ◽

Xiaoyang Chen

Keyword(s):

Plant Regeneration ◽

High Frequency ◽

Leaf Culture

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In vitro culture of young floral buds of tomato (Lycopersicon esculentum mill.)

Plant Science ◽

10.1016/0168-9452(86)90182-2 ◽

1986 ◽

Vol 47 (3) ◽

pp. 221-227 ◽

Cited By ~ 10

Author(s):

Rajeev Rastogi ◽

Vipen K. Sawhney

Keyword(s):

Lycopersicon Esculentum ◽

In Vitro Culture ◽

Lycopersicon Esculentum Mill ◽

Floral Buds

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In vitro Regeneration and Agrobacterium-mediated Genetic Transformation of Tomato (Lycopersicon esculentum Mill.)

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v19i1.5004 ◽

1970 ◽

Vol 19 (1) ◽

pp. 101-111 ◽

Cited By ~ 1

Author(s):

Rakha Hari Sarker ◽

Khaleda Islam ◽

M.I. Hoque

Keyword(s):

Lycopersicon Esculentum ◽

Genetic Transformation ◽

In Vitro Regeneration ◽

Leaf Explants ◽

Multiple Shoots ◽

Multiple Shoot ◽

Root Induction ◽

Cotyledonary Leaf ◽

Lycopersicon Esculentum Mill

Agrobacterium-mediated genetic transformation system has been developed for two tomato (Lycopersicon esculentum Mill.) varieties, namely Pusa Ruby (PR) and BARI Tomato-3 (BT-3). Prior to the establishment of transformation protocol cotyledonary leaf explants from the two varieties were cultured to obtain genotype independent in vitro regeneration. Healthy multiple shoot regeneration was obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength of MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants. Transformation ability of cotyledonary leaf explants was tested with Agrobacterium tumefaciens strain LBA4404 harboring binary plasmid pBI121, containing GUS and npt II genes. Transformed cotyledonary leaf explants were found to produce multiple shoots on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. Selection of the transformed shoots was carried out by gradually increasing the concentration of kanamycin to 200 mg/l since kanamycin resistant gene was used for transformation experiments. Shoots that survived under selection pressure were subjected to rooting. Transformed rooted plantlets were transferred to soil. Stable expression of GUS gene was detected in the various tissues from putatively transformed plantlets using GUS histochemical assay. Key words: In vitro regeneration, transformation, tomato D.O.I. 10.3329/ptcb.v19i1.5004 Plant Tissue Cult. & Biotech. 19(1): 101-111, 2009 (June)

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Avaliação da capacidade de regeneração in vitro em tomateiro industrial

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2000000800004 ◽

2000 ◽

Vol 35 (8) ◽

pp. 1523-1529

Author(s):

MIKLÓS FÁRI ◽

GERALDO MILANEZ DE RESENDE ◽

NATONIEL FRANKLIN DE MELO

Keyword(s):

Lycopersicon Esculentum ◽

Lycopersicon Esculentum Mill

Este trabalho teve por objetivo avaliar a capacidade de regeneração das cultivares de tomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composições de meio de cultura descritos na literatura e cinco variações de inoculação. Foi testada uma nova variação de inoculação, denominada cotilédone fendido. A maior freqüência de formação de gemas vegetativas foi 100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foram necessários três subcultivos dos explantes apresentando gemas. No caso de IPA-5, o número de brotos obtidos foi maior quando a indução de gemas foi realizada em meio contendo BAP (2,5 mg L-1) e AIA (0,2 mg L-1) seguido de três subcultivos, em meio como zeatina (0,5 mg L-1). Usando esse protocolo, a cultivar IPA-5 produziu uma média de 5,45 brotos alongados a partir do cotilédone fendido. Essa capacidade excedeu significativamente o cotilédone aparado, que produziu 4,4 brotos alongados por explante. No caso de IPA-6, a melhor combinação de meios e método de inoculação produziu 0,87 broto alongado por explante. Os brotos alongados foram enraizados e transferidos para casa de vegetação.

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