High frequency induction of somatic embryos in cultured leaf expiants of Coffea arabica L.

1977 ◽  
Vol 81 (5) ◽  
pp. 395-408 ◽  
Author(s):  
M.R. Söndahl ◽  
W.R. Sharp
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Coffea Arabica L ◽  
High Frequency Induction

scholarly journals High-frequency Direct Somatic Embryogenesis and Plantlet Regeneration from Leaves Derived from In Vitro-Germinated Seedlings of a Coffea arabica Hybrid Cultivar

HortScience ◽  
2016 ◽  
Vol 51 (9) ◽  
pp. 1148-1152 ◽  
Author(s):  
Jane Kahia ◽  
Margaret Kirika ◽  
Hudson Lubabali ◽  
Sinclair Mantell
Keyword(s):  
Tissue Culture ◽  
High Frequency ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Alternative Methods ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Embryogenic Cultures ◽  
Half Strength

Breeding work carried out during the period 1971–85 by the Coffee Research Institute, Ruiru, Kenya resulted in the release of a new improved hybrid Coffea arabica named Ruiru 11. The cultivar combines resistance to coffee berry disease (CBD) and leaf rust, with high yield and good cup quality attributes. The propagation by F1 hybrid seeds production, cuttings, and tip grafting do not produce enough planting materials. There was a need to explore alternative methods and tissue culture offers potential options. The objective of the study was to evaluate the effect of explant sources and cytokinins on induction and regeneration of somatic embryos. Eight different explants were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 10 µm benzylaminopurine (BAP). The effect of kinetin, N6-(2-isopentyl) adenine (2iP) evaluated at (0, 0.5, 5, or 25 µm) or thidiazuron (TDZ) (0, 0.5, 1.0, or 5 µm) added in separate experiments was also evaluated. The percentage of embryogenic cultures and the numbers of embryos per explant were determined after 3 months’ culture. The explant type had a significant effect (P > 0.05) on the induction of somatic embryos. Explants from in vitro-germinated seedlings produced the highest embryogenic cultures (90%) and the highest mean number of embryos (19.36) per explant. Cytokinins strongly enhanced induction and regeneration of somatic embryos. TDZ at 1 µm produced the highest embryogenic cultures (100%) and the highest mean number of embryos (24.2). The embryos were germinated on half-strength MS medium without any hormones. A high (98%) survival rate of the regenerated plantlets was recorded over all the treatments in the greenhouse. This is the first report on induction of high-frequency direct somatic embryos from coffee juvenile tissues. This is of great significance in tissue culture and indeed molecular biology manipulations because it allows regeneration of coffee from several explants.

scholarly journals Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

Phyton ◽  
2021 ◽  
Vol 90 (6) ◽  
pp. 1741-1748
Author(s):  
Eliana Arias-P閞ez ◽  
Carlos Alberto Lecona-Guzm醤 ◽  
Federico Antonio Guti閞rez-Miceli ◽  
Joaqu韓 Adolfo Montes-Molina ◽  
Nancy Ruiz-Lau
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
In Vitro Preservation ◽  
Coffea Arabica L

scholarly journals Morphological Variation of Somatic Embryos of Coffea arabica L. During Some Sub-Culture Periods

2017 ◽  
Vol 33 (2) ◽  
pp. 97
Author(s):  
Fitria Ardiyani ◽  
Sulistyani Pancaningtya
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Histological Analysis ◽  
Culture Media ◽  
Culture Period ◽  
Plant Cultivation ◽  
Morphological Measurement ◽  
Murashige Skoog ◽  
Coffea Arabica L

One of factors that affects the success of a plant cultivation using somatic embryogenesis method is the formation of somatic embryos from embryogenesis callus. This research aimed to study the effect of sub-culture period on quality and quantity of the somatic embryos of Coffea arabica. This research used explants of somatic embryos of Arabica coffee obtained from the leaves of 2K Andungsari clone. The embryos were taken during embryogenes is callus phase using Murashige-Skoog culture media added with B5 vitamin and auxin hormone (2,4dichlorophenoxy acetic acid) 0.5 mg/L and sitokinin (benzyl amino purin) 1 mg/L. Observation on somatic embryos obtained from the sub-culture period of 3, 6, 9, 12 and 15 weeks. The parameters observed in this study included quantity and quality of the somatic embryos during each sub-culture period. Observations on quantity of the somatic embryos were conducted based on number of embryos per cluster, while quality was measured from the percentage of normal embryos, histological analysis, and morphological measurement on weight and size of the normal embryos. The result showed that the best quantity of somatic embryos was obtained from the sub-culture period of nine weeks with 18.4 somatic embryos per cluster. The best quality embryos were also obtained in the sub-culture period of nine weeks with the percentage of normal embryos 71.4%. Histological analysis carried out on the somatic embryos obtained from sub-culture of three weeks period showed that the cells of the embryos were formed by living and solid cells which nucleus were clearly seen in the center of the cell, indicating that the embryos were formed by young tissues. Data of morphological parameters showed that normal embryos during the sub-culture period 3 to 15 weeks weighed around 0.23–0.78 mg and length of around 0.18–0.25 cm. The data can be used to predict number of explants and required time to produce certain number of normal embryos

Comparative ontogenesis of Coffea arabica L. somatic embryos reveals the efficiency of regeneration modulated by the explant source and the embryogenesis pathway

2021 ◽  
Author(s):  
Ilse Fernanda Ferrari ◽  
Giovanna Arcolini Marques ◽  
Welington Luis Sachetti Junior ◽  
Bárbara Bort Biazotti ◽  
Matheus Pena Passos ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Explant Source ◽  
Coffea Arabica L

scholarly journals Pengaruh 2,4-D terhadap Induksi Embrio Somatik Kopi Arabika

2017 ◽  
Vol 10 (2) ◽  
pp. 82 ◽  
Author(s):  
Imron Riyadi ◽  
NFN Tirtoboma
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Plant Material ◽  
Culture Medium ◽  
Standard Medium ◽  
Arabica Coffee ◽  
Murashige Skoog ◽  
Young Leaves ◽  
Coffea Arabica L ◽  
Direct Induction

<p><strong>Abstract</strong></p><p>Direct induction of somatic embryos in Arabica coffee (Coffea arabica L.) using plant groth regulators (PGR's) has been successful. The concentration and combination of different kinds of PGR's can influence the response and success in embryo induction. An experiment was conducted to determine the optimal concentration of 2.4-D in combination with kinetin for direct induction and proliferation of somatic embryos. The plant material used was Arabica coffee var. Kartika-l originating from The Indonesian Coffee and Cacao Research Institute, Jember. Explants were taken from young leaves of reddish-green in color. Somatic embryos were induced directly on a Murashige-Skoog (MS) standard medium containing 30 g/l sucrose and supplemented with 0, 1, 2, 4, and 8 mg/l 2.4-D in combination with 0.1 mg/l kinetin each. The cultures were incubated in the dark at temperature 26oC and RH +60% for 6 weeks with 10 replications. The results showed that somatic embryogenesis in Arabica coffee was best induced in a culture medium wiyh 2.4-D at 4 mg/l, combination with 0.1 mg/l kinetin. Induction of somatic embryos was achieved at 100% 4 weeks after culture. Three morphological stages of embryo development were identified: globular, early heart, and middle heart. The embryos were of three distinct colors such as, yellowish, yellowish-white, and white. The highest rate of proliferation of somatic embryos was achieved at 2 mg/l, 2.4-D in combination with 0.1 mg/l kinetin averaging 68.53 embryos per explant 6 weeks after subculture.</p>

scholarly journals 2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

2018 ◽  
Vol 48 (11) ◽  
Author(s):  
Ivanilda dos Santos Alves ◽  
Valéria Cristina Barbosa Carmazini ◽  
Cosme Damião dos Santos ◽  
Julieta Andrea Silva de Almeida
Keyword(s):  
Somatic Embryogenesis ◽  
Salt Concentration ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Coffea Arabica L

ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.

scholarly journals Formation of Somatic Embryos from Protoplasts of Coffea arabica L.

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 172-174 ◽  
Author(s):  
Mari Tahara ◽  
Takeshi Yasuda ◽  
Naotsugu Uchida ◽  
Tadashi Yamaguchi
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Culture System ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Skoog Medium ◽  
Intact Plants ◽  
Murashige And Skoog Medium ◽  
Coffea Arabica L

Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 μm BA. Somatic embryos developed into intact plants. Mannitol at 0.5 m was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 m mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).

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