Comparative ontogenesis of Coffea arabica L. somatic embryos reveals the efficiency of regeneration modulated by the explant source and the embryogenesis pathway

2021 ◽  
Author(s):  
Ilse Fernanda Ferrari ◽  
Giovanna Arcolini Marques ◽  
Welington Luis Sachetti Junior ◽  
Bárbara Bort Biazotti ◽  
Matheus Pena Passos ◽  
...  
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Explant Source ◽  
Coffea Arabica L

scholarly journals Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

Phyton ◽  
2021 ◽  
Vol 90 (6) ◽  
pp. 1741-1748
Author(s):  
Eliana Arias-P閞ez ◽  
Carlos Alberto Lecona-Guzm醤 ◽  
Federico Antonio Guti閞rez-Miceli ◽  
Joaqu韓 Adolfo Montes-Molina ◽  
Nancy Ruiz-Lau
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
In Vitro Preservation ◽  
Coffea Arabica L

scholarly journals Morphological Variation of Somatic Embryos of Coffea arabica L. During Some Sub-Culture Periods

2017 ◽  
Vol 33 (2) ◽  
pp. 97
Author(s):  
Fitria Ardiyani ◽  
Sulistyani Pancaningtya
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Histological Analysis ◽  
Culture Media ◽  
Culture Period ◽  
Plant Cultivation ◽  
Morphological Measurement ◽  
Murashige Skoog ◽  
Coffea Arabica L

One of factors that affects the success of a plant cultivation using somatic embryogenesis method is the formation of somatic embryos from embryogenesis callus. This research aimed to study the effect of sub-culture period on quality and quantity of the somatic embryos of Coffea arabica. This research used explants of somatic embryos of Arabica coffee obtained from the leaves of 2K Andungsari clone. The embryos were taken during embryogenes is callus phase using Murashige-Skoog culture media added with B5 vitamin and auxin hormone (2,4dichlorophenoxy acetic acid) 0.5 mg/L and sitokinin (benzyl amino purin) 1 mg/L. Observation on somatic embryos obtained from the sub-culture period of 3, 6, 9, 12 and 15 weeks. The parameters observed in this study included quantity and quality of the somatic embryos during each sub-culture period. Observations on quantity of the somatic embryos were conducted based on number of embryos per cluster, while quality was measured from the percentage of normal embryos, histological analysis, and morphological measurement on weight and size of the normal embryos. The result showed that the best quantity of somatic embryos was obtained from the sub-culture period of nine weeks with 18.4 somatic embryos per cluster. The best quality embryos were also obtained in the sub-culture period of nine weeks with the percentage of normal embryos 71.4%. Histological analysis carried out on the somatic embryos obtained from sub-culture of three weeks period showed that the cells of the embryos were formed by living and solid cells which nucleus were clearly seen in the center of the cell, indicating that the embryos were formed by young tissues. Data of morphological parameters showed that normal embryos during the sub-culture period 3 to 15 weeks weighed around 0.23–0.78 mg and length of around 0.18–0.25 cm. The data can be used to predict number of explants and required time to produce certain number of normal embryos

scholarly journals Pengaruh 2,4-D terhadap Induksi Embrio Somatik Kopi Arabika

2017 ◽  
Vol 10 (2) ◽  
pp. 82 ◽  
Author(s):  
Imron Riyadi ◽  
NFN Tirtoboma
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Plant Material ◽  
Culture Medium ◽  
Standard Medium ◽  
Arabica Coffee ◽  
Murashige Skoog ◽  
Young Leaves ◽  
Coffea Arabica L ◽  
Direct Induction

<p><strong>Abstract</strong></p><p>Direct induction of somatic embryos in Arabica coffee (Coffea arabica L.) using plant groth regulators (PGR's) has been successful. The concentration and combination of different kinds of PGR's can influence the response and success in embryo induction. An experiment was conducted to determine the optimal concentration of 2.4-D in combination with kinetin for direct induction and proliferation of somatic embryos. The plant material used was Arabica coffee var. Kartika-l originating from The Indonesian Coffee and Cacao Research Institute, Jember. Explants were taken from young leaves of reddish-green in color. Somatic embryos were induced directly on a Murashige-Skoog (MS) standard medium containing 30 g/l sucrose and supplemented with 0, 1, 2, 4, and 8 mg/l 2.4-D in combination with 0.1 mg/l kinetin each. The cultures were incubated in the dark at temperature 26oC and RH +60% for 6 weeks with 10 replications. The results showed that somatic embryogenesis in Arabica coffee was best induced in a culture medium wiyh 2.4-D at 4 mg/l, combination with 0.1 mg/l kinetin. Induction of somatic embryos was achieved at 100% 4 weeks after culture. Three morphological stages of embryo development were identified: globular, early heart, and middle heart. The embryos were of three distinct colors such as, yellowish, yellowish-white, and white. The highest rate of proliferation of somatic embryos was achieved at 2 mg/l, 2.4-D in combination with 0.1 mg/l kinetin averaging 68.53 embryos per explant 6 weeks after subculture.</p>

scholarly journals 2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

2018 ◽  
Vol 48 (11) ◽  
Author(s):  
Ivanilda dos Santos Alves ◽  
Valéria Cristina Barbosa Carmazini ◽  
Cosme Damião dos Santos ◽  
Julieta Andrea Silva de Almeida
Keyword(s):  
Somatic Embryogenesis ◽  
Salt Concentration ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Coffea Arabica L

ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.

scholarly journals Formation of Somatic Embryos from Protoplasts of Coffea arabica L.

HortScience ◽  
1994 ◽  
Vol 29 (3) ◽  
pp. 172-174 ◽  
Author(s):  
Mari Tahara ◽  
Takeshi Yasuda ◽  
Naotsugu Uchida ◽  
Tadashi Yamaguchi
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Culture System ◽  
Coffea Arabica ◽  
Leaf Explants ◽  
Skoog Medium ◽  
Intact Plants ◽  
Murashige And Skoog Medium ◽  
Coffea Arabica L

Somatic embryos were regenerated from protoplasts isolated from embryogenic callus on young leaf explants from mature coffee trees. Embryos were regenerated on modified Murashige and Skoog medium supplemented with 5 μm BA. Somatic embryos developed into intact plants. Mannitol at 0.5 m was adequate as an osmoticum for isolating protoplasts, but subsequent culture required 0.3 m mannitol. A culture system in which osmolality was decreased gradually accelerated formation of colonies and somatic embryogenesis. Chemical name used: N-(phenylmethyl)-1H-purine-6-amine (BA).

SISTEMA DE PRODUCCIÓN DE CAFÉ (Coffea arabica L.) EN LA COMUNIDAD DEL CERRO CUATE, ILIATENCO, GUERRERO

2018 ◽  
Vol 11 (10) ◽  
Author(s):  
A. Delgado-Alvarado
Keyword(s):  
Coffea Arabica ◽  
El Sistema ◽  
Coffea Arabica L

Objetivo: Analizar el proceso de producción y comercialización de café en la comunidad del Cerro Cuate, Iliatenco, en la región de la Montaña de Guerrero, México.Diseño/Metodología/aproximación: el trabajo se realizó por; 1) investigación documental), 2) selección del área de estudio, 3) Entrevista estructurada con preguntas abiertas a 22 cafeticultores, soportada con la técnica de encuesta seccional y la herramienta de cédulas de entrevistas, y 4) análisis de la información. El tamaño de muestra se definió por el método de muestreo por conveniencia, y la selección de las unidades de análisis por la técnica bola de nieve. Resultados: El sistema de producción de café que predominó fue el sistema rústico de montaña, intercalado con plátano y frutales. La máxima productividad de las plantaciones se alcanza de 5.5 a 7.5años de edad, la renovación de plantas la hacen a los 12 años. El rendimiento de café fue de 3.7 kg por planta por año. La producción del café la realizan principalmente hombres (77.3%), entre 56 y 70años de edad. La venta se realiza en la presentación de café capulín a granel a intermediarios, a la ARIC, a CAFECO, a la Unión de Ejidos y a la Organización Mixtrui.Limitaciones del estudio/implicaciones: Se da una propuesta de mejora para favorecer el proceso de producción, beneficio y comercialización del café.Hallazgos/conclusiones: Coffea arabica es el principal café que se cultiva, con las variedades Typica, Caturra, Mundo Novo, Garnica y Bourbón. Los factores que limitan su producción y calidad son faltade planeación en manejo del cultivo y no contar con asesoría técnica.

Deposição da calda de pulverização em diferentes volumes vegetativos de Coffea arabica L.

2017 ◽  
Vol 12 (1) ◽  
pp. 69 ◽  
Author(s):  
Felipe Santinato ◽  
Renato Adriane Alves Ruas ◽  
Carlos Diego Silva ◽  
Rouverson Pereira Da Silva ◽  
Victor Afonso Reis Gonçalves ◽  
...  
Keyword(s):  
Coffea Arabica ◽  
Para Determinar ◽  
Coffea Arabica L

A disposição dos ramos e sobreposição das folhas em plantas de café dificultam a penetração da calda pulverizada. Portanto, para determinar o volume de calda adequado, é importante verificar o estado de enfolhamento da lavoura antes da aplicação. Objetivou-se com este trabalho, avaliar a deposição de caldas de pulverização em lavouras de café aplicadas em diferentes volumes vegetativos. Os tratamentos foram dispostos seguindo esquema de parcelas subdivididas em cada volume vegetativo (5.000; 7.500; 10.000 e 17.500 m<sup>3</sup> ha<sup>-1</sup>). Sendo as parcelas cinco volumes de calda (150, 300, 450, 600 e 750 L ha<sup>-1</sup>) e as subparcelas três posições no dossel do cafeeiro (terço superior, médio e inferior) com quatro repetições. Nos quatro volumes vegetativos estudados, não ocorreu interação significativa (p&gt;0,05) entre o volume aplicado e os diferentes volumes vegetativos. Porém, os volumes vegetativos de 5.000,0 e 17.500,0 m<sup>3</sup> ha<sup>-1</sup> a deposição foi crescente com aumento das vazões, o que pode ser atribuído a densidade foliar. Não foi verificada diferença estatística (p&gt; 0,05), entre as médias de deposição nos diferentes terços (alturas) no dossel das plantas de café, nos volumes vegetativos 5.000,0 m<sup>3 </sup>ha<sup>-1</sup>, 10.000,0 m<sup>3 </sup>ha<sup>-1</sup> e 17.500,0 m<sup>3 </sup>ha<sup>-1</sup>. Contudo, no volume de 7.500,0 m<sup>3 </sup>ha<sup>-1</sup> houve maior deposição no terço mediano em relação ao terço inferior. A deposição é maior à medida que aumenta o volume de calda aplicado. Em plantas com menor densidade foliar, há incremento na deposição. A deposição é maior no terço mediano em relação ao terço inferior.

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