scholarly journals Morphological Variation of Somatic Embryos of Coffea arabica L. During Some Sub-Culture Periods

2017 ◽  
Vol 33 (2) ◽  
pp. 97
Author(s):  
Fitria Ardiyani ◽  
Sulistyani Pancaningtya
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Histological Analysis ◽  
Culture Media ◽  
Culture Period ◽  
Plant Cultivation ◽  
Morphological Measurement ◽  
Murashige Skoog ◽  
Coffea Arabica L

One of factors that affects the success of a plant cultivation using somatic embryogenesis method is the formation of somatic embryos from embryogenesis callus. This research aimed to study the effect of sub-culture period on quality and quantity of the somatic embryos of Coffea arabica. This research used explants of somatic embryos of Arabica coffee obtained from the leaves of 2K Andungsari clone. The embryos were taken during embryogenes is callus phase using Murashige-Skoog culture media added with B5 vitamin and auxin hormone (2,4dichlorophenoxy acetic acid) 0.5 mg/L and sitokinin (benzyl amino purin) 1 mg/L. Observation on somatic embryos obtained from the sub-culture period of 3, 6, 9, 12 and 15 weeks. The parameters observed in this study included quantity and quality of the somatic embryos during each sub-culture period. Observations on quantity of the somatic embryos were conducted based on number of embryos per cluster, while quality was measured from the percentage of normal embryos, histological analysis, and morphological measurement on weight and size of the normal embryos. The result showed that the best quantity of somatic embryos was obtained from the sub-culture period of nine weeks with 18.4 somatic embryos per cluster. The best quality embryos were also obtained in the sub-culture period of nine weeks with the percentage of normal embryos 71.4%. Histological analysis carried out on the somatic embryos obtained from sub-culture of three weeks period showed that the cells of the embryos were formed by living and solid cells which nucleus were clearly seen in the center of the cell, indicating that the embryos were formed by young tissues. Data of morphological parameters showed that normal embryos during the sub-culture period 3 to 15 weeks weighed around 0.23–0.78 mg and length of around 0.18–0.25 cm. The data can be used to predict number of explants and required time to produce certain number of normal embryos

scholarly journals Pengaruh 2,4-D terhadap Induksi Embrio Somatik Kopi Arabika

2017 ◽  
Vol 10 (2) ◽  
pp. 82 ◽  
Author(s):  
Imron Riyadi ◽  
NFN Tirtoboma
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Plant Material ◽  
Culture Medium ◽  
Standard Medium ◽  
Arabica Coffee ◽  
Murashige Skoog ◽  
Young Leaves ◽  
Coffea Arabica L ◽  
Direct Induction

<p><strong>Abstract</strong></p><p>Direct induction of somatic embryos in Arabica coffee (Coffea arabica L.) using plant groth regulators (PGR's) has been successful. The concentration and combination of different kinds of PGR's can influence the response and success in embryo induction. An experiment was conducted to determine the optimal concentration of 2.4-D in combination with kinetin for direct induction and proliferation of somatic embryos. The plant material used was Arabica coffee var. Kartika-l originating from The Indonesian Coffee and Cacao Research Institute, Jember. Explants were taken from young leaves of reddish-green in color. Somatic embryos were induced directly on a Murashige-Skoog (MS) standard medium containing 30 g/l sucrose and supplemented with 0, 1, 2, 4, and 8 mg/l 2.4-D in combination with 0.1 mg/l kinetin each. The cultures were incubated in the dark at temperature 26oC and RH +60% for 6 weeks with 10 replications. The results showed that somatic embryogenesis in Arabica coffee was best induced in a culture medium wiyh 2.4-D at 4 mg/l, combination with 0.1 mg/l kinetin. Induction of somatic embryos was achieved at 100% 4 weeks after culture. Three morphological stages of embryo development were identified: globular, early heart, and middle heart. The embryos were of three distinct colors such as, yellowish, yellowish-white, and white. The highest rate of proliferation of somatic embryos was achieved at 2 mg/l, 2.4-D in combination with 0.1 mg/l kinetin averaging 68.53 embryos per explant 6 weeks after subculture.</p>

Exploring the multivariate technique in the discrimination of Coffea arabica L. cultivars regarding the production and quality of grains under the effect of water management

Euphytica ◽  
2021 ◽  
Vol 217 (6) ◽  
Author(s):  
Daniel Soares Ferreira ◽  
Guilherme Bravin Canal ◽  
Moyses Nascimento ◽  
Ana Carolina Campana Nascimento ◽  
João Marcos Soares Ferreira ◽  
...  
Keyword(s):  
Water Management ◽  
Coffea Arabica ◽  
Multivariate Technique ◽  
Effect Of Water ◽  
Coffea Arabica L

scholarly journals Production Process and Quality Testing of Arabica Ground Coffee (Coffee arabica L) Solok Regency, West Sumatera

2020 ◽  
Vol 4 (1) ◽  
pp. 36-55
Author(s):  
Rince Alfia Fadri ◽  
Kesuma Sayuti ◽  
Novizar Nazir ◽  
Irfan Suliansyah
Keyword(s):  
Production Process ◽  
Coffea Arabica ◽  
Arabica Coffee ◽  
Post Harvest ◽  
Coffee Arabica ◽  
Quality Testing ◽  
Harvesting Process ◽  
Coffea Arabica L ◽  
Ground Coffee

One of the types of coffee cultivated in Solok Regency is Arabica Coffee (Coffea Arabica L). The quality of the coffee produced can be influenced by the proper harvesting and post-harvest process so as to produce ground coffee with good quality. This research is done by the method of observation, descriptive, and hedonic test. Ground coffee produced in Solok Regency has a semi-mechanical harvesting process while the post-harvest process begins with the release of the skin and meat of the fruit, subsequent drying, and release of the skin of horns, sorting and grading, roasted, milling and packaging. This research aims to study the processing and quality of Arabica Ground Coffee in Solok Regency.

Climate and natural quality of Coffea arabica L. drink

2020 ◽  
Vol 141 (1-2) ◽  
pp. 87-98 ◽  
Author(s):  
Glauco de Souza Rolim ◽  
Lucas Eduardo de Oliveira Aparecido ◽  
Paulo Sérgio de Souza ◽  
Rubens Augusto Camargo Lamparelli ◽  
Éder Ribeiro dos Santos
Keyword(s):  
Coffea Arabica ◽  
Coffea Arabica L

Fruit thinning and shade improve bean characteristics and beverage quality of coffee (Coffea arabica L.) under optimal conditions

2005 ◽  
Vol 86 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Philippe Vaast ◽  
Benoit Bertrand ◽  
Jean-Jacques Perriot ◽  
Bernard Guyot ◽  
Michel Génard
Keyword(s):  
Coffea Arabica ◽  
Optimal Conditions ◽  
Fruit Thinning ◽  
Coffea Arabica L

scholarly journals Evaluation of the Extracellular Proteome Profile During the Somatic Embryogenesis Process of Coffea spp.

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Héctor Gabriel Mukul-López ◽  
Clelia De-la-Peña ◽  
Rosa María Galaz-Ávalos ◽  
Víctor Manuel Loyola-Vargas
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Culture Media ◽  
Coffea Canephora ◽  
Suspension Cultures ◽  
Extracellular Proteome ◽  
Molecular Events ◽  
The Media ◽  
Proteome Profile

The somatic embryogenesis (SE) has been used as an important tool for the study of molecular events in plant cell differentiation. Some studies have revealed that suspensions of somatic embryos secrete a vast array of proteins that could be involved in the regulation of this process. Many of these molecules have been suggested to work as inductors and others as inhibitors of the process. In the present work, suspension cultures of both <em>Coffea canephora</em> and <em>Coffea arabica</em> were used to study the population of proteins secreted into the media. Two types of cultures were used; one for the propagation of suspension cultures (non-embryogenic) and another for the induction of SE (embryogenic). The evaluated days were 14 and 42 for non-embryogenic condition and 21, 42, and 98 for the embryogenic condition. An embryogenic system was established in the <em>C. arabica</em> species, obtaining 4,000 embryos per liter. We analyzed the proteins secreted into the culture media, both under non-embryogenic and SE induction conditions. In <em>C. canephora</em> medium, we found 173 proteins after 14 d of culture under non-embryogenic conditions. In <em>C. arabica</em> we found 523 after 14 d under non-embryogenic conditions. Under embryogenic conditions we found 379, 409 and 175 proteins after 21, 42 and 98 d, respectively. We also determined that some proteins are secreted exclusively under embryogenic conditions and others proteins under non-embryogenic conditions.

scholarly journals Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

Phyton ◽  
2021 ◽  
Vol 90 (6) ◽  
pp. 1741-1748
Author(s):  
Eliana Arias-P閞ez ◽  
Carlos Alberto Lecona-Guzm醤 ◽  
Federico Antonio Guti閞rez-Miceli ◽  
Joaqu韓 Adolfo Montes-Molina ◽  
Nancy Ruiz-Lau
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
In Vitro Preservation ◽  
Coffea Arabica L
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