Optimization of semi-automated microsatellite multiplex polymerase chain reaction systems for rainbow trout (Oncorhynchus mykiss)

Aquaculture ◽  
1999 ◽  
Vol 172 (3-4) ◽  
pp. 247-254 ◽  
Author(s):  
Anthony G Fishback ◽  
Roy G Danzmann ◽  
Takashi Sakamoto ◽  
Moira M Ferguson
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Reaction Systems ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain ◽  
Microsatellite Multiplex

Identification of Atlantic Salmon (Salmo salar) and Rainbow Trout (Oncorhynchus mykiss) by Using Polymerase Chain Reaction Amplification and Restriction Analysis of the Mitochondrial Cytochrome b Gene

1998 ◽  
Vol 61 (4) ◽  
pp. 482-486 ◽  
Author(s):  
ESTHER CARRERA ◽  
TERESA GARCÍA ◽  
ANA CÉSPEDES ◽  
ISABEL GONZÁLEZ ◽  
BERNABÉ SANZ ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Oncorhynchus Mykiss ◽  
Cytochrome B ◽  
Salmo Salar ◽  
Cytochrome B Gene ◽  
Chain Reaction ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Polymerase Chain

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the identification of fresh and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Digestion of the 359-bp PCR product with the endonucleases EcoRV and TaqI yielded specific banding patterns for salmon and trout. This genetic marker can be very useful for detecting fraudulent substitution of the cheaper smoked trout for the more expensive smoked salmon.

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