[14] Purification of EEA1 from bovine brain cytosol using Rab5 affinity chromatography and activity assays

2001 ◽  
pp. 120-132 ◽  
Author(s):  
Savvas Christoforidis ◽  
Marino Zerial
Keyword(s):  
Affinity Chromatography ◽  
Bovine Brain ◽  
Brain Cytosol

Enzymatic formation of nitrogen oxides from L-arginine in bovine brain cytosol

1989 ◽  
Vol 165 (1) ◽  
pp. 284-291 ◽  
Author(s):  
Harald H.H.W. Schmidt ◽  
Petra Wilke ◽  
Birgit Evers ◽  
Eycke Böhme
Keyword(s):  
Nitrogen Oxides ◽  
Bovine Brain ◽  
Enzymatic Formation ◽  
Brain Cytosol

scholarly journals Studies on rat parotid-cell actomyosin

1984 ◽  
Vol 220 (1) ◽  
pp. 291-299 ◽  
Author(s):  
T Kealey ◽  
P J Randle
Keyword(s):  
Affinity Chromatography ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Bovine Brain ◽  
Cell Protein ◽  
Collagenase Digestion ◽  
Heat Treated ◽  
Parotid Cells ◽  
Rat Parotid

Actomyosin was partially purified from rat parotid cells dispersed by collagenase digestion and found to possess different solubility characteristics from that from (undispersed) rat parotid tissue. This is attributed to the decrease in vascular contamination effected by the isolation of parotid cells, yielding a non-muscle actomyosin [Adelstein, Conti, Johnson, Pastan & Pollard (1972) Proc. Natl. Acad. Sci. U.S.A. 69, 3693-3697]. Myosin light-chain kinase was partially purified from dispersed rat parotid cells by calmodulin affinity chromatography and shown to be activated by Ca2+-calmodulin. The calmodulin content of dispersed rat parotid cells was shown to be 6.50 +/- 0.59 ng of calmodulin/micrograms of rat parotid-cell protein (mean +/- S.E.M.), as determined by the activation of purified bovine brain phosphodiesterase by heat-treated extracts of dispersed rat parotid cells.

scholarly journals Metal-chelate affinity chromatography of cytosol proteins from bovine brain

1989 ◽  
Vol 49 ◽  
pp. 276
Author(s):  
Satoshi Matsushima ◽  
Akitoshi Kato ◽  
Kaname Nakatani ◽  
Shiro Waga ◽  
Toshio Tanaka
Keyword(s):  
Affinity Chromatography ◽  
Metal Chelate ◽  
Bovine Brain ◽  
Metal Chelate Affinity Chromatography

Partial purification and characterization of GDP dissociation stimulator (GDS) for the rho proteins from bovine brain cytosol

1990 ◽  
Vol 169 (2) ◽  
pp. 652-659 ◽  
Author(s):  
Mitsuo Isomura ◽  
Kozo Kaibuchi ◽  
Takeshi Yamamoto ◽  
Shiro Kawamura ◽  
Masaya Katayama ◽  
...  
Keyword(s):  
Bovine Brain ◽  
Partial Purification ◽  
Rho Proteins ◽  
Purification And Characterization ◽  
Brain Cytosol

scholarly journals Inhibition of Phospholipase D by a Protein Factor from Bovine Brain Cytosol

1996 ◽  
Vol 271 (41) ◽  
pp. 25213-25219 ◽  
Author(s):  
Jae Ho Kim ◽  
Yoon Jung Suh ◽  
Taehoon G. Lee ◽  
Yong Kim ◽  
Sun Sik Bae ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Bovine Brain ◽  
Protein Factor ◽  
Brain Cytosol

Complete amino acid sequence of a basic 21-kDa protein from bovine brain cytosol

1987 ◽  
Vol 166 (2) ◽  
pp. 333-338 ◽  
Author(s):  
Francoise SCHOENTGEN ◽  
Florence SACCOCCIO ◽  
Jacqueline JOLLES ◽  
Ida BERNIER ◽  
Pierre JOLLES
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Bovine Brain ◽  
Complete Amino Acid Sequence ◽  
Brain Cytosol
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