A sensitive monoclonal antibody ELISA for quantitication of the major olive pollen allergen Ole e 1: Applications and comparison with alternative methods

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in Northern Italy using SAT. The screening SAT (sSAT), using a monoclonal antibody cocktail-based ELISA to identify indoor and outdoor allergens, was performed. In all positive samples, an anti-IgE monoclonal antibody ELISA test was performed to extend panel of allergens. Out of 117 selected dogs, 69 were included in the study; 71% were positive and 29% were negative to sSAT. Among the 49 positive sSAT, 53% were positive for both indoor and outdoor, 38.8% only for indoor, and 8.2% only for outdoor allergens. This is the first study on the frequency of allergens involved in CAD in Italy using SAT. IgE hypersensitivity in atopic dogs of Northern Italy is usually associated with indoor allergens, primarily house dust mites. Among the outdoor allergens, an important role was played by Rumex acetosa. Polysensitization also commonly occurs. Therefore, since the numerous factors affect the IgE positivity in CAD, specific panels for geographical areas should be considered and re-evaluated at time intervals.

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Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods

Journal of Immunological Methods ◽

10.1016/0022-1759(87)90445-5 ◽

1987 ◽

Vol 98 (1) ◽

pp. 123-127 ◽

Cited By ~ 34

Author(s):

A.R. Freedman ◽

G. Galfrè ◽

E. Gal ◽

H.J. Ellis ◽

P.J. Ciclitira

Keyword(s):

Monoclonal Antibody ◽

Gluten Free ◽

Antibody Elisa ◽

Wheat Gliadin

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Epitope analysis of Japanese cedar pollen allergen Cry j1 with the human monoclonal antibody 4701-1

Human Antibodies ◽

10.3233/hab-140273 ◽

2014 ◽

Vol 22 (3-4) ◽

pp. 73-76 ◽

Cited By ~ 2

Author(s):

Yasunori Naganawa ◽

Mio Takeda ◽

Michie Shimmoto ◽

Hiroshi Shinmoto

Keyword(s):

Monoclonal Antibody ◽

Human Monoclonal Antibody ◽

Japanese Cedar ◽

Pollen Allergen ◽

Epitope Analysis ◽

Japanese Cedar Pollen ◽

Cedar Pollen

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Expression of the major olive pollen allergen Ole e 10 in the yeast Pichia pastoris: Evidence of post-translational modifications

Protein Expression and Purification ◽

10.1016/j.pep.2005.04.012 ◽

2005 ◽

Vol 44 (2) ◽

pp. 147-154 ◽

Cited By ~ 7

Author(s):

Patricia Barral ◽

Eva Batanero ◽

Mayte Villalba ◽

Rosalía Rodríguez

Keyword(s):

Pichia Pastoris ◽

Pollen Allergen ◽

Post Translational Modifications ◽

Yeast Pichia Pastoris ◽

Olive Pollen

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Monoclonal antibody ELISA test indicates that large amounts of constitutive hsp-70 are present in salamanders, turtle and fish

Journal of Thermal Biology ◽

10.1016/0306-4565(94)90008-6 ◽

1994 ◽

Vol 19 (1) ◽

pp. 41-53 ◽

Cited By ~ 35

Author(s):

Zhongmo Yu ◽

Wayne E. Magee ◽

James R. Spotila

Keyword(s):

Monoclonal Antibody ◽

Elisa Test ◽

Hsp 70 ◽

Antibody Elisa

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The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER

Journal of Cell Science ◽

10.1242/jcs.112.15.2501 ◽

1999 ◽

Vol 112 (15) ◽

pp. 2501-2509 ◽

Cited By ~ 3

Author(s):

J. de Dios Alche ◽

A.J. Castro ◽

A. Olmedilla ◽

M.C. Fernandez ◽

R. Rodriguez ◽

...

Keyword(s):

Endoplasmic Reticulum ◽

In Situ Hybridization ◽

Membrane System ◽

Polymerase Chain Reaction Analysis ◽

Pollen Allergen ◽

Transcriptional Level ◽

Tetrad Stage ◽

Olive Pollen ◽

And Storage

The distribution of Ole e I (the major olive pollen allergen) and its transcripts was investigated in the anther from premeiotic stages until the dehiscent pollen stage. Crude protein extracts were analyzed by immunoblotting and probed with a monoclonal antibody to Ole e I. The protein, with three variants, was found to accumulate from the early microspore stage onwards. In addition to the previously reported localization of the protein, Ole e I has been immunolocalized for the first time within the pollen wall and in the tapetum. Reverse transcription-polymerase chain reaction analysis using specific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards. No expression was found in control tissues such as petals, roots or leaves. Light microscopy in situ hybridization on developing flower buds and dehiscent pollen confirmed the transcripts to be present in both the microspores and the sporophytic tissue (tapetum). Labeling was found primarily in the tapetum, reaching the highest concentration in the cytoplasm of the developing and mature pollen, once tapetum started to degenerate. In situ hybridization at the transmission electron microscope level showed the transcripts to accumulate on ribosomes of the rough endoplasmic reticulum. These studies, together with others carried out previously by us, indicated that both synthesis and storage of Ole e I take place in the endoplasmic reticulum, coincidentally with the conspicuous changes suffered by this membrane system during pollen development. This process is most likely controlled at the transcriptional level. The localization of the protein in the pollen ectexine bring new insights into the function of the allergen, which are discussed.

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