Monoclonal antibody ELISA test indicates that large amounts of constitutive hsp-70 are present in salamanders, turtle and fish

1994 ◽  
Vol 19 (1) ◽  
pp. 41-53 ◽  
Author(s):  
Zhongmo Yu ◽  
Wayne E. Magee ◽  
James R. Spotila
Keyword(s):  
Monoclonal Antibody ◽  
Elisa Test ◽  
Hsp 70 ◽  
Antibody Elisa

scholarly journals Detection of Serum Allergen-Specific IgE in Atopic Dogs Tested in Northern Italy: Preliminary Study

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 358
Author(s):  
Morena Di Tommaso ◽  
Alessia Luciani ◽  
Paolo Emidio Crisi ◽  
Marica Beschi ◽  
Paolo Rosi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Specific Ige ◽  
Elisa Test ◽  
Northern Italy ◽  
House Dust Mites ◽  
Indoor Allergens ◽  
Allergic Skin ◽  
Antibody Elisa ◽  
Ige Mediated ◽  
Indoor And Outdoor

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in Northern Italy using SAT. The screening SAT (sSAT), using a monoclonal antibody cocktail-based ELISA to identify indoor and outdoor allergens, was performed. In all positive samples, an anti-IgE monoclonal antibody ELISA test was performed to extend panel of allergens. Out of 117 selected dogs, 69 were included in the study; 71% were positive and 29% were negative to sSAT. Among the 49 positive sSAT, 53% were positive for both indoor and outdoor, 38.8% only for indoor, and 8.2% only for outdoor allergens. This is the first study on the frequency of allergens involved in CAD in Italy using SAT. IgE hypersensitivity in atopic dogs of Northern Italy is usually associated with indoor allergens, primarily house dust mites. Among the outdoor allergens, an important role was played by Rumex acetosa. Polysensitization also commonly occurs. Therefore, since the numerous factors affect the IgE positivity in CAD, specific panels for geographical areas should be considered and re-evaluated at time intervals.

scholarly journals Fimbrial-Specific Monoclonal Antibody-Based ELISA for European Potato Strains of Erwinia chrysanthemi and Comparison to PCR

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 443-448 ◽  
Author(s):  
U. Singh ◽  
C. M. Trevors ◽  
S. H. De Boer ◽  
J. D. Janse
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Pectate Lyase ◽  
Unknown Origin ◽  
Elisa Test ◽  
Erwinia Chrysanthemi ◽  
Polymerase Chain Reaction Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antibody Elisa ◽  
Chain Reaction Test

A murine hybridoma cell line, named 6A6, was developed to produce monoclonal antibodies for serological detection of European potato strains of Erwinia chrysanthemi. The monoclonal antibodies were of the immunoglobulin G2b type and were shown to react with a fimbrial antigen by immuno-gold electron microscopy, and with the fibrillin protein by Western blotting. In enzyme-linked immunosorbent assay (ELISA), the monoclonal antibody reacted with all but two strains of E. chrysanthemi isolated from potato. One non-reactive strain originated from Australia and therefore was likely a different biovar, and the other strain was of unknown origin. The monoclonal antibody also reacted with 20 out of 36 strains of E. chrysanthemi isolated from hosts other than potato. A triple-antibody ELISA test utilizing monoclonal antibody 6A6 successfully detected E. chrysanthemi in infected potato stems and tubers but sensitivity was limited to about 107 CFU/ml, compared to a sensitivity of 103 CFU/ml for a polymerase chain reaction test using published primers directed to the pectate lyase gene.

Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods

1987 ◽  
Vol 98 (1) ◽  
pp. 123-127 ◽  
Author(s):  
A.R. Freedman ◽  
G. Galfrè ◽  
E. Gal ◽  
H.J. Ellis ◽  
P.J. Ciclitira
Keyword(s):  
Monoclonal Antibody ◽  
Gluten Free ◽  
Antibody Elisa ◽  
Wheat Gliadin

A sensitive monoclonal antibody ELISA for quantitication of the major olive pollen allergen Ole e 1: Applications and comparison with alternative methods

2002 ◽  
Vol 109 (1) ◽  
pp. S329-S329
Author(s):  
Juan A Asturias ◽  
Carmen Arilla ◽  
Ignacio Ibarrola ◽  
Elena Eraso ◽  
Jaime Algorta ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Pollen Allergen ◽  
Alternative Methods ◽  
Antibody Elisa ◽  
Olive Pollen

Salmonella contamination in pigs at slaughter and on the farm: A field study using an antibody ELISA test and a PCR technique

2007 ◽  
Vol 115 (3) ◽  
pp. 259-267 ◽  
Author(s):  
Bernhard Nowak ◽  
Theda von Müffling ◽  
Sujate Chaunchom ◽  
Jörg Hartung
Keyword(s):  
Field Study ◽  
Elisa Test ◽  
Antibody Elisa

Development of a double monoclonal antibody sandwich ELISA test forVerticillium dahliaeKleb.

2005 ◽  
Vol 16 (4) ◽  
pp. 283-291 ◽  
Author(s):  
Fatima Yucel ◽  
Seher Benlioglu ◽  
Aynur Basalp ◽  
Selma Ozturk ◽  
Kemal Benlioglu
Keyword(s):  
Monoclonal Antibody ◽  
Sandwich Elisa ◽  
Elisa Test

scholarly journals Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein

2016 ◽  
Vol 19 (4) ◽  
pp. 877-883 ◽  
Author(s):  
K. Han ◽  
D. Zhao ◽  
Y. Liu ◽  
Q. Liu ◽  
X. Huang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Elisa Test ◽  
Antibody Detection ◽  
Economic Losses ◽  
Structural Protein ◽  
Neutralization Titer ◽  
Viral Attachment ◽  
E Protein ◽  
Duck Tembusu Virus ◽  
Tembusu Virus

Abstract Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.

Use of a Monoclonal Antibody againstBabesia bovisMerozoite Surface Antigen-2c for the Development of a Competitive ELISA Test

2004 ◽  
Vol 1026 (1) ◽  
pp. 165-170 ◽  
Author(s):  
MARIANA DOMINGUEZ ◽  
OSVALDO ZABAL ◽  
SILVINA WILKOWSKY ◽  
IGNACIO ECHAIDE ◽  
SUSANA TORIONI DE ECHAIDE ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Surface Antigen ◽  
Elisa Test ◽  
Competitive Elisa
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