SEMI-SOLID AGAR MEDIA FOR RAPID CULTURE OF TUBERCLE BACILLI

This transmission electron microscopy (TEM) procedure was designed to examine a gram positive spore-forming bacillus in colony on various solid agar media with minimal artifact. Cellular morphology and organization of colonies embedded in Poly/Bed 812 resin (P/B) were studied. It is a modification of procedures used for undecalcified rat bone and Stomatococcus mucilaginosus.Cultures were fixed and processed at room temperature (RT) under a fume hood. Solutions were added with a Pasteur pipet and removed by gentle vacuum aspiration. Other equipment used is shown in Figure 3. Cultures were fixed for 17-18 h in 10-20 ml of RT 2% phosphate buffered glutaraldehyde (422 mosm/KgH2O) within 5 m after removal from the incubator. After 3 (30 m) changes in 0.15 M phosphate buffer (PB = 209-213 mosm/KgH2O, pH 7.39-7.41), colony cut-outs (CCO) were made with a scalpel.

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Routes for salmonella contamination of poultry meat: epidemiological study from hatchery to slaughterhouse

Epidemiology and Infection ◽

10.1017/s0950268802007380 ◽

2002 ◽

Vol 129 (2) ◽

pp. 253-265 ◽

Cited By ~ 111

Author(s):

M. HEYNDRICKX ◽

D. VANDEKERCHOVE ◽

L. HERMAN ◽

I. ROLLIER ◽

K. GRIJSPEERDT ◽

...

Keyword(s):

Horizontal Transmission ◽

Poultry Meat ◽

Contamination Level ◽

Solid Agar ◽

Determining Factors ◽

Production Stages ◽

Animal Material ◽

Semi Solid ◽

Therapeutic Doses ◽

Broiler House

Data were collected on the prevalence of salmonella at different stages during the life cycle of 18 broiler flocks on different farms as well as during slaughter in different poultry slaughterhouses. For the isolation of salmonella, the highest sensitivity (93.9%) was obtained by enrichment in the semi-solid agar Diasalm. The ‘overshoe method’ utilizing several pairs of overshoes provided the highest sensitivity for determining the salmonella status of the broilers during rearing. A clear decrease of the relative importance of the first production stages was demonstrated for the salmonella contamination of the end product, whereas horizontal transmission of salmonella to broilers during rearing and to broiler carcasses in the slaughterhouse was shown to be the main determinative factor. Ten of the 18 flocks received a salmonella positive status with the highest shedding occurring during the first 2 weeks of rearing. The shedding of the animals was significantly negatively influenced by the use of subtherapeutic or therapeutic doses of antibiotics. The intake of portable material in the broiler house was identified as the most important risk factor for horizontal transmission. Significant associations were found between the contamination level of a flock and hygiene of the broiler house, feed and water in the broiler house and both animal and non-animal material sampled in the environment. No correlation was found between contamination during the rearing period and contamination found after slaughtering. The presence of faecal material in the transport crates and predominantly the identity of the slaughterhouse seemed to be the determining factors for carcass quality. Improved hygiene management during transport of broilers and in some slaughterhouses could significantly reduce the risk of salmonella contamination of poultry meat.

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Aeromonas hydrophila in Shellfish Growing Waters: Incidence and Media Evaluation

Journal of Food Protection ◽

10.4315/0362-028x-52.1.7 ◽

1989 ◽

Vol 52 (1) ◽

pp. 7-12 ◽

Cited By ~ 10

Author(s):

CARLOS ABEYTA ◽

STEPHEN D. WEAGANT ◽

CHARLES A. KAYSNER ◽

MARLEEN M. WEKELL ◽

ROBERT F. STOTT ◽

...

Keyword(s):

Aeromonas Hydrophila ◽

Environmental Samples ◽

Enterotoxin Production ◽

Beef Extract ◽

Solid Agar ◽

Agar Media

Levels of Aeromonas hydrophila determined for the shellfish growing area of Grays Harbor, Washington, ranged from 3 to 4600/100 g in oysters and from 3 to 2400/100 ml in water. Of isolates tested, 80% produced a hemolysin, a trait reported to correlate with enterotoxin production and pathogenicity. Two enrichment broths, Tryptic Soy Broth with ampicillin (TSBA) and Modified Rimler Shotts Broth (MRSB) were compared in combination with three solid agar media: Rimler Shotts (RS), Peptone Beef Extract Glycogen (PBG), and MacConkey's (MCA) agars. TSBA was far superior to MRSB in isolating this species from the environmental samples tested.

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HIGH HUMAN ERYTHROID BURST-FORMING ACTIVITY IN SERA FROM CHILDHOOD APLASTIC ANAEMIA IN SEMI-SOLID AGAR

British Journal of Haematology ◽

10.1111/j.1365-2141.1980.tb05970.x ◽

1980 ◽

Vol 46 (2) ◽

pp. 303-305 ◽

Cited By ~ 4

Author(s):

Hisato Kigasawa ◽

Hikokazu Nisiihika

Keyword(s):

Aplastic Anaemia ◽

Solid Agar ◽

Semi Solid

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Colony formation by Bact. lactis aerogenes on solid media containing antibacterial agents

Proceedings of the Royal Society of London Series A - Mathematical and Physical Sciences ◽

10.1098/rspa.1952.0232 ◽

1952 ◽

Vol 215 (1123) ◽

pp. 565-565

Keyword(s):

Inoculum Size ◽

Survival Times ◽

Fluctuation Test ◽

Solid Media ◽

Statistical Variation ◽

Toxic Concentration ◽

Solid Agar ◽

Agar Media ◽

Different Cultures ◽

The Given

A study has been made of the formation of colonies of Bact. lactis aerogenes on solid agar media containing antibacterial substances (brilliant green, 1-phenyl semicarbazide, phenyl mercuric nitrate, phenol, thymol and chloramphenicol) at such concentrations that a small fraction only of the inoculated cells develop. The pattern of behaviour varies from drug to drug and sometimes from culture to culture with a given drug. As the toxic concentration increases, colonies diminish in number, in size or in both. Anomalous dependence in some cases upon inoculum size, and the appearance in others of satellites to the main colonies, indicate the operation of co-operative effects probably depending upon diffusion of metabolites or antagonists. The statistical variation in the number of developing colonies is greater for different cultures than for samples of a given culture (as in the well-known fluctuation test for mutations), but the behaviour of a culture may depend upon the aeration, and upon the precise conditions of the test. The variances show no apparent relation to the ease of production of resistance to the given drug. Nor does the scatter of the survival times in liquid media containing phenol (no resistance developable) differ much from that in chloramphenicol (resistant forms readily produced). Consideration of the factors determining the successful formation of a colony on a drug plate suggests that the fluctuation test for the demonstration of mutations must be applied with great reserve.

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