THE PRODUCTION OF HIGH-TITRE ANTIBODY AGAINST FREE ANGIOTENSIN II

The seroepidemiology of rubella infection in Hong Kong Chinese was examined by haemagglutination inhibition of normal sera and a comparison made where feasible with Caucasians living in Hong Kong. Taking reciprocal titre of 20 as a baseline, the incidence of maternally acquired antibody was 84% for Chinese and 90% for Caucasians. In babies up to 2 months this incidence was maintained in Caucasians but declined to 54% in Chinese. High titre antibody was more frequently detected in Caucasians generally, including women of child-bearing age. The detection of rubella-specific IgM in the Caucasian babies was suggestive of recent maternal infection. However, fewer Caucasian (20%) than Chinese (36%) women of child-bearing age (15–40 years) appeared unprotected against rubella. When all age groups were considered, 88% of Caucasians and 53% of Chinese were seropositive. The occurrence of a rubella outbreak during the study did not give rise to a significant increase in the incidence of seropositivity in Chinese 19–25 years, the only age group able to be evaluated in this manner. The ethnic differences in seroepidemiology are considered in the light of known HLA-1 and HLA-8 antigen distributions in Caucasian and Mongoloid people and the apparently low incidence of congenitally acquired rubella in Chinese.

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The radioimmunoassay of angiotensin II

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1969.0058 ◽

1969 ◽

Vol 173 (1032) ◽

pp. 327-338 ◽

Cited By ~ 17

Keyword(s):

Angiotensin Ii ◽

Lymph Nodes ◽

Specific Antibody ◽

Plasma Levels ◽

Intravenous Infusion ◽

High Titre ◽

Micro Particles ◽

Plasma Angiotensin

Specific antibody against free angiotensin II has been raised in high titre by immunizing rabbits with angiotensin II amide (‘Hypertensin’, Ciba) adsorbed on to micro-particles of carbon. The material was injected either parenterally or directly into lymph nodes and spleen. A radioimmunoassay capable of detecting 30 pg of angiotensin II has been developed, and extended for use in the estimation of angiotensin in circulating plasma. The validity of this application of the assay has been established in experiments where plasma levels of this hormone were studied in relation to the intravenous infusion of Val 5 -angiotensin II amide in humans. Present results of the investigation of the circulating plasma angiotensin II levels in various physiological and pathological states are presented.

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Immunogenicity of Streptococcus pneumoniae 74 kDa Surface Protein in Rabbit Model

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v31i1.28461 ◽

2016 ◽

pp. 25-28

Author(s):

Zeenat Jahan ◽

Iztiba Mallik Deeba ◽

Shahina Akter ◽

Tasmina Rahman ◽

Ashikun Nabi ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Extraction Procedure ◽

Rabbit Model ◽

Surface Protein ◽

Immunoblot Analysis ◽

High Titre ◽

Control Group ◽

Nitrocellulose Membrane ◽

Immunoblot Assay ◽

High Titre Antibody

Immunogenicity of a pneumococcal 74 kDa surface protein in the rabbit model was investigated in this study. For this, Streptococcus pneumoniae serotype 7F was collected and water extraction procedure was applied for the preparation of surface materials, which was used for further isolation of the 74 kDa surface protein. Rabbits were immunized with the sonicated nitrocellulose membrane containing the 74 kDa surface protein and the control group of rabbits was injected with same concentration of sonicated nitrocellulose membrane only. The antibody response against the 74 kDa surface protein was evaluated by both ELISA and immunoblot assay. Sera collected from the 74 kDa surface protein immunized rabbits were examined and the high titre (1:1600) ELISA values (OD490) indicated the production of adequate amounts of anti-74 kDa antibodies in the rabbits. In the immunoblot analysis, a single antigenic band was obtained on the nitrocellulose membrane treated with sera obtained from rabbits immunized with the 74 kDa surface protein. The high titre antibody production in the rabbits indicates the immunogenicity of the 74 kDa surface protein in rabbit model.Bangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 25-28

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