Peptide synthesis using proteases dissolved in organic solvents

1997 ◽  
Vol 20 (8) ◽  
pp. 623-628 ◽  
Author(s):  
Maria V. Sergeeva ◽  
Vikram M. Paradkar ◽  
Jonathan S. Dordick
Keyword(s):  
Organic Solvents ◽  
Peptide Synthesis

Peptide synthesis catalyzed by lipases in anhydrous organic solvents

1987 ◽  
Vol 109 (12) ◽  
pp. 3802-3804 ◽  
Author(s):  
Alexey L. Margolin ◽  
Alexander M. Klibanov
Keyword(s):  
Organic Solvents ◽  
Peptide Synthesis

Synthese von immobilisierten Peptidfragmenten an Polystyrol-Polyoxyethylen zur Affinitätschromatographie /Synthesis of Immobilized Peptide Fragments on Polystyrene-Polyoxyethylene for Affinity Chromatography

1987 ◽  
Vol 42 (4) ◽  
pp. 455-460 ◽  
Author(s):  
Ernst Bayer ◽  
Heribert Hellstern ◽  
Heiner Eckstein
Keyword(s):  
Affinity Chromatography ◽  
Organic Solvents ◽  
Peptide Synthesis ◽  
Stationary Phases ◽  
Peptide Bond ◽  
Protecting Groups ◽  
Peptide Fragments ◽  
Solid Supports ◽  
Polystyrene Beads ◽  
Acidic Conditions

Abstract Polystyrene-polyoxyethylene craft copolymers have been used for step-wise peptide synthesis. After completion of synthesis the protecting groups are cleaved under acidic conditions, where the polymer-peptide bond is stable. These gels in comparison to polystyrene peptide gels, show better properties for applications in affinity chromatography as well as synthesis on solid supports, because the advantageous properties of polystyrene beads are combined with the excellent spacer behavior of polyoxyethylene chains (mobility, solvation by water and organic solvents). Peptide gels with polylysine sequences have been synthesized as highly selective stationary phases for the separation of the homologous oligo desoxyribonucleotides (dT)n with n = 1-3. The principal possibilities of these gels for affinity chromatography is demonstrated.

Use of organic solvents paired by a hydrogen bond for peptide synthesis mediated by proteases

1993 ◽  
pp. 173-175
Author(s):  
Norikazu Nishino ◽  
Yuichi Makinose ◽  
Hisakazu Mihara ◽  
Tsutomu Fujimoto
Keyword(s):  
Hydrogen Bond ◽  
Organic Solvents ◽  
Peptide Synthesis
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