Epitope mapping for the Borrelia burgdorferi outer surface protein B of T-cell-lines of patients with lyme arthritis: Differential recognition in acute and chronic patients — evidence for epitope spreading in Lyme Arthritis?

1997 ◽  
Vol 56 ◽  
pp. 317
Author(s):  
M. Estrella ◽  
T. Kamradt
Keyword(s):  
T Cell ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Epitope Mapping ◽  
Surface Protein ◽  
Lyme Arthritis ◽  
Epitope Spreading ◽  
Chronic Patients ◽  
Differential Recognition ◽  
T Cell Lines

scholarly journals The T helper cell response in Lyme arthritis: differential recognition of Borrelia burgdorferi outer surface protein A in patients with treatment-resistant or treatment-responsive Lyme arthritis.

1994 ◽  
Vol 180 (6) ◽  
pp. 2069-2078 ◽  
Author(s):  
B Lengl-Janssen ◽  
A F Strauss ◽  
A C Steere ◽  
T Kamradt
Keyword(s):  
T Cell ◽  
Borrelia Burgdorferi ◽  
Cell Lines ◽  
Outer Surface ◽  
Cell Response ◽  
T Cell Response ◽  
Surface Proteins ◽  
Lyme Arthritis ◽  
Treatment Resistant ◽  
T Cell Lines

The host response to Borrelia burgdorferi is likely to play a role in the pathogenesis of Lyme arthritis. Whereas most patients with Lyme arthritis can be cured with antibiotic therapy, approximately 10% of the patients have persistent arthritis for months or even several years after antibiotic treatment. In this study, we tested the hypothesis that the T cell response to one or more antigens of B. burgdorferi is different in patients with treatment-responsive or treatment-resistant Lyme arthritis. For this purpose, 313 B. burgdorferi-specific T cell lines were derived from the synovial fluid or peripheral blood of four patients with treatment-responsive Lyme arthritis and five patients with treatment-resistant arthritis. 87 T cell lines from treatment-responsive Lyme arthritis and 112 lines from the treatment-resistant group were examined for the recognition of five recombinant. B. burgdorferi proteins: outer surface proteins A (OspA), B, C, p39, and p93. In both groups of patients, the T cell lines frequently recognized OspB, and only occasionally recognized OspC, p39, and p93. In contrast, OspA was preferentially recognized by T cell lines from patients with treatment-resistant arthritis, but only rarely recognized by T cell lines from patients with treatment-responsive arthritis (odds ratio 28.4, 95% confidence interval 9.2-87.8, p < 0.005). These results are compatible with the hypothesis that the T cell response to B. burgdorferi OspA is involved in the pathogenesis of treatment-resistant Lyme arthritis.

scholarly journals Borrelia burgdorferi activates a T helper type 1-like T cell subset in Lyme arthritis.

1991 ◽  
Vol 174 (3) ◽  
pp. 593-601 ◽  
Author(s):  
H Yssel ◽  
M C Shanafelt ◽  
C Soderberg ◽  
P V Schneider ◽  
J Anzola ◽  
...  
Keyword(s):  
T Cell ◽  
Borrelia Burgdorferi ◽  
Cell Subset ◽  
Chronic Inflammatory Disease ◽  
T Cell Response ◽  
Lyme Arthritis ◽  
T Helper ◽  
Cell Clones ◽  
T Cell Lines

18 cloned T cell lines reactive with Borrelia burgdorferi proteins, all CD3+4+8-TCR-alpha/beta+ and restricted by HLA class II proteins, were isolated from four patients with chronic Lyme arthritis. Analysis of these T cell clones indicated that the T cell response to the Lyme disease spirochete is not oligoclonally restricted; yet all produced the same pattern of lymphokines, resembling that of murine type 1 T helper cells, after antigen-specific or nonspecific stimulation. Therefore, a subset of human CD4+ T cells, with a distinct profile of lymphokine secretion, is selectively activated by the pathogen inciting this chronic inflammatory disease.

scholarly journals Occurrence of Severe Destructive Lyme Arthritis in Hamsters Vaccinated with Outer Surface Protein A and Challenged with Borrelia burgdorferi

2000 ◽  
Vol 68 (2) ◽  
pp. 658-663 ◽  
Author(s):  
Cindy L. Croke ◽  
Erik L. Munson ◽  
Steven D. Lovrich ◽  
John A. Christopherson ◽  
Monica C. Remington ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Aluminum Hydroxide ◽  
Outer Surface ◽  
Protein A ◽  
Surface Protein ◽  
Major Complication ◽  
Sensu Stricto ◽  
Lyme Arthritis ◽  
Outer Surface Protein A ◽  
Outer Surface Protein

ABSTRACT Arthritis is a frequent and major complication of infection withBorrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30, 60, or 120 μg of recombinant OspA (rOspA) in aluminum hydroxide and challenged with B. burgdorferi sensu stricto isolate 297 or C-1-11. Swelling of the hind paws was detected in 100, 100, and 50% of hamsters vaccinated with 30, 60, or 120 μg of rOspA, respectively. In addition, arthritis developed in 57% of hamsters vaccinated with a canine rOspA vaccine after infection with B. burgdorferisensu stricto. When the canine rOspA vaccine was combined with aluminum hydroxide, all vaccinated hamsters developed arthritis after challenge with B. burgdorferi sensu stricto. Histopathologic examination confirmed the development of severe destructive arthritis in rOspA-vaccinated hamsters challenged with B. burgdorferisensu stricto. These findings suggest that rOspA vaccines should be modified to eliminate epitopes of OspA responsible for the induction of arthritis. Our results are important because an rOspA vaccine in aluminum hydroxide was approved by the Food and Drug Administration for use in humans.

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