Polymorphonuclear leucocyte killing activity of Candida albicans in newborns

1997 ◽  
Vol 56 (1-3) ◽  
pp. 115
Author(s):  
L Siegfried
Keyword(s):  
Candida Albicans ◽  
Polymorphonuclear Leucocyte ◽  
Killing Activity

Polymorphonuclear leucocyte killing activity of Candida albicans in newborns

1997 ◽  
Vol 56 ◽  
pp. 115
Author(s):  
L. Siegfried ◽  
L. Várady ◽  
M. Hulíková
Keyword(s):  
Candida Albicans ◽  
Polymorphonuclear Leucocyte ◽  
Killing Activity

Inflammatory mediators are involved in the Candida albicans killing activity of human epidermal cells

1990 ◽  
Vol 282 (5) ◽  
pp. 348-350 ◽  
Author(s):  
M. Csato ◽  
A. Sz. Kenderessy ◽  
R. Jud�k ◽  
A. Dobozy
Keyword(s):  
Candida Albicans ◽  
Inflammatory Mediators ◽  
Epidermal Cells ◽  
Killing Activity ◽  
Human Epidermal Cells

Regulation of Candida albicans killing activity of human epidermal cells

1987 ◽  
Vol 34 (2) ◽  
pp. 159
Author(s):  
A. Dobozy ◽  
J. Hunyadi ◽  
M. Csató
Keyword(s):  
Candida Albicans ◽  
Epidermal Cells ◽  
Killing Activity ◽  
Human Epidermal Cells

Killing Activity of Micafungin Against Candida albicans, C. dubliniensis and Candida africana in the Presence of Human Serum

2017 ◽  
Vol 182 (11-12) ◽  
pp. 979-987 ◽  
Author(s):  
Renátó Kovács ◽  
Qasem Saleh ◽  
Aliz Bozó ◽  
Zoltán Tóth ◽  
Rudolf Gesztelyi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Killing Activity ◽  
Candida Africana

Effect of Fluconazole on Human Polymorphonuclear Leucocyte Functions ex vivo against Candida albicans

Chemotherapy ◽  
1999 ◽  
Vol 45 (4) ◽  
pp. 277-283 ◽  
Author(s):  
Ümran Soyoǧul Gürer ◽  
Adile Çevikbaş ◽  
Candan Johansson ◽  
Koray Derici ◽  
Turay Yardımcı
Keyword(s):  
Candida Albicans ◽  
Ex Vivo ◽  
Polymorphonuclear Leucocyte

Effects of histatin 5 and derived peptides on Candida albicans

2001 ◽  
Vol 356 (2) ◽  
pp. 361-368 ◽  
Author(s):  
Anita L. A. RUISSEN ◽  
Jasper GROENINK ◽  
Eva J. HELMERHORST ◽  
Els WALGREEN-WETERINGS ◽  
Wim van't HOF ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Mitochondrial Membrane ◽  
Cytoplasmic Membrane ◽  
Human Saliva ◽  
Mitochondrial Activity ◽  
Complete Protection ◽  
Destructive Effect ◽  
Histatin 5 ◽  
Killing Activity ◽  
Anti Fungal

Three anti-microbial peptides were compared with respect to their killing activity against Candida albicans and their ability to disturb its cellular and internal membranes. Histatin 5 is an anti-fungal peptide occurring naturally in human saliva, while dhvar4 and dhvar5 are variants of its active domain, with increased anti-microbial activity. dhvar4has increased amphipathicity compared with histatin 5, whereas dhvar5has amphipathicity comparable with that of histatin 5. All three peptides caused depolarization of the cytoplasmic and/or mitochondrial membrane, indicating membranolytic activity. For the variant peptides both depolarization and killing occurred at a faster rate. With FITC-labelled peptides, no association with the cytoplasmic membrane was observed, contradicting the formation of permanent transmembrane multimeric peptide pores. Instead, the peptides were internalized and act on internal membranes, as demonstrated with mitochondrion- and vacuole-specific markers. In comparison with histatin 5, the variant peptides showed a more destructive effect on mitochondria. Entry of the peptides and subsequent killing were dependent on the metabolic state of the cells. Blocking of the mitochondrial activity led to complete protection against histatin 5 activity, whereas that of dhvar4 was hardly affected and that of dhvar5 was affected only intermediately.

scholarly journals Candida albicans Mutants Deficient in Respiration Are Resistant to the Small Cationic Salivary Antimicrobial Peptide Histatin 5

2000 ◽  
Vol 44 (2) ◽  
pp. 348-354 ◽  
Author(s):  
Csilla Gyurko ◽  
Urs Lendenmann ◽  
Robert F. Troxler ◽  
Frank G. Oppenheim
Keyword(s):  
Candida Albicans ◽  
Sodium Dodecyl ◽  
Atp Synthesis ◽  
Dependent Manner ◽  
Wild Type ◽  
Concentration Dependent Manner ◽  
Histatin 5 ◽  
Cellular Energy ◽  
Killing Activity ◽  
Petite Mutants

ABSTRACT Histatins are a group of small cationic peptides in human saliva which are well known for their antibacterial and antifungal activities. In a previous study we demonstrated that histatin 5 kills both blastoconidia and germ tubes of Candida albicans in a time- and concentration-dependent manner at 37°C, whereas no killing was detected at 4°C. This indicated that killing activity depends on cellular energy. To test histatin 5 killing activity at lower cellular ATP levels at 37°C, respiratory mutants, or so-called petite mutants, of C. albicans were prepared. These mutants are deficient in respiration due to mutations in mitochondrial DNA. Mutants were initially identified by their small colony size and were further characterized with respect to colony morphology, growth characteristics, respiratory activity, and cytochrome spectra. The killing activity of histatin 5 at the highest concentration was only 28 to 30% against respiratory mutants, whereas 98% of the wild-type cells were killed. Furthermore, histatin 5 killing activity was also tested on wild-type cells in the presence of the respiratory inhibitor sodium azide or, alternatively, the uncoupler carbonyl cyanidem-chlorophenylhydrazone. In both cases histatin 5 killing activity was significantly reduced. Additionally, supernatants and pellets of cells incubated with histatin 5 in the presence or absence of inhibitors of mitochondrial ATP synthesis were analyzed by sodium dodecyl sulfate gel electrophoresis. It was observed that wild-type cells accumulated large amounts of histatin 5, while wild-type cells treated with inhibitors or petite mutants did not accumulate significant amounts of the peptide. These data showed first that cellular accumulation of histatin 5 is necessary for killing activity and second that accumulation of histatin 5 depends on the availability of cellular energy. Therefore, mitochondrial ATP synthesis is required for effective killing activity of histatin 5.

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