A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion1Note: Nucleotide sequence data reported in this paper are available in the EMBL, GenBank™ and DDJB databases under the accession number AF068065.1,2A portion of the amino acid sequence of GP900 was presented at the 47th annual meeting of the Society of Protozoologists and 3rd Workshop on opportunistic Protozoan Pathogens in Cleveland, Ohio, June 1994.2

Three genomic clones (Rplec2, Rplec5 and Rplec6) and a cDNA clone (LECRPA4) that encoded lectin or lectin-related polypeptides were isolated from Robinia pseudoacacia L. A comparison of the nucleotide sequences of Rplec2 and a previously reported cDNA for the subunit indicated that Rplec2 encoded the 29 kDa subunit of the inner-bark lectin RPbAI. Rplec5 encoded a polypeptide whose deduced amino acid sequence was 96.1% identical to that of a subunit of seed lectin. The amino acid sequence deduced from the open reading frame of Rplec6 showed 61.1% identity to that encoded by Rplec5. LECRPA4 was isolated from an inner bark cDNA library and appeared to encode the 26 kDa subunit of inner-bark lectin RPbAII. The expression patterns of the various genes in tissues were examined by the reverse transcriptase-polymerase chain reaction (RT-PCR) with appropriate primers. Rplec2 transcripts were detected in the inner bark and roots. Rplec5 transcripts were detected in the inner bark, seeds and roots. No Rplec6 transcripts were detected in all tissues examined. LECRPA4 transcripts were found in leaves and in the inner bark. The level of expression of Rplec2 in the inner bark appeared to be similar in samples collected in different years and from different trees, whereas levels of expression of Rplec5 and LECRPA4 varied. These results suggest the differential regulation of expression of members of the lectin gene family in tissues of R. pseudoacacia. The nucleotide sequence data reported herein will appear in the DDBJ, EMBL and GenBank Nucleotide Sequence Databases under the accession numbers AB 012632 (Rplec2), AB012633 (Rplec5), AB012634 (Rplec6) and AB012635 (LECRPA4).

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Cloning and characterization of kidney-specific promoter of human PTH/PTHrP receptor gene: Absence of mutation in patients with pseudohypoparathyroidism type Ib1Presented in part at the Annual Meeting of the American Society of Bone and Mineral Research, September, 1996.12The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank nucleotide sequence databases with the following accession number AB006200 and AB006201.2

Molecular and Cellular Endocrinology ◽

10.1016/s0303-7207(98)00092-6 ◽

1998 ◽

Vol 141 (1-2) ◽

pp. 41-47 ◽

Cited By ~ 12

Author(s):

Seiji Fukumoto ◽

Miyuki Suzawa ◽

Tomoko Kikuchi ◽

Toshio Matsumoto ◽

Shigeaki Kato ◽

...

Keyword(s):

Nucleotide Sequence ◽

Annual Meeting ◽

Sequence Data ◽

Receptor Gene ◽

Accession Number ◽

Mineral Research ◽

Nucleotide Sequence Data ◽

American Society ◽

Pthrp Receptor

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Molecular Cloning, Nucleotide Sequence, and Expression in Escherichia coli of a Hemolytic Toxin (Aerolysin) Gene from Aeromonas trota

Applied and Environmental Microbiology ◽

10.1128/aem.64.7.2473-2478.1998 ◽

1998 ◽

Vol 64 (7) ◽

pp. 2473-2478 ◽

Cited By ~ 19

Author(s):

Ashraf A. Khan ◽

Eungbin Kim ◽

Carl E. Cerniglia

Keyword(s):

Escherichia Coli ◽

Amino Acid ◽

Nucleotide Sequence ◽

Amino Acid Sequence ◽

Sequence Data ◽

Kanamycin Resistance ◽

Aeromonas Salmonicida ◽

Amino Acid Sequences ◽

Nucleotide Sequence Data ◽

Hemolytic Toxin

ABSTRACT Aeromonas trota AK2, which was derived from ATCC 49659 and produces the extracellular pore-forming hemolytic toxin aerolysin, was mutagenized with the transposon mini-Tn5Km1 to generate a hemolysin-deficient mutant, designated strain AK253. Southern blotting data indicated that an 8.7-kb NotI fragment of the genomic DNA of strain AK253 contained the kanamycin resistance gene of mini-Tn5Km1. The 8.7-kb NotI DNA fragment was cloned into the vector pGEM5Zf(−) by selecting for kanamycin resistance, and the resultant clone, pAK71, showed aerolysin activity in Escherichia coli JM109. The nucleotide sequence of the aerA gene, located on the 1.8-kbApaI-EcoRI fragment, was determined to consist of 1,479 bp and to have an ATG initiation codon and a TAA termination codon. An in vitro coupled transcription-translation analysis of the 1.8-kb region suggested that the aerA gene codes for a 54-kDa protein, in agreement with nucleotide sequence data. The deduced amino acid sequence of the aerA gene product ofA. trota exhibited 99% homology with the amino acid sequence of the aerA product of Aeromonas sobria AB3 and 57% homology with the amino acid sequences of the products of the aerA genes of Aeromonas salmonicida 17-2 and A. sobria 33.

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