Genetic approaches to nuclear pore structure and function

How individual nucleoporins (Nups) perform their role in nuclear pore structure and function is largely unknown. In this study, we examined the structure of purified Nup170 to obtain clues about its function. We show that Nup170 adopts a crescent moon shape with two structurally distinct and separable domains, a β-propeller N terminus and an α-solenoid C terminus. To address the individual roles of each domain, we expressed these domains separately in yeast. Notably, overexpression of the Nup170 C domain was toxic in nup170Δ cells and caused accumulation of several Nups in cytoplasmic foci. Further experiments indicated that the C-terminal domain anchors Nup170 to nuclear pores, whereas the N-terminal domain functions to recruit or retain a subset of Nups, including Nup159, Nup188, and Pom34, at nuclear pores. We conclude that Nup170 performs its role as a structural adapter between cytoplasmically oriented Nups and the nuclear pore membrane.

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A role for the divergent actin gene, ACT2, in nuclear pore structure and function

The EMBO Journal ◽

10.1093/emboj/16.12.3572 ◽

1997 ◽

Vol 16 (12) ◽

pp. 3572-3586 ◽

Cited By ~ 20

Author(s):

C. Yan

Keyword(s):

Pore Structure ◽

Structure And Function ◽

Nuclear Pore ◽

Actin Gene ◽

And Function

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Faculty Opinions recommendation of Regulated and unregulated mitochondrial permeability transition pores: a new paradigm of pore structure and function?

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1004351.49904 ◽

2002 ◽

Author(s):

Eduardo Rial

Keyword(s):

Pore Structure ◽

Mitochondrial Permeability Transition ◽

Permeability Transition ◽

Structure And Function ◽

New Paradigm ◽

Mitochondrial Permeability ◽

Permeability Transition Pores ◽

And Function

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Research Progress on Preparation and Modification of Polyvinylidene Fluoride Membrane

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.549.25 ◽

2012 ◽

Vol 549 ◽

pp. 25-29

Author(s):

Ying Jie Zhang ◽

Ming Yuan Yuan ◽

Ru Lin

Keyword(s):

Pore Structure ◽

Membrane Structure ◽

Polyvinylidene Fluoride ◽

Structure And Function ◽

Research Progress ◽

Catalytic Membrane ◽

Preparation Methods ◽

Membrane Pore ◽

Pvdf Membrane ◽

And Function

Immersion precipitation is one of the commonly used methods in preparing polyvinylidene fluoride membrane (PVDF) membrane. Using this method, the membrane structure is easily controlled and the operation is very simple. This paper summarized the mechanism and preparation methods of PVDF as well as researching the effects of membrane-forming factors such as ingredient of solvents and non-solvents, temperature and additives etc. on the structure and function of PVDF membrane. Applying modification on PVDF membrane can improve its hydrophilicity and anti-fouling properties, of which blending modification is expected to be a hotspot of study in future. Adding catalytic agent into PVDF can generate catalytic membrane, which offers a researching direction for preparing multi-functional PVDF membrane. Finally, brief comments are made on the improvement of membrane pore structure and problem of singleness of blending materials.

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