Inhibition of kainic acid induced expression of interleukin-1β and interleukin-1 receptor antagonist mRNA in the rat brain by NMDA receptor antagonists

2000 ◽  
Vol 85 (1-2) ◽  
pp. 103-113 ◽  
Author(s):  
Charlotta Eriksson ◽  
Li-Ping Zou ◽  
Sven Ahlenius ◽  
Bengt Winblad ◽  
Marianne Schultzberg
Keyword(s):  
Nmda Receptor ◽  
Receptor Antagonist ◽  
Rat Brain ◽  
Kainic Acid ◽  
Interleukin 1 ◽  
Interleukin 1Β ◽  
Nmda Receptor Antagonists ◽  
Receptor Antagonists ◽  
Induced Expression ◽  
Interleukin 1 Receptor Antagonist

Use of Both Fluoro-Jade B and Hematoxylin and Eosin to Detect Cell Death in the Juvenile Rat Brain Exposed to NMDA-Receptor Antagonists or GABA-Receptor Agonists in Safety Assessment

2021 ◽  
pp. 019262332110077
Author(s):  
Catherine A. Picut ◽  
Odete R. Mendes ◽  
David S. Weil ◽  
Sarah Davis ◽  
Cynthia Swanson
Keyword(s):  
Cell Death ◽  
Nmda Receptor ◽  
Rat Brain ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Nmda Receptor Antagonists ◽  
Neonatal Rats ◽  
Receptor Antagonists ◽  
Fluoro Jade B ◽  
Hematoxylin And Eosin

Administration of pediatric anesthetics with N-methyl D-aspartate (NMDA)-receptor antagonist and/or γ-aminobutyric acid (GABA) agonist activities may result in neuronal degeneration and/or neuronal cell death in neonatal rats. Evaluating pediatric drug candidates for this potential neurotoxicity is often part of overall preclinical new drug development strategy. This specialized assessment may require dosing neonatal rats at postnatal day 7 at the peak of the brain growth spurt and evaluating brain tissue 24 to 48 hours following dosing. The need to identify methods to aid in the accurate and reproducible detection of lesions associated with this type of neurotoxic profile is paramount for meeting the changing needs of neuropathology assessment and addressing emerging challenges in the neuroscience field. We document the use of Fluoro-Jade B (FJB) staining, to be used in conjunction with standard hematoxylin and eosin staining, to detect acute neurodegeneration and neuronal cell death that can be caused by some NMDA-receptor antagonists and/or GABA agonists in the neonatal rat brain. The FJB staining is simple, specific, and sensitive and can be performed on brain specimens from the same cohort of animals utilized for standard neurotoxicity assessment, thus satisfying animal welfare recommendations with no effect on achievement of scientific and regulatory goals.

Human interleukin-1β and interleukin-1 receptor antagonist secretion and velocity of tooth movement

2001 ◽  
Vol 46 (2) ◽  
pp. 185-189 ◽  
Author(s):  
L.R. Iwasaki ◽  
J.E. Haack ◽  
J.C. Nickel ◽  
R.A. Reinhardt ◽  
T.M. Petro
Keyword(s):  
Receptor Antagonist ◽  
Tooth Movement ◽  
Interleukin 1 ◽  
Interleukin 1Β ◽  
Interleukin 1 Receptor Antagonist
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