Development of a new method for the quantitative analysis of the extracellular polysaccharide of Neisseria meningitidis serogroup A by use of high-performance anion-exchange chromatography with pulsed-amperometric detection

Vaccine ◽  
2001 ◽  
Vol 19 (15-16) ◽  
pp. 1989-1997 ◽  
Author(s):  
Stefano Ricci ◽  
Angela Bardotti ◽  
Sandro D'Ascenzi ◽  
Neil Ravenscroft
Keyword(s):  
Quantitative Analysis ◽  
Neisseria Meningitidis ◽  
Anion Exchange ◽  
High Performance ◽  
Amperometric Detection ◽  
Extracellular Polysaccharide ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
New Method ◽  
Pulsed Amperometric Detection

scholarly journals Quantitative Analysis of Fructo-oligosaccharides in Gynura divaricata subsp. formosana by High Performance Anion Exchange Chromatography-Pulsed Amperometric Detection

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700
Author(s):  
Shen-Chieh Chou ◽  
Shoei-Sheng Lee
Keyword(s):  
Quantitative Analysis ◽  
Anion Exchange ◽  
High Performance ◽  
Amperometric Detection ◽  
Dry Weight ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Fresh Weight ◽  
Pulsed Amperometric Detection ◽  
Limit Of Quantitation

High performance anion exchange chromatography-pulsed amperometric detection was employed in this study to conduct quantitative analysis of the inulin-related fructo-oligosaccharides present in Gynura divaricata subsp. formosana. Result obtained for the 1-kestose (GF2), nystose (GF3) and 1F-β-fructofuranosyl-nystose (GF4) contents were 146.60 ± 0.04, 24.70 ± 0.75 and 16.60 ± 0.91 μg/g, fresh weight, and 68.90 ± 0.02, 7.60 ± 1.34 and 149.30 ± 0.06 μg/g (mean ± RSD), dry weight, respectively. Using this method, the limit of quantitation was 20 μg/mL and the linear detectability between 0-250 μg/mL. The developed method provides a practical analysis for these low caloric value, prebiotic and non-digestible carbohydrates in the genus Gynura.

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